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Trichoderma voglmayrii producing mycelium cultivation medium, method for preparing same and application

A technology of mycelium culture medium and culture method, which is applied in the field of mycelia culture medium and its preparation of Trichoderma vogma, which can solve the problems of slow growth, less aerial hyphae, and less spore production of Trichoderma vogma , to achieve the effects of easy separation and extraction, fast bacterial growth and high mycelium yield

Inactive Publication Date: 2015-12-02
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a medium for Trichoderma vugma and its preparation method and application, which solves the problems of slow growth, few aerial hyphae, less spore production and long time in other culture media for Trichoderma vugma

Method used

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  • Trichoderma voglmayrii producing mycelium cultivation medium, method for preparing same and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Utilizes the mycelia-producing medium to ferment and produce Trichoderma vogemar mycelium

[0022] The formula of Trichoderma Vogma mycelium production medium is: dextrin 50g, peptone 21g, yeast powder 2.9g, magnesium sulfate 2.3g, potassium dihydrogen phosphate 0.9g, add water to 1000mL, and the pH is natural.

[0023] Control medium 1 (GaoHJ, ChuX, WangYW, ZhouF, ZhaoK, MuZM, LiuQX. 2013. Media optimization for lac case production by Trichoderma harzianum ZF-2 using response surface methodology. J. Microbiol. Biotechnol. 23: 1757–1764.): 20 g of glucose, 1 g of ammonium sulfate, potassium dihydrogen phosphate 0.6g, magnesium sulfate 1g, add water to 1000mL, pH natural.

[0024] Control medium 2: 200g potatoes, 20g glucose, add water to 1000mL, natural pH.

[0025] Trichodermavoglmayrii strain (Trichodermavoglmayrii) P-T8196 (the inventor of this patent application was isolated and deposited in the China General Microorganism Culture Collection and Manage...

Embodiment 2

[0031] Embodiment 2 Utilizes the mycelia-producing medium to ferment and produce Trichoderma vogemar mycelium

[0032] The formula of Trichoderma Vogma mycelium production medium is: dextrin 40g, peptone 20g, yeast powder 3g, magnesium sulfate 1g, potassium dihydrogen phosphate 0.8g, add water to 1000mL, pH is natural.

[0033] Control medium 1 (Gao et al. 2013): glucose 20g, ammonium sulfate 1g, potassium dihydrogen phosphate 0.6g, magnesium sulfate 1g, add water to 1000mL, pH natural.

[0034] Control medium 2: 200g potatoes, 20g glucose, add water to 1000mL, natural pH.

[0035] Trichoderma vogma strain P-T8196 (separated and preserved by the inventor of this patent application) was inoculated on potato dextrose solid medium (PDA) for activation, cultured at 25°C for 3 days, and then the diameter was taken at the edge of the colony with a puncher 8 bacterial blocks of 5mm were inoculated in potato dextrose medium (PDB) with a liquid volume of 100mL (250mL Erlenmeyer flask)...

Embodiment 3

[0038] Embodiment 3 Utilizes the mycelia-producing medium to ferment to produce Trichoderma vogemar mycelium

[0039] The formula of Trichoderma Vogma mycelia production medium is: dextrin 60g, peptone 20g, yeast powder 5g, magnesium sulfate 1g, potassium dihydrogen phosphate 0.8g, add water to 1000mL, pH is natural.

[0040] Control medium 1 (Gao et al. 2013): glucose 20g, ammonium sulfate 1g, potassium dihydrogen phosphate 0.6g, magnesium sulfate 1g, add water to 1000mL, pH natural.

[0041]Control medium 2: 200g potatoes, 20g glucose, add water to 1000mL, natural pH.

[0042] Trichoderma vogma strain P-T8196 (separated and preserved by the inventor of this patent application) was inoculated on potato dextrose solid medium (PDA) for activation, cultured at 25°C for 3 days, and then the diameter was taken at the edge of the colony with a puncher 8 bacterial blocks of 5mm were inoculated in potato dextrose medium (PDB) with a liquid volume of 100mL (250mL Erlenmeyer flask), p...

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Abstract

The invention provides a trichoderma voglmayrii producing mycelium cultivation medium. The cultivation medium particularly comprises, by weight, 20-60g of dextrin, 10-30g of peptone, 1-5g of yeast powder, 0.5-4.5g of magnesium sulfate, 0.6-1g of monopotassium phosphate, 1000mL of water and pH (potential of hydrogen) nature. The trichoderma voglmayrii producing mycelium cultivation medium has the advantages that the cultivation medium for fermenting and producing trichoderma voglmayrii is simple in process and high in thallus growth speed and mycelium yield; carbon and nitrogen sources in the cultivation medium are not introduced into agricultural and sideline products, so that metabolic products are easy to separate and extract, and the trichoderma voglmayrii producing mycelium cultivation medium is suitable for theoretical research on the trichoderma voglmayrii and industrial fermentation production.

Description

technical field [0001] The invention relates to the field of microorganism culture, and more specifically, to a mycelia-producing culture medium of Trichoderma vogemarum and its preparation method and application. Background technique [0002] There are many species of Trichoderma fungi, which are widely distributed and are an important biological resource. Some species play an important role in many fields and have good economic value and application prospects, so they have attracted much attention. [0003] In 2005, Trichodermavoglmayrii (Trichodermavoglmayrii) was first reported in Austria. In 2014, the inventor of this patent application first discovered the distribution of this species in China, and screened and proved that Trichodermavoglmayrii P-T8196 strain is effective against various plant pathogens. Fungi (hereinafter referred to as plant pathogens) all have certain biocontrol potential. However, the bacterial strain grows slowly on multiple mediums such as PDA, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/885
Inventor 庄文颖秦文韬朱兆香宋霞
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI