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Corium tissue bionic sponge preparation method

A tissue and sponge technology, applied in the field of medical bionic materials, can solve the problems of promoting functional dermal tissue regeneration, secondary surgery, virus transmission, etc., to promote adhesion and proliferation activity, and reduce biodegradation speed , Improve the effect of biocompatibility

Active Publication Date: 2015-11-11
WUXI NO 3 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, due to the difficulty in controlling the processing technology, the quality of acellular dermal matrix-like dermal biomimetic materials is unstable, and there are often quality differences between production batches. Serious consequences such as surgery
Secondly, there is a possibility of bio-contamination in the biomimetic material of acellular dermal matrix dermis, because the raw human cadaver skin may carry viruses, and these viruses are not effectively removed during material processing, resulting in the spread of viruses
Thirdly, the degradation rate of in vitro synthetic dermis bionic materials on the wound surface does not match the rate of tissue formation, and the effect of promoting wound repair and inhibiting scar formation is often weakened due to excessive degradation.
In addition, the effect of existing dermal bionic materials on inhibiting scar formation is far from achieving the goal of promoting the regeneration of functional dermis-like tissue

Method used

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  • Corium tissue bionic sponge preparation method
  • Corium tissue bionic sponge preparation method
  • Corium tissue bionic sponge preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Put 10g of silkworm cocoons in 500ml of 0.02MNa 2 CO 3 Boiled in the aqueous solution for 30min, then rinsed with deionized water for 3 times to obtain silk fibroin fiber; then placed the silk fibroin fiber in 10ml of 9.3M lithium bromide solution, dissolved at 37°C, and obtained silk protein lithium bromide solution; Finally, the obtained solution was dialyzed in a dialysis box with a molecular weight cut-off of 3500 Da for 3 days to obtain an aqueous solution of silk protein, and the mass percentage concentration of the solution was adjusted to 5%;

[0027] (2) Mix the silk protein solution prepared in step (1) with the collagen solution with a concentration of 1.5% by mass at 37° C. in a volume ratio of 7:10, on a vortex shaker with a speed of 50 rpm / min Shake for 5 minutes until the mixture is uniform, then centrifuge for 3 minutes at a centrifugal force of 1500 g to remove air bubbles in the solution;

[0028] (3) transfer the mixed solution after centrifugat...

Embodiment 2

[0033] (1) Place 10g of silkworm cocoons in 500ml of 1MNa 2 CO 3 Boiled in the aqueous solution for 30min, then rinsed with deionized water for 3 times to obtain silk fibroin fibers; then placed the silk fibroin fibers in 10ml of 10M lithium bromide solution, and dissolved at 37°C to obtain silk protein lithium bromide solution; After the obtained solution was dialyzed in a dialysis box with a molecular weight cut-off of 3500 Da for 3 days, an aqueous solution of silk protein was obtained, and the mass percentage concentration of the solution was adjusted to 5%;

[0034] (2) Mix the silk protein solution prepared in step (1) with the collagen solution with a mass percentage concentration of 1.5% at a volume ratio of 57:10 at 37° C., on a vortex shaker with a speed of 50 rpm / min Shake for 5 minutes until the mixture is uniform, and then centrifuge for 3 minutes at a centrifugal force of 1500 g to remove air bubbles in the solution;

[0035] (3) transfer the mixed solution aft...

Embodiment 3

[0039] (1) Put 10g of silkworm cocoons in 500ml of 0.05MNa 2 CO 3 Boiled in the aqueous solution for 30min, then rinsed with deionized water for 3 times to obtain silk fibroin fibers; then placed the silk fibroin fibers in 10ml of 15M lithium bromide solution, and dissolved at 37 °C to obtain silk protein lithium bromide solution; After the obtained solution was dialyzed in a dialysis box with a molecular weight cut-off of 3500 Da for 3 days, an aqueous solution of silk protein was obtained, and the mass percentage concentration of the solution was adjusted to 5%;

[0040] (2) Mix the silk protein solution prepared in step (1) with the collagen solution with a mass percentage concentration of 1.5% at a volume ratio of 0.4:10 at 37° C., on a vortex shaker with a speed of 50 rpm / min Shake for 5 minutes until the mixture is uniform, and then centrifuge for 3 minutes at a centrifugal force of 1500 g to remove air bubbles in the solution;

[0041] (3) transfer the mixed solution ...

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Abstract

The invention discloses a corium tissue bionic sponge preparation method. The mass percent of silk protein in a corium tissue bionic sponge is 10%-95%, the mass percent of collagen is 5%-90%, a silk protein solution and a collagen solution are mixed and steamed through steam to form a film, then the film is soaked in a mixed aqueous solution of 1-(3-dimethylamino propyl)-3-ethyl-carbodiimide hydrochloride and N-hydroxy succinimide, and the corium tissue bionic sponge is prepared through the drying process and other processes. The corium tissue bionic sponge has the corium tissue bionic performance in the aspect of microstructure, mechanical property and molecular structure, can easily improve the performance of inhibiting scar formation and promoting functional corium tissue regeneration, and provides a new method for solving the problem of pathologic scars.

Description

technical field [0001] The invention relates to the technical field of medical biomimetic materials, in particular to a method for preparing a dermal tissue biomimetic sponge by using silk protein and collagen as raw materials. Background technique [0002] Skin defects caused by trauma such as burns, wounds, and surgical incisions are common and frequently-occurring diseases in clinical practice. In the process of skin defect repair, excessive wound healing will lead to the occurrence and development of pathological scars. Pathological scars, including hypertrophic scars and keloids, can cause a series of serious complications such as itching, disfigurement, disability, and sometimes even induce malignant tumors, which seriously affect the quality of life of patients. Therefore, the prevention and treatment of pathological scars has always been an urgent problem to be solved in the field of surgery such as burns and plastic surgery. [0003] In recent years, with the deepe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/56
Inventor 赵朋吕国忠
Owner WUXI NO 3 PEOPLES HOSPITAL
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