Carthamus tinctorius L. extract, cosmetic adopting same as active ingredient and application

A safflower extract and extract technology are applied in the directions of cosmetics, medical preparations containing active ingredients, cosmetic preparations, etc., which can solve the problem of no safflower extract, and achieve good tyrosinase activity inhibition. effect, widening the way of use, improving the effect of use function and added value

Inactive Publication Date: 2015-12-30
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although humans have a long history of using safflower, there are no report

Method used

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  • Carthamus tinctorius L. extract, cosmetic adopting same as active ingredient and application
  • Carthamus tinctorius L. extract, cosmetic adopting same as active ingredient and application
  • Carthamus tinctorius L. extract, cosmetic adopting same as active ingredient and application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1:

[0023] Preparation of safflower extract:

[0024] Weigh 60g of safflower (Carthamustinctorius L.) sample (safflower bee pollen or safflower inflorescence), grind the sample into powder, cold soak it with 25 times the amount of 85% ethanol (1807-1808mL) for 24h, and then collect it with a Buchner funnel Filtrate, repeat 3 times. The filtrate was distilled under reduced pressure with a rotary evaporator, the filtrate was concentrated, and the concentrated filtrate was dried at low temperature to obtain a crude extract. The crude extract was adsorbed and decolorized by macroporous resin (D101). After elution with 95% ethanol, the eluate was concentrated and evaporated to dryness and freeze-dried at low temperature to obtain 18.6 g of safflower inflorescence extract with an extraction rate of 31%; Bee pollen extract 34.6g, the extraction rate is 57.6%;

[0025] Alternatively, weigh 60g of Carthamustinctorius L. sample (cartasmanus bee pollen or safflower infloresce...

Example Embodiment

[0028] Example 2:

[0029] Cytotoxicity test of safflower extract: Determine the CC of the sample by MTS colorimetry 50 (50%cytotoxicconcentration), that is, the concentration of the drug when it is toxic to 50% of the cells, so as to determine the safe sample concentration for the following activity experiment.

[0030] Experimental method: On a 96-well cell culture plate, mix B16 cells with different concentrations of the drug solution to be tested, set 3 replicate wells, and set a blank control without drug at 37°C, 5% CO 2 Cultivate for 24h, use MTS colorimetric method to detect cytotoxicity, measure OD value with microplate reader, and measure the wavelength at 490nm. Calculate CC 50 value.

[0031] Cell survival rate (%) = OD of experimental well 490nm / Blank hole OD 490nm ×100%

[0032] Experimental results: The cell survival rate of safflower inflorescence extract was 118.877%, the cell survival rate of safflower bee pollen extract was 111.418%, and the cell survival rate of...

Example Embodiment

[0033] Example 3:

[0034] Tyrosinase inhibition experiment: mix the drug to be tested with L-Dopa (final concentration 1.25mM), add tyrosinase (final concentration 25U / mL) to start the reaction, set 3 repeat wells, and set no drug at the same time The blank control and KojicAcid positive control, room temperature, 5min, OD value measured by microplate reader, detection wavelength is 490nm. Calculate the inhibition rate of tyrosinase activity.

[0035] Inhibition rate of tyrosinase activity (%) = [1-(sample OD 490nm -Sample background control OD 490nm ) / Experimental control well OD 490nm 〕×100%

[0036] Experimental results: safflower inflorescence extract and safflower bee pollen extract have a good inhibitory effect on tyrosinase (see figure 2 , 3 ), compare the extract concentration with 50% inhibition rate. The safflower pollen extract is IC 50 =89.231μg / mL, safflower inflorescence extract is IC 50 = 113.176μg / mL, indicating that safflower bee pollen and safflower infloresc...

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Abstract

The invention discloses Carthamus tinctorius L. plant extract, a cosmetic additive adopting the same for realizing skin whitening, moisturizing and antioxidant activity and a combined application of the Carthamus tinctorius L. extract in cosmetics. The Carthamus tinctorius L. extract is ethanol extract of Carthamus tinctorius L. bee pollen or Carthamus tinctorius L. inflorescence or Carthamus tinctorius L. seed oil extract. The extract has good DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical cleaning capacity and is a good natural antioxidant. The Carthamus tinctorius L. extract has excellent skin cosmetic application potential in aspects of wrinkle resisting, whitening, sunscreen and the like in terms of skin care of a human body. With the effective utilization of Carthamus tinctorius L. which is the traditional medicinal plant in China, the Carthamus tinctorius L. extract with very high skin cosmetic activity is obtained, and the extract can be used for preparing a good tyrosinase activity inhibitor and a collagen secretion promoting active agent and is a good raw material for herbal cosmetics.

Description

technical field [0001] The invention belongs to the technical field of cosmetics and medicine, and in particular relates to extracting a natural active substance from a traditional Chinese medicinal plant safflower—safflower extract, cosmetics or medicines using it as an active ingredient, and the preparation of safflower extract Applications in various skin cosmetics such as moisturizing, skin rejuvenation, anti-wrinkle, sunscreen, and whitening, as well as in the preparation of drugs for the treatment or prevention of skin diseases technical background [0002] Safflower (Carthamustinctorius L.), also known as red orchid and thorny safflower, is a plant of the genus Safflower in the family Asteraceae. It has dry tubular flowers, orange-red, with a specific aroma and a slightly bitter taste. Safflower is mainly distributed in areas with warm and dry climate. In my country, it is common in Henan, Hunan, Sichuan, Xinjiang and Tibet. [0003] Common uses of safflower are medi...

Claims

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Application Information

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IPC IPC(8): A61K36/286A61P17/00A61K8/97A61Q19/00A61Q19/02A61Q19/08A61Q17/04
Inventor 杨立新许刚刘爱忠裴盛基
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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