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Method for preparing egg yolk lecithin used for injection through aluminum oxide static adsorption impurity removal and low-temperature sediment oil removal

A technology of egg yolk lecithin and static adsorption, applied in the direction of edible phospholipid composition, application, protein food ingredients, etc., can solve the problems of large amount of deoiling solvent, difficult removal of impurities, difficult recovery and reuse of deoiling solvent, etc., to achieve solvent The effect of less dosage, easy operation and low cost

Active Publication Date: 2015-11-11
SHENYANG TIANFENG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the above deficiencies in the prior art, the present invention provides a method for preparing egg yolk lecithin for injection by static adsorption of alumina to remove impurities and low-temperature precipitation deoiling, which solves the difficulty of removing impurities such as LPE and PI in the lecithin refining process , The amount of deoiling solvent is large, and the deoiling solvent is not easy to recycle and reuse.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 600g of egg yolk powder and place it in a 5000ml Erlenmeyer flask, add 2400ml of absolute ethanol, stir and leach for 2 hours when the liquid temperature is 40°C, filter cloth; add 1800ml of absolute ethanol to the filter cake, Stir and extract at 40°C for 2 hours, filter the cloth, combine the two extracts, concentrate under reduced pressure at 50°C to obtain a concentrated solution, let stand until complete separation, and remove the lower oil phase.

[0022] Add 48g of 200-300 mesh neutral alumina to the concentrated solution, raise the temperature of the liquid to 25°C, stir and absorb for 1 hour, pass through a 0.45 μm filter membrane, concentrate the filtrate at 50°C under reduced pressure, and dry it in vacuum, which is the ethanol leached dry product, mass It is 230g.

[0023] Add 2300ml of mixed solvent of n-hexane: acetone = 1:5 to the ethanol extraction dry product, heat to 35°C under stirring condition to dissolve, continue stirring at this temperature ...

Embodiment 2

[0026] Take 600g of egg yolk powder and place it in a 5000ml Erlenmeyer flask, add 3000ml of 95% ethanol, stir and extract for 50min when the liquid temperature is 25°C, filter cloth; Add 1800ml95% ethanol to the filter cake, stir and extract 50min when the liquid temperature is 25°C, filter cloth; add 1800ml95% ethanol to the filter cake, Stir and extract at 25°C for 50 minutes, filter cloth. The four extracts were combined and concentrated under reduced pressure at 50°C to obtain a concentrated solution, which was allowed to stand until the layers were completely separated, and the lower oil phase was removed.

[0027] Add 60g of 200-300 mesh neutral alumina to the concentrated solution, raise the temperature of the liquid to 40°C, stir and absorb for 3 hours, pass through a 0.45 μm filter membrane, concentrate the filtrate under reduced pressure at 50°C, and dry it in vacuum, which is the ethanol leached dry matter, mass It is 249g.

[0028] Add 2241ml of n-hexane: aceton...

Embodiment 3

[0031]Take 600g egg yolk powder and place it in a 5000ml Erlenmeyer flask, add 2400ml99% ethanol, stir and extract for 90min when the liquid temperature is 30°C, filter cloth; Stir and extract for 90 minutes, filter cloth; add 1800ml of 99% ethanol to the filter cake, stir and extract for 90 minutes when the liquid temperature is 30°C, filter cloth. The three extracts were combined and concentrated under reduced pressure at 50°C to obtain a concentrated solution, which was allowed to stand until the layers were completely separated, and the lower oil phase was removed.

[0032] Add 54g of 60-180 mesh neutral alumina to the concentrated solution, raise the temperature of the drug solution to 33°C, stir and absorb for 1.5h, pass through a 0.22μm filter membrane, concentrate the filtrate at 50°C under reduced pressure, and dry it in vacuum, which is the ethanol extraction dry matter. The mass is 234g.

[0033] Add 2574ml of mixed solvent of n-hexane: acetone = 1:7 to the ethanol...

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Abstract

The invention discloses a method for preparing egg yolk lecithin used for injection through aluminum oxide static adsorption impurity removal and low-temperature sediment oil removal, and belongs to the technical field of egg yolk lecithin purification. The method includes the steps of conducting extraction on egg yolk powder or egg yolk particles serving as raw materials through absolute ethyl alcohol, conducting aluminum oxide static adsorption, conducting dissolving through a hexane-acetone mixed solution, conducting low-temperature sedimentation to separate out lecithin, and conducting sedimentation and drying to obtain the product. The thin layer chromatography detection on the product shows that residues of triglyceride, cholesterol and palmitic acid meet the limit requirements of Chinese Pharmacopoeia of the 2015 version. A brand-new process method is provided for impurity removal and oil removal of egg yolk lecithin and is high in yield and good in impurity removal and oil removal effect. Labor hours used for aluminum oxide static adsorption are short, operation is easy and convenient, and LPE, PI and other impurities can be smoothly removed. Devices are simple, the fixed-asset investment is small, solvent is easy to recycle, and emission of three wastes is greatly reduced.

Description

technical field [0001] The invention relates to the technical fields of separation and purification and lecithin, in particular to a method for preparing egg yolk lecithin for injection by static adsorption of alumina and deoiling by low-temperature precipitation. Background technique [0002] Phospholipids are ubiquitous in various tissues and organs of the human body, especially in the brain, nervous system, heart, lung, kidney, spleen, blood, and emulsion, and are components of biofilms. Egg yolk lecithin for injection has high nutritional value and physiological activity, and has significant curative effects in protecting cell membranes, anti-aging, lowering blood fat, and preventing and treating fatty liver. In addition to biological functions and pharmaceutical applications, phospholipids are widely used in food industry, light industry, chemical industry and other fields, because phospholipids also have a variety of functional characteristics, such as emulsification, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 王志强王继文李津鞠加林
Owner SHENYANG TIANFENG BIOLOGICAL PHARMA
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