Method for preparing squid liver protein ACE (angiotensin converting enzyme) inhibitory peptide by enzymolysis of mixed enzyme

A technology of mixed enzymes and inhibitory peptides, which is applied in the field of preparation of squid liver protein ACE inhibitory peptides by enzymolysis of mixed enzymes, can solve the problems of waste of squid resources and low utilization rate of squid liver processing, and achieve easy absorption by human body, change the waste of squid resources, Effect of waste reduction

Inactive Publication Date: 2015-11-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method for preparing squid liver protein ACE inhibitory peptide by enzymatic hydrolysis with mixed enzymes, which provides more selective ways for the p

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  • Method for preparing squid liver protein ACE (angiotensin converting enzyme) inhibitory peptide by enzymolysis of mixed enzyme

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Embodiment 1

[0021] A method for preparing squid liver protein ACE inhibitory peptide by enzymatic hydrolysis with mixed enzymes, specifically comprising the following steps:

[0022] (1) Pretreatment of squid liver: mince the squid liver and mix it with water at a mass ratio of 1:1, add 6mol / L sodium hydroxide solution at 35°C to adjust the pH to 7, keep it warm for 20min, then raise the temperature to 70°C, React for 1 hour, add sodium chloride accounting for 2% of the liver weight, salt out for 5 minutes, centrifuge at 4000r / min for 15 minutes, remove the upper layer of fish oil, and obtain crude squid liver protein;

[0023] (2) Degreasing of squid liver protein: After dehydrating the crude squid liver protein, add a mixture of n-hexane and isopropanol at a ratio of 1g:3mL to liquid (the volume ratio of n-hexane and isopropanol is 4:3 ), washed 2 times, and vacuum-dried to obtain defatted squid liver protein;

[0024] (3) Mixed enzyme enzymatic hydrolysis: make defatted squid liver pr...

Embodiment 2

[0029] A method for preparing squid liver protein ACE inhibitory peptide by enzymatic hydrolysis with mixed enzymes, specifically comprising the following steps:

[0030] (1) Squid liver pretreatment: mince the squid liver and mix it with water at a mass ratio of 1:1, add 6mol / L sodium hydroxide solution at 40°C to adjust the pH to 9, keep it warm for 30min, then heat up to 90°C, React for 1 hour, add sodium chloride accounting for 3% of the liver weight, salt out for 10 minutes, centrifuge at 8000r / min for 20 minutes, remove the fish oil in the upper layer, and obtain crude squid liver protein;

[0031] (2) Degreasing of squid liver protein: After dehydrating the crude squid liver protein, add a mixture of n-hexane and isopropanol at a ratio of 1g:3mL to liquid (the volume ratio of n-hexane and isopropanol is 4:3 ), washed 3 times, and vacuum-dried to obtain defatted squid liver protein;

[0032] (3) Mixed enzyme enzymatic hydrolysis: make defatted squid liver protein into a...

Embodiment 3

[0038] A method for preparing squid liver protein ACE inhibitory peptide by enzymatic hydrolysis with mixed enzymes, specifically comprising the following steps:

[0039] (1) Pretreatment of squid liver: mince squid liver and mix it with water at a mass ratio of 1:1.5, add 6mol / L sodium hydroxide solution at 50°C to adjust the pH to 8, keep it warm for 25min, and then raise the temperature to 80°C. React for 1.5 hours, add sodium chloride accounting for 4% of the liver weight, salt out for 15 minutes, centrifuge at 10,000 r / min for 30 minutes, remove the upper layer of fish oil, and obtain crude squid liver protein;

[0040] (2) Degreasing of squid liver protein: After dehydrating the crude squid liver protein, add a mixture of n-hexane and isopropanol at a ratio of 1g:3mL to liquid (the volume ratio of n-hexane and isopropanol is 4:3 ), washed 3 times, and vacuum-dried to obtain defatted squid liver protein;

[0041] (3) Enzyme hydrolysis with mixed enzymes: prepare defatted...

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Abstract

The invention discloses a method for preparing a squid liver protein ACE (angiotensin converting enzyme) inhibitory peptide by enzymolysis of a mixed enzyme. The method includes: pretreating squid livers, defatting the squid livers, allowing hydrolysis with the mixed enzyme composed of compound protease, neutral protease and papain, and performing centrifuging and freeze-drying to obtain the squid liver protein ACE inhibitory peptide; preferably, enzymatic hydrolysate is deodorized with Lactobacillus bulgaricus and Lactobacillus helveticus. The squid liver protein ACE inhibitory peptide is good in ACE inhibitory effect; by deodorizing the enzymatic hydrolysate with mixed bacteria, odor of the squid liver protein inhibitory peptide is effectively improved, sensory value of the peptide is improved, and the problem that low utilization rate of existing squid liver processing causes squid resource waste is effectively solved.

Description

technical field [0001] The invention relates to the field of intensive processing of aquatic products, in particular to a method for preparing squid liver protein ACE inhibitory peptide by enzymatic hydrolysis with mixed enzymes. Background technique [0002] Squid is an important marine cephalopod aquatic product, widely distributed in the Atlantic Ocean, Indian Ocean and Pacific Ocean. In recent years, the amount of squid caught in my country has increased year after year, and squid processing has become one of the main components of domestic aquatic product processing. Generally speaking, after the squid is thawed and processed, about 30% of the waste such as head, foot, viscera and epidermis will be produced. From a global perspective, more than 1 million tons of squid catches every year will generate 250,000 to 300,000 tons of squid waste. For these wastes, the general treatment method is to bury them on the spot or process them into fishmeal, which not only wastes fi...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12P1/04C12R1/225
Inventor 谢超林琳
Owner ZHEJIANG OCEAN UNIV
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