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Rhodococcus strain C3, microbial agent containing rhodococcus strain C3, and applications of rhodococcus strain C3 and microbial agent

A technology of microbial agents and strains, applied in the field of bioaugmentation of environmental pollutants, can solve the problems of unstable treatment effect, long sludge domestication time, poor impact resistance, etc., and achieve good solid-liquid separation effect and high-purity strains Efficient and simple method

Inactive Publication Date: 2015-11-11
CNOOC TIANJIN CHEM RES & DESIGN INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the existence of high-concentration phenolic substances, traditional activated sludge method and other biological treatment technologies have problems such as long sludge domestication time, unstable treatment effect, and poor impact resistance.

Method used

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  • Rhodococcus strain C3, microbial agent containing rhodococcus strain C3, and applications of rhodococcus strain C3 and microbial agent
  • Rhodococcus strain C3, microbial agent containing rhodococcus strain C3, and applications of rhodococcus strain C3 and microbial agent
  • Rhodococcus strain C3, microbial agent containing rhodococcus strain C3, and applications of rhodococcus strain C3 and microbial agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the acquisition and identification of rhodococcus strain C3 bacterial strain

[0030] 1. Acquisition of strains

[0031] The strain originated from the activated sludge of the coking plant wastewater biological treatment system. Draw 2 ml of sludge samples into 200 ml of fresh enrichment medium with a phenol concentration of 200 mg / L according to 1% inoculum amount, and culture on a shaking table (30° C., 200 r / min). After the enrichment bottle is turbid, take 2ml of the culture solution and transfer it to a liquid medium with a phenol concentration of 400mg / L for cultivation. By analogy, gradually increase the phenol concentration to 1000mg / L. The cells enriched in the enrichment medium with a phenol concentration of 1000 mg / L were transferred to an inorganic salt medium with a phenol concentration of 1000 mg / L for acclimation. The strains using phenol as the sole carbon and energy source were isolated and screened on the separation medium plate by dil...

Embodiment 2

[0038] Embodiment 2: Rhodococcus strain RhodococcusC3 is to the degradation of pyridine and o-cresol in inorganic salt medium

[0039] Pick a single colony of Rhodococcus strain C3 and put it in 3ml LB liquid medium, culture it with shaking at 30°C and 200r / min for 24 hours to obtain a fresh bacterial solution. Pyridine and o-cresol were added to the inorganic salt medium respectively, and the final concentrations of pyridine and o-cresol were respectively 100 mg / L. The degradation results are shown in Table 1.

[0040] substrate pyridine o-cresol Degradation rate% 100 100

Embodiment 3

[0041] Example 3: Degradation of phenol, pyridine and o-cresol in coking wastewater of Rhodococcus strain C3

[0042] Measure 100ml of coking wastewater, the concentration of phenol is 350±5mg / L; add pyridine and o-cresol to make the concentration respectively 100±5mg / L, insert 2ml of fresh bacterial liquid, mix well, place at 30℃, 200r / Min shaker culture, 5 days later (120h) samples were taken to determine the degradation rate of the compound. The results are shown in Table 2.

[0043] substrate phenol pyridine o-cresol Degradation rate% 100 >90 >90

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Abstract

The invention discloses a rhodococcus strain C3. A preparation method of the rhodococcus strain C3 comprises the following steps of sucking an activated sludge sample from a wastewater biological treatment system of a coking plant to a fresh enrichment culture medium of phenol for shaking culture, wherein the inoculum concentration is 1%; after an enrichment bottle becomes muddy, taking a certain amount of the culture solution, inoculating a phenol liquid culture medium with increased concentration with the culture solution for culturing; in the same way, gradually increasing the concentration of phenol to 1000mg / L; inoculating an inorganic salt culture medium with the concentration of phenol being 1000mg / L with the strain enriched by the enrichment culture medium for domestication; and separating and screening a strain taking phenol as the unique carbon source and energy source from an isolation medium plate by adopting a dilution spread plate method and a streak plate A single colony of the strain is separated from an LB solid medium in a streaking manner, wherein the single colony is yellowish and semitransparent, has a neat edge and wet texture, and is easy to prick up; phenol, pyridine and o-cresol are taken as carbon sources, and the strain can degrade 1000mg / L of phenol in the inorganic salt liquid culture medium. The invention further provides a microbial agent taking rhodococcus strain C3 as the active component, and a preparation method of the microbial agent.

Description

technical field [0001] The invention belongs to the technical field of bioenhancement of environmental pollutants, specifically relates to a rhodococcus and its application in wastewater treatment, and particularly relates to the biological treatment of phenolic organic pollutants. Background technique [0002] Phenol is a highly toxic substance, which has a strong corrosive effect on the skin and mucous membranes, and can inhibit the central nervous system or damage the liver and kidney functions. High concentrations of phenol can coagulate proteins and cause tissue damage and necrosis. In water, 5-25mg / L of phenol can pose a threat to the survival of fish. Due to the potential toxicity of phenol, which is carcinogenic, teratogenic, and mutagenic, countries have successively proposed to list phenol as a priority pollutant in water. The phenolic substances mainly come from petroleum refining, coal coking plants, and gas plants. If such wastewater is discharged in large quan...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/08C12N11/04C02F3/34C12R1/01C02F101/34
Inventor 郑贝贝霍莹杨勇付连超张莹
Owner CNOOC TIANJIN CHEM RES & DESIGN INST
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