Device and method used for measuring selenium in water bodies via molecular imprinting selective laser induced fluorescence

Abstract

The invention provides a device and a method used for measuring selenium in water bodies via molecular imprinting selective laser induced fluorescence. According to the device, a fluorescent pool is designed to be flat; the inner bottom of the fluorescent pool is coated with a molecularly imprinted polymer with absorption capacity on a complex formed from hydroxyethyl ethylenediamine triacetic acid and selenium IV in water bodies. According to the method, the complex is formed from selenium IV in water bodies and hydroxyethyl ethylenediamine triacetic acid, the molecularly imprinted polymer prepared by taking the selenium IV-hydroxyethyl ethylenediamine triacetic acid complex as a template molecule is capable of generating fluorescence at certain wave length under laser light source excitation, fluorescence signal strength and the concentration of selenium in water bodies are linearly associated, high efficiency selective enrichment is realized via molecular imprinting, and the method used for measuring selenium in water bodies is established via laser induced fluorescence method. When the device and the method are used for measuring selenium in water bodies, one-site detection can be realized, detection is rapid and simple, and sensitivity is high.

Description

technical field

[0001] The invention relates to the technical field of environmental chemical monitoring, in particular to a method for measuring selenium in water bodies. Background technique

[0002] Selenium is not only a toxic element for animals and human body, but also an essential nutrient element. It has been found that endemic cardiomyopathy often occurs in areas with low selenium, and high selenium causes cancer and other diseases, but an appropriate amount of selenium can enhance immunity. Selenium is also a natural antidote for some animals and marine organisms to heavy metals such as Hg, Cu, and Cd. Its toxicity and physiological effects are closely related to its valence and form. Therefore, the research on the analysis method of selenium valence state in seawater has attracted more and more attention of environmental analytical chemists. There are many determination methods for selenium content, such as 3,3-diaminoaniline spectrophotometry, 2,3-diaminonapht...

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