Rhodococcus strain C3, microbial agent containing rhodococcus strain C3, and applications of rhodococcus strain C3 and microbial agent
A technology of microbial agents and strains, applied in the field of bioaugmentation of environmental pollutants, can solve the problems of unstable treatment effect, long sludge domestication time, poor impact resistance, etc., and achieve good solid-liquid separation effect and high-purity strains Efficient and simple method
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[0029] Example 1: Acquisition and identification of Rhodococcus C3 strain
[0030] 1. Acquisition of strains
[0031] The strain originated from the activated sludge of the wastewater biological treatment system of the coking plant. Take 2ml sludge sample according to 1% inoculum to 200ml fresh enriched medium with phenol concentration of 200mg / L, shake culture (30℃, 200r / min) on a shaker. After the enrichment flask is turbid, take 2ml of culture solution and transfer it to a liquid culture medium with a phenol concentration of 400mg / L. By analogy, gradually increase the phenol concentration to 1000 mg / L. The bacteria after being enriched in an enrichment medium with a phenol concentration of 1000 mg / L are transferred to an inorganic salt medium with a phenol concentration of 1000 mg / L for acclimatization. Using the method of dilution coating and streaking, the strains with phenol as the sole carbon source and energy source are separated and screened on the separation medium pla...
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[0038] Example 2: Rhodococcus C3 degradation of pyridine and o-cresol in inorganic salt medium
[0039] Pick a single colony of Rhodococcus strain C3 in 3ml LB liquid medium and culture it with shaking at 30°C and 200r / min for 24 hours to obtain a fresh bacterial solution. Pyridine and o-cresol were added to the inorganic salt medium, and the final concentrations of pyridine and o-cresol were 100 mg / L. The degradation results are shown in Table 1.
[0040] Substrate Pyridine O-cresol Degradation rate%100100
Example Embodiment
[0041] Example 3: Degradation of phenol, pyridine and o-cresol in coking wastewater by Rhodococcus strain C3
[0042] Measure 100ml of coking wastewater, the concentration of phenol is 350±5mg / L; add pyridine and o-cresol to make the concentration of 100±5mg / L respectively, insert 2ml of fresh bacterial liquid, mix, and place at 30℃, 200r / Min shaker culture, 5 days later (120h) sampling to determine the degradation rate of the compound. The results are shown in Table 2.
[0043] Substrate phenol Pyridine O-cresol Degradation rate%100> 90 > 90
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