Temperature sensitive cell culture surface and method for making same

Abstract

The invention relates to a temperature-sensitive cell culture surface and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a temperature-sensitive stock solution: adding a temperature-sensitive compound and a free radical initiator into a solvent, stirring and dissolving to obtain a temperature-sensitive stock solution, and the mass ratio of the temperature-sensitive compound to the solvent is (2-50): 100, the molar ratio of the free radical initiator to the temperature-sensitive compound is (0.5-50): 100; (2) after the temperature-sensitive stock solution is distributed on the cell culture surface, the Place the cell culture surface at 50-150°C and react for 5-120 minutes. The production method uses ordinary chemical free radical initiators to directly trigger the graft polymerization of temperature-sensitive compounds on the surface of cell culture, so that a cell culture surface with temperature-sensitive response function can be obtained, and the temperature sensitivity is greatly reduced compared with the prior art. The production cost of the type cell culture surface is high, the grafting rate is high, and the environment is friendly.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a temperature-sensitive cell culture surface and a preparation method thereof. Background technique [0002] In recent years, the technology of tissue regeneration engineering, biomedical engineering, and cell therapy industry has developed rapidly. It has become the current cell clinical practice to obtain a large number of cells, cell extracellular secretions, and cell therapy products through large-scale culture of animal or human cells in vitro. Important direction of application. For example, tissue trauma, skin cell culture in burn treatment and acquisition of new skin, tumor cell therapy, cell beauty, cell weight loss, etc., all require in vitro culture of target cells. [0003] Cells are mainly divided into suspension cells and adherent cells according to their in vitro culture characteristics. Suspension cells are suspended in the culture medium in the culture devi...

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Problems solved by technology

[0005] In the existing methods for preparing temperature-sensitive cell culture surfaces, some methods are used to physically coat temperature-sensitive compounds directly on the surface of cell culture devices. The temperature-sensitive response function achieved by this method is unstable. Sensitive compounds will fall off, so the effect of automatic cell detachment cannot be guaranteed; most of the others use high-energy electron beams to initiate the grafting of temperature-sensitive compounds on the cell culture surface, and a small number of them use ultraviolet light irradiation to initiate grafting, or plasma-induced grafting , or atom transfer radicals to initiate grafting, in these ways of realizing the grafting of surface temperature-sensitive compounds, some production equipment is expensive (such as high-energy electron beam, plasma), and some processes are complex and the cost of required raw materials is high, which is not conducive to the industry. (such as atom transfer radical initiation, ultraviolet light irradiation initiation), and some grafting efficiency is low, serious environmental pollution (such as plasma)

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