Magnetic whole-cell catalyst, preparation method and method for producing biodiesel

Abstract

The invention discloses a magnetic whole-cell catalyst, a preparation method and a method for producing biodiesel. The preparation method includes the steps that firstly, pseudomonas mendocina cells are cultured and collected; secondly, SiO2-Fe3O4 nanoparticles are taken and added into a sodium alginate solution, the bacterial cells obtained in the first step are added after uniform stirring, and a mixed solution is obtained after uniform mixing; thirdly, the mixed solution is added into a sterile CaCl2 aqueous solution drop by drop under the stirring condition, still standing is carried out, magnetic microspheres are attracted out through a magnet and cleaned, and the magnetic whole-cell catalyst is obtained. The particle diameter of the prepared magnetic whole-cell catalyst is 2-6 mm, biological activity is high, and stability is good. In a biodiesel preparation reaction system, the magnetic whole-cell catalyst can be directly separated and extracted through the magnet and repeatedly used. The catalyst is easy to recycle, stable, easy to operate, moderate in condition, high in adaptability and safety and capable of reducing production cost of the biodiesel.

Description

technical field

[0001] The invention relates to the fields of biology and energy chemical industry, in particular to a method for producing biodiesel with a magnetic whole-cell catalyst. Background technique

[0002] As a clean renewable energy, the development of biodiesel has broad market prospects. The preparation of biodiesel mainly adopts chemical method, which has the advantages of simple process, low operating cost and stable properties of the obtained product. However, the chemical method has the following disadvantages: high reaction temperature, complicated process; high energy consumption; complicated product purification, difficult recovery of esterified products; difficult removal of by-products; easy generation of waste water, causing secondary pollution to the environment, etc.

[0003] In order to solve the above-mentioned problems existing in the chemical method, people began to study in depth the preparation of biodiesel by using the biological method. A ...

Examples

Embodiment 1

[0031] A method for preparing a magnetic whole-cell catalyst, comprising the steps of:

[0032] (1) Inoculate Pseudomonas mendocina (hereinafter referred to as Pseudomonas mendocina) with the preservation number of CGMCCNo.7644 into 100mLLB medium, cultivate it at 35°C and 160r / min for 20h, and then cultivate it at 9000r / min Centrifuge for 12 minutes, discard the supernatant, and collect Pseudomonas mendoza cells;

[0033] (2) Proportionally, take 1gSiO 2 -Fe 3 o 4 The nanoparticles are added to 70mL of 3% sodium alginate solution by mass percentage, stirred evenly, and then the bacterial cells obtained in step (1) are added, and the mixed solution is obtained after mixing; the solvent of the sodium alginate solution is 1M Sodium hydroxide aqueous solution;

[0034] (3) The mixed solution obtained in step (2) is dripped dropwise into 150 mL of 1.5 mol / L sterile CaCl under stirring conditions with a syringe with an inner diameter of 1.36 mm. 2 In the aqueous solution, the ...

Embodiment 2

[0036] A method for preparing a magnetic whole-cell catalyst, comprising the steps of:

[0037] (1) Inoculate Pseudomonas mendoza into 125mL LB medium, culture at 30°C and 150r / min for 24h, centrifuge at 7000r / min for 15min, discard the supernatant, and collect Pseudomonas mendoza cells;

[0038] (2) Proportionally, take 0.5gSiO2 -Fe 3 o 4 Add nanoparticles into 50mL of 2.5% sodium alginate solution by mass percentage, stir evenly, then add the bacterial cells obtained in step (1), and mix to obtain a mixed solution; the solvent of the sodium alginate solution is 0.5% M sodium hydroxide aqueous solution;

[0039] (3) The internal diameter of the dropper that the mixed solution that step (2) obtains is 1.19mm is dripped dropwise into 180mL1mol / L sterile CaCl under stirring condition 2 In the aqueous solution, the stirring rate is 200r / min. After the dropwise addition of the mixed solution is completed, let it stand for 3 hours, suck out the magnetic microspheres with a magne...

Embodiment 3

[0043] A method for preparing a magnetic whole-cell catalyst, comprising the steps of:

[0044] (1) Inoculate Pseudomonas mendoza into 150mL LB medium, culture at 28°C and 140r / min for 30h, centrifuge at 10000r / min for 10min, discard the supernatant, and collect Pseudomonas mendoza cells;

[0045] (2) Proportionally, take 1.5gSiO 2 -Fe 3 o 4 Add nanoparticles into 100mL of 3.5% sodium alginate solution by mass, stir evenly, then add the bacterial cells obtained in step (1), and mix to obtain a mixed solution; the solvent of the sodium alginate solution is 1.5% M sodium hydroxide aqueous solution;

[0046] (3) The mixed solution obtained in step (2) is dripped dropwise into 200mL2mol / L sterile CaCl under stirring conditions with a syringe with an inner diameter of 1.54mm. 2 In the aqueous solution, the rate of stirring is 400r / min. After the dropwise addition of the mixed solution is completed, let it stand for 1.5h, suck out the magnetic microspheres with a magnet, and was...

technical field

[0001] The invention relates to the fields of biology and energy chemical industry, in particular to a method for producing biodiesel with a magnetic whole-cell catalyst. Background technique

[0002] As a clean renewable energy, the development of biodiesel has broad market prospects. The preparation of biodiesel mainly adopts chemical method, which has the advantages of simple process, low operating cost and stable properties of the obtained product. However, the chemical method has the following disadvantages: high reaction temperature, complicated process; high energy consumption; complicated product purification, difficult recovery of esterified products; difficult removal of by-products; easy generation of waste water, causing secondary pollution to the environment, etc.

[0003] In order to solve the above-mentioned problems existing in the chemical method, people began to study in depth the preparation of biodiesel by using the biological method. A ...

Examples

Embodiment 1

[0031] A method for preparing a magnetic whole-cell catalyst, comprising the steps of:

[0032] (1) Inoculate Pseudomonas mendocina (hereinafter referred to as Pseudomonas mendocina) with the preservation number of CGMCCNo.7644 into 100mLLB medium, cultivate it at 35°C and 160r / min for 20h, and then cultivate it at 9000r / min Centrifuge for 12 minutes, discard the supernatant, and collect Pseudomonas mendoza cells;

[0033] (2) Proportionally, take 1gSiO 2 -Fe 3 o 4 The nanoparticles are added to 70mL of 3% sodium alginate solution by mass percentage, stirred evenly, and then the bacterial cells obtained in step (1) are added, and the mixed solution is obtained after mixing; the solvent of the sodium alginate solution is 1M Sodium hydroxide aqueous solution;

[0034] (3) The mixed solution obtained in step (2) is dripped dropwise into 150 mL of 1.5 mol / L sterile CaCl under stirring conditions with a syringe with an inner diameter of 1.36 mm. 2 In the aqueous solution, the ...

Embodiment 2

[0036] A method for preparing a magnetic whole-cell catalyst, comprising the steps of:

[0037] (1) Inoculate Pseudomonas mendoza into 125mL LB medium, culture at 30°C and 150r / min for 24h, centrifuge at 7000r / min for 15min, discard the supernatant, and collect Pseudomonas mendoza cells;

[0038] (2) Proportionally, take 0.5gSiO2 -Fe 3 o 4 Add nanoparticles into 50mL of 2.5% sodium alginate solution by mass percentage, stir evenly, then add the bacterial cells obtained in step (1), and mix to obtain a mixed solution; the solvent of the sodium alginate solution is 0.5% M sodium hydroxide aqueous solution;

[0039] (3) The internal diameter of the dropper that the mixed solution that step (2) obtains is 1.19mm is dripped dropwise into 180mL1mol / L sterile CaCl under stirring condition 2 In the aqueous solution, the stirring rate is 200r / min. After the dropwise addition of the mixed solution is completed, let it stand for 3 hours, suck out the magnetic microspheres with a magne...

Embodiment 3

[0043] A method for preparing a magnetic whole-cell catalyst, comprising the steps of:

[0044] (1) Inoculate Pseudomonas mendoza into 150mL LB medium, culture at 28°C and 140r / min for 30h, centrifuge at 10000r / min for 10min, discard the supernatant, and collect Pseudomonas mendoza cells;

[0045] (2) Proportionally, take 1.5gSiO 2 -Fe 3 o 4 Add nanoparticles into 100mL of 3.5% sodium alginate solution by mass, stir evenly, then add the bacterial cells obtained in step (1), and mix to obtain a mixed solution; the solvent of the sodium alginate solution is 1.5% M sodium hydroxide aqueous solution;

[0046] (3) The mixed solution obtained in step (2) is dripped dropwise into 200mL2mol / L sterile CaCl under stirring conditions with a syringe with an inner diameter of 1.54mm. 2 In the aqueous solution, the rate of stirring is 400r / min. After the dropwise addition of the mixed solution is completed, let it stand for 1.5h, suck out the magnetic microspheres with a magnet, and was...

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