Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing quantum dots with nuclear shell structures, fluorescent nanometer probe for target tumor markers GPC-3 and method for preparing fluorescent nanometer probe

A technology of fluorescent nanoprobes and tumor markers, which is applied to the preparation methods of peptides, chemical instruments and methods, preparations for in vivo experiments, etc., can solve the problems of unsafe and environmental protection, and achieve good fluorescent properties, The effect of eliminating biological toxicity and simple steps

Inactive Publication Date: 2015-12-02
NANTONG UNIVERSITY
View PDF9 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of dangerous reagents such as trioctylphosphine (TOP) or tributylphosphate (TBP) in the synthesis of core-shell quantum dots is neither safe nor environmentally friendly.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing quantum dots with nuclear shell structures, fluorescent nanometer probe for target tumor markers GPC-3 and method for preparing fluorescent nanometer probe
  • Method for preparing quantum dots with nuclear shell structures, fluorescent nanometer probe for target tumor markers GPC-3 and method for preparing fluorescent nanometer probe
  • Method for preparing quantum dots with nuclear shell structures, fluorescent nanometer probe for target tumor markers GPC-3 and method for preparing fluorescent nanometer probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing ZnO / ZnS core-shell structure quantum dots, by co-precipitation method, mixing zinc acetate and sodium acetate mixture, heating and oxidizing to prepare ZnO, and then adding thioacetamide as S 2- According to S-Zn-S-Zn, it is coated with ZnS shell layer by layer to form ZnO / ZnS quantum dots.

[0038] The concrete steps of above-mentioned preparation method are as follows:

[0039] (101-1) Add 3.5g of zinc acetate and 0.4g of sodium acetate into a round bottom flask filled with 200mL of ethanol;

[0040] (101-2) After heating to dissolve, quickly add 19.5mL of 1.0mol / l sodium oxide ethanol solution, react for 30 minutes, and cool to room temperature;

[0041] (101-3) Add excess ethyl acetate dropwise to the solution at a rate of 1 drop / second until precipitation occurs, centrifuge to separate the precipitate, wash with ethyl acetate for 3 to 5 times, and then redisperse the precipitate in In ethanol, make ZnO nanoparticles;

[0042] (101-4) Add 3...

Embodiment 2

[0046] A fluorescent nanoprobe targeting the tumor marker GPC-3 is prepared by surface-modified monoclonal antibody GC-33 with ZnS as the carrier and the core-shell fluorescent quantum dots as the carrier. The schematic diagram of the process is as follows: figure 2 shown.

Embodiment 3

[0048] A method for preparing a fluorescent nanoprobe targeting tumor marker GPC-3, comprising the steps of:

[0049] (102-1) Prepare ZnO / ZnS core-shell quantum dots according to the method provided in Example 1;

[0050] (102-2) Take 5ml of ZnO / ZnS core-shell structure quantum dot solution for super-dispersion, centrifuge at 15000rpm for 30min, add 0.5ml of ultrapure water, and set aside;

[0051] (102-3) Dissolve 10mg of carbodiimide (EDC), 2mg of N-hydroxysulfosuccinimide (NHSS) in 1.0ml of water;

[0052] (102-4) Take 0.5ml of the above solution and add it to the ZnO / ZnS core-shell structure quantum dot solution, set the solution to 1.0ml, and react on a shaking table at 37°C for 1 hour;

[0053] (102-5) Centrifuge at 15,000 rpm for 30 minutes at high speed, discard the supernatant, and redissolve with 250 μl of PBS solution with pH 8.5;

[0054] (102-6) Add 5 μl of GPC-3-targeting monoclonal antibody GC-33 solution, and react on a shaking table at a constant temperature...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing quantum dots with nuclear shell structures, a fluorescent nanometer probe for target tumor markers GPC-3 and a method for preparing the fluorescent nanometer probe. The method for preparing the fluorescent nanometer probe includes preparing the quantum dots with the nuclear shell structures and ZnS as shells; activating carboxyl on the surfaces of the quantum dots with the nuclear shell structures and the ZnS as the shells by the aid of carbodiimide (EDC) and N-hydroxy succinimide (NHS); adding monoclonal antibodies GC33 to the quantum dots to enable the monoclonal antibodies and the activated carboxyl on the surfaces of the quantum dots with the nuclear shell structures and the ZnS as the shells to form amido bonds; modifying the surfaces of the quantum dots by the aid of the monoclonal antibodies GC33 (IgG2a, k) to obtain the nanometer probe (GC-QDs). The methods and the fluorescent nanometer probe have the advantages that the surfaces of the quantum dots are modified by the monoclonal antibodies GC-33 to obtain the nanometer probe with high specificity and high target ability, influence of biological tissues on fluorescent interference can be overcome, accordingly, in-situ and in-vivo labeling and imaging on liver cancer can be implemented, and experimental bases and theoretical research can be provided for clinical application of the fluorescent quantum dots.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and in particular relates to a method for preparing quantum dots with a core-shell structure, a fluorescent nanoprobe targeting tumor marker GPC-3 and a method for preparing the same. Using ZnS as the shell layer, a series of nanometer probes are designed and synthesized. Fluorescent quantum dots with a core-shell structure can be made into fluorescent nanoprobes (GC-QDs) targeting liver cancer cell markers after being coupled to the antibody GC33 through physical adsorption, which can be used in biomarkers and drug carrier systems. Background technique [0002] Quantum dots (quantumdots; QDs) are nanoparticles composed of II-VI and III-V elements, which have excellent fluorescent properties. Compared with ordinary organic fluorescent dyes, fluorescent quantum dots have a wide excitation wavelength range and narrow and symmetrical It can be used as an ideal fluorescent probe and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/56C07K16/30C07K1/13A61K49/00A61K9/51A61K47/04
Inventor 李景源姚登福邵义祥朱顺星王生存
Owner NANTONG UNIVERSITY