Chitosan hydrolysis method

A technology of chitosan and dissolving solution, applied in the field of cellulase hydrolysis of chitosan, can solve problems such as low efficiency, and achieve the effects of good water solubility and high yield

Inactive Publication Date: 2015-11-18
CHONGQING TECH & BUSINESS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] One of the purposes of the present invention is to solve the problem of a large number of by-products and low efficiency in the existing method of hydrolyzing chitosan, and then provide a method for hydrolyzing chitosan, the method of the present invention uses cellulase to hydrolyze the shell The polysaccharide product is milky white and has good water solubility, and will not cause pollution to the environment during the production process

Method used

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Embodiment 1

[0027] Example 1 Hydrolysis of Chitosan

[0028] The invention discloses a method for hydrolysis of chitosan, which uses chitosan as raw material, uses binary mixed ionic liquid as solvent of chitosan, uses cellulase as catalyst for hydrolysis, and conducts hydrolysis reaction under constant temperature oscillation reaction conditions.

[0029] The hydrolysis method of above-mentioned chitosan, comprises the steps:

[0030] Step 1: Dissolution of Chitosan

[0031] Dissolve chitin or chitosan in the binary mixed ionic liquid, heat it to 80-130°C under stirring for a period of time until the solution is transparent, the concentration of chitosan in the binary mixed ionic liquid is 5wt%, and cool to room temperature Then add water with 5 times the molar weight of chitosan and cellulase with a weight of 5% of the weight of chitosan, adjust the pH of the solution, and stir at room temperature for 0.5-1 h to obtain a solution of chitosan.

[0032] Step 2: Hydrolysis of Chitosan

...

Embodiment 2

[0034] Example 2 Characterization of Chitosan after Hydrolysis

[0035] The chitosan after enzymatic hydrolysis was centrifuged, and the precipitate was vacuum-dried. A small amount of the product was mixed with potassium bromide and pressed into tablets, and the infrared spectrum was measured by Fourier transform infrared spectrometer. The degree of deacetylation after hydrolysis was analyzed according to the infrared spectrum. At the same time, measure the deacetylation degree of raw chitosan, which is still insoluble in water after enzymatic hydrolysis, and compare it with the infrared image of water-soluble chitosan. The following calculation formula is used:

[0036] A=-logT, where T is the transmittance (%);

[0037] DD=(A 1560 / A 2880 -1.951) / -0.0148×100%;

[0038] In the above formula, A is the absorbance (%); DD is the deacetylation degree (%).

[0039] Figure 1 to Figure 3 Infrared spectra of chitosan before and after cellulase degradation and precipitation o...

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Abstract

The invention discloses a chitosan hydrolysis method and belongs to the technical field of chitosan hydrolysis. In the chitosan hydrolysis method, non-specific cellulase is selected, and chitosan and fibrin are similar in property and structure, so that good hydrolysis effect can be realized due to selection of cellulase. The chitosan hydrolysis method includes: taking chitosan as a raw material; using binary mixed ion liquid as a solvent of chitosan and cellulase as a catalyst for hydrolysis, and performing hydrolysis reaction under a condition of constant-temeprature oscillation, wherein reaction temperature is 45-58 DEG C, a proportion of cellulase and chitosan is 0.05-10%, reaction time is 3-10h, and reaction pH value is 3.6-5.0. The chitosan hydrolysis method has the advantages of being high in efficiency, environment friendly and high yield and has industrialization prospect. The solvent adopted in the method is a green solvent, free of volatilization and recyclable.

Description

technical field [0001] The invention belongs to the technical field of chitosan hydrolysis, and particularly relates to a method for hydrolyzing chitosan with cellulase. Background technique [0002] Chitosan, also known as deacetylated chitin, is obtained by deacetylation of chitin that exists widely in nature. It has many excellent properties and is widely used in medicine, food, environmental protection, agriculture, cosmetics and other fields. Chitosan with small molecular weight has good biological activity and is soluble in water, especially oligomeric chitosan with molecular weight less than 10,000 has unique application functions and physiological activities, such as: good water solubility, antibacterial and antibacterial properties. It has unique application value in biomedicine, health food, medical diagnosis, agriculture and biophysiology, etc. The molecular weight of chitosan is as high as several million, and it is insoluble in water and other solvents, which s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12P19/14
CPCY02P20/54
Inventor 邵承斌王星敏李宁雷文玲
Owner CHONGQING TECH & BUSINESS UNIV
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