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Construction method and detection method of potassium ion nucleic acid aptamer photoelectrochemical sensor based on diluted magnetic semiconductor

A nucleic acid aptamer and dilute magnetic semiconductor technology, applied in the field of chemical analysis and testing, to achieve the effects of improving sensitivity, simple equipment and high efficiency

Active Publication Date: 2016-01-13
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there have been no reports of photoelectrochemical detection methods based on dilute magnetic semiconductor potassium ion nucleic acid aptamers, and the construction and detection methods of potassium ion nucleic acid aptamer-based photoelectrochemical sensors are still blank in this field.

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  • Construction method and detection method of potassium ion nucleic acid aptamer photoelectrochemical sensor based on diluted magnetic semiconductor
  • Construction method and detection method of potassium ion nucleic acid aptamer photoelectrochemical sensor based on diluted magnetic semiconductor
  • Construction method and detection method of potassium ion nucleic acid aptamer photoelectrochemical sensor based on diluted magnetic semiconductor

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Embodiment 1

[0034] Embodiment 1: A method for constructing a potassium ion nucleic acid aptamer photoelectrochemical sensor based on a dilute magnetic semiconductor, comprising the following steps:

[0035] (1) Use a mixed solution of ammonia, hydrogen peroxide and water with a volume ratio of 1:1:50 to clean the ITO conductive glass electrode, then rinse it with water and dry it in the air for use;

[0036] (2) The ITO conductive glass electrode after drying in the step (1) is immersed in the mixed solution of zinc nitrate, potassium nitrate and manganese sulfate to carry out electrochemical deposition, relative to the deposition potential of silver / silver chloride electrode is-1.0V , the deposition temperature is 80°C, and the deposition time is 120min. After deposition, the Mn-doped dendritic zinc oxide nanorod photoanode is obtained, and the concentration of zinc nitrate solution in the mixed solution is 0.5mmolL -1 , the concentration of potassium nitrate solution is 0.1molL -1 , th...

Embodiment 2

[0054] Embodiment 2: A construction method of a potassium ion nucleic acid aptamer photoelectrochemical sensor based on a dilute magnetic semiconductor, comprising the following steps:

[0055] (1) Use a mixed solution of ammonia, hydrogen peroxide and water with a volume ratio of 1:1:40 to clean the ITO conductive glass electrode, then rinse it with water and dry it in the air for use;

[0056] (2) The ITO conductive glass electrode after drying in step (1) is immersed in the mixed solution of zinc nitrate, potassium nitrate and manganese sulfate to carry out electrochemical deposition, is-0.8V relative to the deposition potential of silver / silver chloride electrode , the deposition temperature is 60°C, and the deposition time is 150min. After deposition, the Mn-doped dendritic zinc oxide nanorod photoanode is obtained, and the concentration of the zinc nitrate solution in the mixed solution is 0.8mmolL -1 , the concentration of potassium nitrate solution is 0.15molL -1 , th...

Embodiment 3

[0065] Embodiment 3: A construction method of a potassium ion nucleic acid aptamer photoelectrochemical sensor based on a dilute magnetic semiconductor, comprising the following steps:

[0066] (1) Use a mixed solution of ammonia, hydrogen peroxide and water with a volume ratio of 1:1:30 to clean the ITO conductive glass electrode, then rinse it with water and dry it in the air for use;

[0067] (2) The ITO conductive glass electrode after drying in the step (1) is immersed in the mixed solution of zinc nitrate, potassium nitrate and manganese sulfate to carry out electrochemical deposition, relative to the deposition potential of silver / silver chloride electrode is-1.2V , the deposition temperature is 100°C, and the deposition time is 90min. After the deposition, the Mn-doped dendritic zinc oxide nanorod photoanode is obtained, and the concentration of the zinc nitrate solution in the mixed solution is 0.3mmolL -1 , the concentration of potassium nitrate solution is 0.05molL ...

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Abstract

The present invention discloses a construction method and a detection method of a potassium ion nucleic acid aptamer photoelectrochemical sensor based on a diluted magnetic semiconductor. The construction method comprises: (1) washing a glass electrode; (2) preparing a Mn-doped dendritic zinc oxide nano-rod photoanode; (3) preparing a Mn-doped dendritic zinc oxide nano-rod photoanode film; (4) assembling a potassium ion nucleic acid aptamer on the Mn-doped dendritic zinc oxide nano-rod photoanode surface to obtain a potassium ion nucleic acid aptamer base photoelectrochemical sensor; and (5) fitting a quantitative relationship curve, and calculating the potassium ion content in a sample to be detected. According to the present invention, the Mn<2+>-doped dendritic zinc oxide diluted magnetic semiconductor nano-rod is electrochemically synthesized by using the one-step method so as to be adopted as the photoelectric beacon; and by fitting the quantitative relationship curve, the novel nucleic acid aptamer diluted magnetic semiconductor base photoelectrochemical sensor platform is constructed and is used for ultrasensitive detection of the potassium ions.

Description

technical field [0001] The invention belongs to the field of chemical analysis and testing, in particular to a construction and detection method of a dilute magnetic semiconductor-based potassium ion nucleic acid aptamer photoelectrochemical sensor. Background technique [0002] Potassium ion is an important cation in biological systems, which plays a variety of roles in biological processes, such as: nerve transmission, maintenance of muscle strength and extracellular permeability, enzyme activation, apoptosis, blood pressure Adjustment of pH value and control of other ion concentrations in living cells. Abnormal fluctuations in potassium ion concentrations are a hallmark of many diseases, including alcoholism, loss of appetite, bulimia, heart disease, diabetes, AIDS, cancer, and more. Therefore, the detection of potassium ions has important value and significance for the identification and diagnosis of diseases. Among various potassium ion detection methods, G-quadruplex...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 李静李红波张喜标潘志文
Owner YANCHENG INST OF TECH
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