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Streptomyces praeterii and its application in the production of platymycin and platylin

A technology of Streptomyces platteri and platycillin, which is applied to Streptomyces platteri and its application fields, can solve the problem of low yield of platomycin and platydin, hindering the application research, development and utilization of platymycin and platin, etc. question

Active Publication Date: 2018-12-21
CHANGSHA CHARISM BIOSCIENCES CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, the output of platymycin and platylin in above-mentioned two strains is all very low, has hindered the applied research and development utilization of platymycin and platylin greatly

Method used

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  • Streptomyces praeterii and its application in the production of platymycin and platylin
  • Streptomyces praeterii and its application in the production of platymycin and platylin
  • Streptomyces praeterii and its application in the production of platymycin and platylin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 Obtaining of Streptomyces platetts CB00739, CB00765, CB00775, CB00789, CB02289 and CB02304 bacterial strains

[0069] The isolated samples were collected from soil samples in Sichuan, Yunnan and Heilongjiang in my country. After the collected soil samples are naturally air-dried for 5-10 days, they are ground and mixed with sterile water to form a soil suspension. Then the soil suspension was heated at 45°C-50°C for 10-20 minutes, diluted with sterile water and evenly spread on Gaoshi No. 1 medium (20 grams of soluble starch, 0.5 grams of dipotassium hydrogen phosphate, sulfuric acid heptahydrate 0.5 grams of magnesium, 0.5 grams of sodium chloride, 10 milligrams of ferrous sulfate, 20 grams of agar powder, pH 7.2), inverted 28 ℃ constant temperature and humidity culture, according to the colony shape selection may belong to actinomycetes, and can vigorously grow strains. After the obtained pure strain produced spores, the spores were collected in 20% glycero...

Embodiment 2

[0070] Embodiment 2 Fermentation of Streptomyces prater CB00739, CB00765, CB00775, CB00789, CB02289 and CB02304 strains

[0071] About 10 microliters of spores of Streptomyces praeterii strains CB00739, CB00765, CB00775, CB00789, CB02289 and CB02304 were inoculated into 50 mL of seed medium, respectively. The strains were cultured on a constant temperature shaker at 25-32°C, 200-240rpm for 36-60 hours, and then transferred to 50mL production medium with an inoculum size of 5% to 15%. Cultivate for 5-8 days. The seed medium formula (mass percentage): 1.0-2.0% of yeast powder, 0.5-1.2% of malt extract (purchased from Shanghai Sangong), 2-3% of glucose, 0.025-0.040% of magnesium sulfate, hexahydrate Ferric chloride 0.025-0.040%, the rest is water; pH6.5-7.5. The production medium formula: dextrin 2-6%, lactose 2-6%, yeast powder 0.3-0.8%, calcium carbonate 0.08-0.13%, 3-(N-morpholino)propanesulfonic acid 0.3-0.8% (mass percentage content), the rest is water; pH 6.5~7.5.

Embodiment 3

[0072] Embodiment 3 The chemical analysis of the fermentation product of Streptomyces prater CB00739, CB00765, CB00775, CB00789, CB02289 and CB02304 strains

[0073] After the fermentation broths of Streptomyces platetts strains CB00739, CB00765, CB00775, CB00789, CB02289 and CB02304 were centrifuged, the supernatant was added to 3 g of macroporous adsorption resin, and mixed at room temperature for 1-3 hours. The macroporous adsorption resin was washed with 3 X 30mL deionized water and dried in the shade. 1.5 g of the dried macroporous resin was impregnated with methanol, ethanol or acetone (3X 10mL), concentrated and analyzed by high performance liquid chromatography and liquid chromatography-mass spectrometry. By comparative analysis of flat mycin and flat standard sample ( Figure 1-2 ), and the retention time ( Figure 3-8 ), molecular ion peaks provided by liquid chromatography-mass spectrometry ( Figure 9-14 ), it was found that the fermentation products of CB00739,...

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Abstract

The invention belongs to the field of bacterial species, relates to Streptomyces praeteri and its application, and specifically provides Streptomyces praeteri and its application in the production of platymycin and platylin. Streptomyces teretes CB00739, S. platetts CB00765, S. platetts CB00775, S. platetts CB00739, S. platetts CB02289 and S. platetts CB02304.

Description

technical field [0001] The invention belongs to the field of new strains, and in particular relates to Streptomyces praeteri and its application. Background technique [0002] Drug-resistant pathogenic bacteria infection and diabetes are major diseases that seriously endanger human health. Due to the abuse of antibiotics, some drug-resistant strains have developed resistance to imipenem and vancomycin, which are known as the "last line of defense of antibiotics". Worldwide, only three types of new antibiotics have been marketed in recent decades, namely, linezolid of the oxazolidinone class, daptomycin of the lipopeptide class, and mulling antibiotics of the diterpene class. The development of antibacterial drugs with a new mechanism of action has become an urgent need of the whole society. Diabetes is known as the "silent killer" of human beings. In addition to high blood sugar, there are many complications such as retinopathy, kidney disease and neurological disorders. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/18C12P15/00C12R1/585
CPCC12P15/00C12P17/181C12N1/205C12R2001/585
Inventor 段燕文沈奔黄勇朱湘成
Owner CHANGSHA CHARISM BIOSCIENCES CO LTD