Magnetic whole-cell catalyst, preparation method and method for producing biodiesel

Abstract

The invention discloses a magnetic whole-cell catalyst, a preparation method and a method for producing biodiesel. The preparation method includes the steps that firstly, pseudomonas mendocina cells are cultured and collected; secondly, SiO2-Fe3O4 nanoparticles are taken and added into a sodium alginate solution, the bacterial cells obtained in the first step are added after uniform stirring, and a mixed solution is obtained after uniform mixing; thirdly, the mixed solution is added into a sterile CaCl2 aqueous solution drop by drop under the stirring condition, still standing is carried out, magnetic microspheres are attracted out through a magnet and cleaned, and the magnetic whole-cell catalyst is obtained. The particle diameter of the prepared magnetic whole-cell catalyst is 2-6 mm, biological activity is high, and stability is good. In a biodiesel preparation reaction system, the magnetic whole-cell catalyst can be directly separated and extracted through the magnet and repeatedly used. The catalyst is easy to recycle, stable, easy to operate, moderate in condition, high in adaptability and safety and capable of reducing production cost of the biodiesel.

Description

technical field [0001] The invention relates to the fields of biology and energy chemical industry, in particular to a method for producing biodiesel with a magnetic whole-cell catalyst. Background technique [0002] As a clean renewable energy, the development of biodiesel has broad market prospects. The preparation of biodiesel mainly adopts chemical method, which has the advantages of simple process, low operating cost and stable properties of the obtained product. However, the chemical method has the following disadvantages: high reaction temperature, complicated process; high energy consumption; complicated product purification, difficult recovery of esterified products; difficult removal of by-products; easy generation of waste water, causing secondary pollution to the environment, etc. [0003] In order to solve the above-mentioned problems existing in the chemical method, people began to study in depth the preparation of biodiesel by using the biological method. A ...

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Problems solved by technology

However, the chemical method has the following disadvantages: high reaction temperature, complex process; high energy consumption; complicated product purification, difficult recovery of esterification products; difficult removal of by-products; easy to produce waste water, causing secondary pollution to the environment, etc.

However, these methods of using whole-cell catalysts to prepare biodiesel have a complicated operation process, and the cells are not immobilized, so it is difficult to separate the whole-cell catalysts in the biodiesel preparation reaction system.

[0004] Chinese patent CN102952754A discloses a method of using immobilized cells to prepare biodiesel. The method is divided into two steps. First, the porous polyaminated chitosan microsphere carrier is prepared, and then the self-made microbial bacteria with high lipase activity units are species, immobilized on the microsphere carrier, and then catalyzed waste cooking oil to prepare biodiesel, the method is complicated to operate,

Chinese patent CN103421716A discloses a preparation method of a magnetic whole-cell catalyst, which is also divided into two steps. First, magnetic chitosan microspheres are prepared, and then fat-producing bacteria are fixed on the microsphere carrier. The preparation process is more complicated and coagulated. The solution uses 20% NaOH aqueous solution by mass percentage and 30% formaldehyde aqueous solution by volume percentage. High-concentration NaOH aqueous solution is very corrosive to the container, and formaldehyde is poisonous, causing environmental pollution and low safety.

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