Preparation method of fish collagen peptide compound

A fish collagen and collagen technology, which is applied in the field of preparation of fish collagen short peptide complexes, can solve problems such as lack of collagen peptide complex extraction process, and achieve the effects of easy operation, high extraction rate and simple process

Active Publication Date: 2015-11-11
海南盛美诺生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After a detailed analysis of a large number of documents on the extraction process of collagen peptides, we found that the fish skin collagen peptides extracted according to the general method have a certain fishy and bitter taste, and the research on the composite extraction process of collagen peptides is still relatively lacking. , especially the use of plant enzymes in fruit and vegetable juices to enzymatically hydrolyze collagen peptides, and then the composite treatment method of microbial fermentation has not yet been reported

Method used

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  • Preparation method of fish collagen peptide compound
  • Preparation method of fish collagen peptide compound
  • Preparation method of fish collagen peptide compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Step 1, fish skin pretreatment:

[0054] Take an appropriate amount of tilapia skin, remove the scales and clean it, soak it in 1mol / L HCl for 24 hours, control the ratio of solid to liquid at 1:10g / ml, and then rinse the skin after acid soaking with clean water to remove the pigment on the surface of the skin. Cut the fish skin into cubes about 1.5×1.5cm in size, soak in 0.1mol / L NaOH solution for 24 hours, control the material-liquid ratio to 1:20g / ml, and stir with a magnetic stirrer at the same time , to remove miscellaneous protein and fat.

[0055] Step 2, prepare collagen solution:

[0056] Soak the pretreated fish skin with an appropriate amount of distilled water for 1 hour to wash away the residual lye. Then soak in citric acid solution with a mass concentration of 1% until the fish skin softens at room temperature, so that the gelatin swells and facilitates the extraction of the gelatin. The ratio of solid to liquid is controlled to be 1:15g / ml. Rinse the ...

Embodiment 2

[0069] Step 1, fish skin pretreatment:

[0070] Take cod skin, descale and clean it, soak it in 0.8mol / L HCl solution for 20 hours, control the ratio of solid to liquid to 1:8g / ml, and then rinse the acid-soaked fish skin with clean water to remove the pigment on the surface of the fish skin. Cut the fish skin into cubes about 1.5×1.5cm in size, soak in 0.15mol / L NaOH solution for 48 hours, control the material-liquid ratio to 1:10g / ml, and stir with a magnetic stirrer at the same time , to remove miscellaneous protein and fat.

[0071] Step 2, prepare collagen solution:

[0072] Soak the pretreated fish skin with appropriate amount of distilled water for 2 hours to wash away the residual lye. Then soak in citric acid solution with a mass concentration of 0.5% at room temperature until the fish skin softens, making the gelatin swell, which is beneficial to gelatin extraction, and the ratio of solid to liquid is controlled to be 1:10g / ml. Rinse the citric acid-treated fish s...

Embodiment 3

[0085] Step 1, fish skin pretreatment:

[0086] Take the grass carp skin, remove the scales and clean it, soak it in 1.2mol / L HCl solution for 26 hours, control the ratio of solid to liquid to 1:9g / ml, and then rinse the acid-soaked fish skin with clean water to remove the pigment on the surface of the fish skin. Cut the fish skin into cubes about 1.5×1.5cm in size, soak in 0.12mol / L NaOH solution for 36 hours, control the material-liquid ratio to 1:15g / ml, and stir with a magnetic stirrer at the same time , to remove miscellaneous protein and fat.

[0087] Step 2, prepare collagen solution:

[0088] Soak the pretreated fish skin with an appropriate amount of distilled water for 1.5 hours to wash away the residual lye. Then soak in a citric acid solution with a mass concentration of 2% at room temperature until the fish skin softens to make the gelatin swell and facilitate gel extraction. The ratio of solid to liquid is controlled to be 1:13g / ml. Rinse the citric acid-treat...

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Abstract

The invention discloses a preparation method of a fish collagen peptide compound. The method includes: processing fish skin with citri acid at 80 to 100 DEG C to obtain collagen, hydrolyzing the collagen with alkaline protease and bromelain, and fermenting hydrolyzed collagen peptides with yeast to obtain the fish collagen peptide compound. By means of a combination of acid extraction and hot water extraction, the collagen is acquired from the fish skin and is then enzymatically hydrolyzed with food industry proteinases and fruit and vegetable juice proteases, microbial fermentation is then enabled to obtain collagen peptides, and the fish collagen peptide compound easy to digest and absorb and of good taste and flavor is prepared. The preparation method is simple and easy to operate and has high collagen extraction rate.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and relates to a preparation method of a fish collagen short peptide complex. Background technique [0002] It has been scientifically proven that the peptides produced by hydrolysis of natural proteins have physiological regulation functions such as immune regulation, hormone regulation, blood pressure lowering, blood fat reduction, anti-fatigue, anti-oxidation, etc. It is a topic of keen research in the current medical and food circles and has great development prospects functional factor. As the main component of animal connective tissue, collagen is the most abundant and widely distributed type of natural protein in animals, and it can be used as one of the main sources for the preparation of collagen peptides. [0003] In recent years, with the outbreak of infectious diseases such as foot-and-mouth disease and mad cow disease, the use of terrestrial animal-derived collagen has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 申铉日刘阳峰
Owner 海南盛美诺生物技术有限公司
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