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Extraction method of collagen fiber

A technology of collagen fiber and extraction method, which is applied in the field of extraction of collagen fiber and collagen fiber, and can solve the problems of fast degradation rate and too small fiber diameter

Inactive Publication Date: 2015-12-23
MICONVEY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a method for extracting collagen fibers, which is used to solve the problem in the prior art that the fiber diameter is too small to cause the degradation rate to be too fast, and has relatively high Collagen fibers in animal Achilles tendon with high purity and effective virus removal

Method used

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  • Extraction method of collagen fiber
  • Extraction method of collagen fiber
  • Extraction method of collagen fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Clean the fresh beef Achilles tendon with purified water, remove the fascia and lean meat with a scalpel blade, cut the beef Achilles tendon, and cut it into 0.5mm thick Achilles tendon slices.

[0084] Take 100 g of Achilles tendon slices and soak them in 5 L of 0.01 mol / L NaOH aqueous solution at 10° C. for 24 hours, wash them with purified water, and adjust the pH value to 7. The Achilles tendon piece was added to 5 L of 0.01 mol / L hydrochloric acid solution and soaked at 10° C. for 48 hours, then washed with purified water, and the pH value was adjusted to 7. Disperse the cleaned tissue in 5L of purified water, adjust the pH to 8.2, add 2g of trypsin to the purified water and treat at 37°C for 2h, then wash with purified water, and adjust the pH to 7.

[0085] The treated Achilles tendon slices were dissolved in 5 L of acetic acid solution with a concentration of 0.3 mol / L, and then pulverized with a tissue grinder. Then add 1 g of pepsin and perform enzymatic hydr...

Embodiment 2

[0090] Clean the fresh beef Achilles tendon with purified water, remove the fascia and lean meat with a scalpel blade, cut the beef Achilles tendon, and cut it into Achilles tendon slices with a thickness of 5 mm.

[0091] Take 100 g of Achilles tendon slices and soak them in 10 L of 0.01 mol / L NaOH aqueous solution at 2° C. for 12 hours, wash with purified water, and adjust the pH value to 7. The Achilles tendon piece was added to 10 L of 0.01 mol / L hydrochloric acid solution and soaked at 2° C. for 12 hours, then washed with purified water, and the pH value was adjusted to 7. Disperse the cleaned tissue in 10 L of purified water, adjust the pH to 8.0, add 3 g of trypsin to the purified water and treat at 35°C for 3 hours, then wash with purified water, and adjust the pH to 7.

[0092] The treated Achilles tendon slices were dissolved in 20 L of acetic acid solution with a concentration of 0.01 mol / L, and then pulverized with a tissue grinder. Add 2 g of pepsin and perform e...

Embodiment 3

[0103] The collagen fiber samples extracted in Example 1 and Comparative Example 1 were observed with a scanning electron microscope respectively, and the specific data of the fiber diameters can be found in figure 1 , figure 2 . Depend on figure 1 , 2 It can be seen that the average fiber diameter of collagen fiber sample 1# in Example 1 is greater than or equal to 10 μm, while the average fiber diameter of collagen fiber sample 1* in Comparative Example 1 is less than 3 μm. It can be seen that the fiber diameter of the sample in Example 1 is much larger than that in Comparative Example 1.

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Abstract

The invention provides an extraction method of collagen fiber. Through selection of the optimum technological steps and conditions, the collagen fiber is extracted from animal achilles tendon and is sterilized by being soaked in alkaline water and an acid solution, impure protein is removed through trypsin processing, and the collagen fiber which has higher purity, doesn't have viruses and has a larger fiber diameter is obtained. The extracted collagen fiber has excellent biological characteristics of better biological activity and the like and can be applied to the fields of cosmetics and biological materials, for example, researches of a hemostatic material and artificial skin and tissue filler such as cartilage, mucosal tissue, bone tissue and the like.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to a method for extracting collagen fibers, in particular to a method for extracting collagen fibers from animal Achilles tendons. Background technique [0002] Collagen is the most widely distributed and most abundant structural protein in multicellular animals. It often has a fibrous structure. It is widely present in all tissues from the body surface of lower spinal cord animals to mammalian organisms. It is the content in mammals The largest type of protein, accounting for 25%-30% of the total protein content. Collagen fibers have a triple helix structure, which is related to the formation, maturation, and transmission of information between cells, cell proliferation, differentiation, movement, cellular immunity, tumor metastasis, and joint lubrication, wound healing, calcification, blood coagulation, and aging. It is also closely related to the occurrence of many connective...

Claims

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Application Information

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IPC IPC(8): C12P21/06D01F4/00
Inventor 李康郑宗清
Owner MICONVEY TECH CO LTD
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