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A method for ultrafiltration treatment of flavomycin fermentation broth

A flavomycin and fermentation broth technology, which is applied in the field of ultrafiltration treatment of flavomycin fermentation broth, can solve the problems such as low actual effect of ultrafiltration method, and achieve the effects of shortened operation process, simple process and low energy consumption.

Inactive Publication Date: 2017-10-27
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a modern industrial application of the principle of dialysis, U.S. Patent No. 5,986,089 also explains in detail the process of ultrafiltration for the separation and purification of flavomycin in the examples. However, based on the data in the examples, the actual effect of ultrafiltration is inferior to that of macropores Adsorption method

Method used

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  • A method for ultrafiltration treatment of flavomycin fermentation broth
  • A method for ultrafiltration treatment of flavomycin fermentation broth

Examples

Experimental program
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Effect test

Embodiment 1

[0059] A method for ultrafiltration treatment of flavomycin fermentation broth, comprising the following steps:

[0060] 1) Put the flavomycin fermentation broth (solid content: 30g / L) in the refrigerator (4°C) overnight, centrifuge at 8000r / min for 10min, remove the sediment, and use the supernatant for later use;

[0061] 2) Take 400mL of the supernatant and use deionized water for continuous ultrafiltration for 1h at a pressure of 25psi;

[0062] 3) Continue ultrafiltration of the ultrafiltrate with a 1% urea aqueous solution having a pH value of 9.0 for 1.0 h, and collect 1200 mL of the ultrafiltrate;

[0063] Polysulfone ultrafiltration membrane is used for ultrafiltration, and the molecular cutoff of ultrafiltration membrane is 10000;

[0064] Check the yield and purity. The purity of the flavomycin A fraction was 51.5%, and the yield was 57.4%.

[0065] 4) Take 400 mL of the dialysate enriched in flavomycin after ultrafiltration, adjust the pH=3.0, and pump it into a...

Embodiment 2

[0068] A method for ultrafiltration treatment of flavomycin fermentation broth, comprising the following steps:

[0069] 1) Put the flavomycin fermentation broth (solid content: 30g / L) in the refrigerator (4°C) overnight, centrifuge at 8000r / min for 10min, remove the sediment, and use the supernatant for later use;

[0070] 2) Take 400mL supernatant and use deionized water for continuous ultrafiltration for 1h, the pressure is 25psi, and collect the filtrate;

[0071] 3) Continue ultrafiltration of the filtrate with a 2% aqueous methanol solution with a pH value of 7.5 for 1.0 h, and collect 1200 mL of ultrafiltrate;

[0072] Polyacrylonitrile ultrafiltration membrane is used for ultrafiltration, and the molecular cutoff of ultrafiltration membrane is 30000;

[0073] Check the yield and purity. The purity of the flavomycin A fraction was 48.3%, and the yield was 59.7%.

[0074] 4) Take 400 mL of flavomycin-enriched dialysate after ultrafiltration, adjust pH=3.5, and pump it...

Embodiment 3

[0077] A method for ultrafiltration treatment of flavomycin fermentation broth, comprising the following steps:

[0078] 1) Put the flavomycin fermentation broth (solid content: 30g / L) in the refrigerator (4°C) overnight, centrifuge at 8000r / min for 10min, remove the sediment, and use the supernatant for later use;

[0079] 2) Take 400mL supernatant and use deionized water for continuous ultrafiltration for 1h, the pressure is 25psi, and collect the filtrate;

[0080] 3) Continue ultrafiltration of the filtrate with a volume fraction of 1.5% ethylene glycol aqueous solution with a pH value of 7.0 for 1.0 h, and collect 1200 mL of ultrafiltrate;

[0081] The polyamide ultrafiltration membrane is selected for ultrafiltration, and the molecular cutoff of the ultrafiltration membrane is 50,000;

[0082] Check the yield and purity. The purity of the flavomycin A fraction was 46.3%, and the yield was 60.4%.

[0083] 4) Take 400 mL of the dialysate enriched in flavomycin after ult...

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Abstract

The invention discloses a method for ultrafiltration treatment of flavin fermentation broth, belonging to the technical field of biochemical industry, comprising the following steps: 1) continuous ultrafiltration of flavin fermentation broth with deionized water for 1 hour, and discarding dialysate; 2 ) Continue ultrafiltration for 1 h with the aqueous solution containing micelle regulator for the flavomycin fermentation broth retained by ultrafiltration in step 1), and collect the dialysate enriched with flavomycin. The ultrafiltration method adopted in the present invention first removes small molecular impurities in the fermentation broth by deionized water, then continues ultrafiltration to remove macromolecular impurities by adding an aqueous solution of a micellar regulator, and further chromatographs the ultrafiltrate rich in flavomycin. Refined by the method to obtain the flavomycin A component. Compared with the existing process, the process is simple, the operation process is greatly shortened, the energy consumption is low, and it is green and environmentally friendly, and is suitable for industrial scale production.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for treating flavomycin fermentation liquid by ultrafiltration. Background technique [0002] Flavomycin, also known as moenomycin, is a phosphoglycolipid antibiotic, a fermentation product of Streptomyces bemberii, and a common name for some structural analogues. Flavomycin has a strong effect on Gram-positive bacteria, and its pharmacological effect lies in interfering with the biosynthesis of peptidoglycan, causing bacterial cell rupture and causing bacterial autolysis. For a long time, flavomycin has been used in various countries in the world mainly as an antibiotic livestock and poultry growth promoter. Chinese patent CN1194984A discloses a new application of flavomycin, flavomycin and its bismuth salt composition can control the infection of Helicobacter pylori and treat various stomach diseases such as gastric ulcer. In order to reduce the side effec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/12C07H1/06
Inventor 陈斌李蓉俱名扬郭燕彬杨凯迪马晓迅
Owner NORTHWEST UNIV
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