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Test paper for detecting metalaxyl residue and application thereof

A metalaxyl and test paper technology, which is applied in the field of test paper for the detection of metalaxyl residues, can solve the problems of tedious and time-consuming test sample processing, and achieve the effects of short detection time, wide application range and simple operation

Inactive Publication Date: 2016-01-20
贵州勤邦食品安全科学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the perspective of existing technologies and related testing standards, the current detection methods for metalaxyl residues are mainly instrumental methods, and the most commonly used methods are LC-UV, LC-MS and LC-MS / MS. The instrumental methods have high detection sensitivity, Strong specificity and other advantages, but the pretreatment of the test sample is cumbersome and time-consuming, and the sample needs to be extracted and purified. At the same time, the instrument test method requires expensive large-scale instruments and equipment, and professional test technicians are equipped, so its application is limited; colloid Gold immunochromatography has the advantages of good specificity, high sensitivity, easy operation, low detection cost, and is suitable for screening and detection of batch samples. It is an ideal rapid screening method and can better meet the requirements of on-site rapid detection.

Method used

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  • Test paper for detecting metalaxyl residue and application thereof
  • Test paper for detecting metalaxyl residue and application thereof
  • Test paper for detecting metalaxyl residue and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 metalaxyl detection test paper

[0041] The preparation method of the test paper mainly includes the following steps:

[0042] 1) Prepare a conjugate release pad coated with a metalaxyl monoclonal antibody-colloidal gold marker;

[0043] 2) Prepare a reaction membrane with a detection line coated with a metalaxyl hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse antibody;

[0044] 3) Assemble the conjugate release pad, reaction membrane, sample absorption pad, water absorption pad and PVC bottom plate prepared in 1) and 2) into a test paper.

[0045] The following is a step-by-step detailed description:

[0046] 1. Preparation of metalaxyl hapten

[0047] Metalaxyl was dissolved in ethanol, 10% NaOH aqueous solution was added with stirring at room temperature, and the reaction was monitored by TLC until there was no raw material or the raw material point was very shallow. The reaction was stopped...

Embodiment 2

[0081] The detection of metalaxyl residue in the sample of embodiment 2

[0082] 1. Sample pretreatment

[0083] Take 1.0g±0.05g of homogeneous sample into a 50ml polystyrene centrifuge tube, add 5ml of extract, shake with a shaker for 5min, centrifuge at 3000g for 5min at room temperature, and take the supernatant for testing.

[0084] 2. Test with test paper

[0085] Use a pipette to suck up the sample solution to be tested and add 2-3 drops vertically to the sample addition hole. When the liquid flows, start timing and react for 5 minutes. The result is judged, and other times are judged invalid.

[0086] 3. Analysis of test results

[0087] Negative (-): Both the T line and the C line are colored, indicating that the metalaxyl concentration in the sample is below the detection limit, such as image 3 .

[0088] Positive (+): T line has no color and C line has color, indicating that the drug concentration of metalaxyl in the sample is equal to or higher than the detectio...

Embodiment 3

[0090] Example 3 Sample Detection

[0091] 1. Detection limit test

[0092] A blank tobacco sample was taken, and metalaxyl was added to it to a final concentration of 1, 2, and 4 mg / kg, respectively. Test paper was taken for detection, and the measurement was repeated three times for each sample.

[0093] When testing tobacco, tea, and vegetable samples with test paper, when the metalaxyl concentration is 1mg / kg, the test paper shows two red lines visible to the naked eye, which is negative; when the metalaxyl concentration is 2, 4mg / kg, the quality control line of the test paper is displayed, but the detection line is not displayed, which is positive; it indicates that the detection line of this test paper to metalaxyl is 2 mg / kg.

[0094] 2. False positive rate and false negative rate test

[0095] Take 20 positive tobacco samples with known metalaxyl content greater than 2 mg / kg and 20 negative tobacco samples with known metalaxyl content less than 2 mg / kg, and use thre...

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Abstract

The invention discloses a test paper for detecting metalaxyl residue and application thereof. The test paper comprises a sample absorption pad (1), a conjugate release pad (2), a reaction membrane (3), a water absorption pad (4) and a base plate (7), wherein the reaction membrane comprises a detection line (5) coated with a metalaxyl semiantigen-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antiantibody, and the conjugate release pad (2) is coated with a metalaxyl monoclonal antibody-colloidal gold marker. The invention also provides a method for applying the metalaxyl test paper in detection of metalaxyl residue in tea, tobacco and vegetable. The test paper provided by the invention can be used for detecting metalaxyl residue in tea, tobacco and vegetable, has the characteristics of simple operation, high sensitivity, a fast detection speed, low cost, etc., and is applicable to screening and on-site monitoring of a great number of samples.

Description

technical field [0001] The invention relates to a test paper for detecting metalaxyl residue and its application, in particular to a colloidal gold test paper for detecting metalaxyl residue, which is particularly suitable for qualitative detection of metalaxyl in tobacco. Background technique [0002] Metalaxyl, D,L-N-(2,6-dimethylphenyl)-N-(2'-methoxyacetyl)alanine methyl ester, is a novel protective and therapeutic effect It is a systemic fungicide that can be absorbed by the roots, stems and leaves of plants, and transferred to various organs of plants with the water movement in plants. It can be used for stem and leaf treatment, seed treatment and soil treatment. , The diseases caused by Pythium are effective, and are often used in agricultural production to treat grape downy mildew, cucumber downy mildew, cucumber angular spot, pepper blight, tobacco black shank, potato late blight, tomato late blight, rice standing blight Disease, rice bacterial wilt, rice bakanae di...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/577G01N33/558
Inventor 冯才伟谢体波陆苇何方洋汪善良易重任李平
Owner 贵州勤邦食品安全科学技术有限公司
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