Method and primer combination for quickly detecting trichinella spiralis on basis of LAMP (loop-mediated isothermal amplification) technologies

Abstract

The invention discloses a method and a primer combination for quickly detecting trichinella spiralis on the basis of LAMP (loop-mediated isothermal amplification) technologies. The LAMP primer combination for detecting the specificity of the trichinella spiralis comprises a primer F3, a primer B3, a primer FIP and a primer BIP. Sequences of the primers F3, B3, FIP and BIP are respectively shown as SEQ ID NO.1-SEQ ID NO.4. The primer combination can be applied to preparing reagents for detecting the trichinella spiralis. The method and the primer combination have the advantages that the primer combination and the corresponding method are high in specificity and sensitivity; different pathogens such as the trichinella spiralis, Escherichia coli, cysticerus cellulosae and toxoplasma gondii can be detected, and results can and only can be positively amplified from trichinella spiralis samples; the sensitivity of the method is 1000 times the sensitivity of common PCR (polymerase chain reaction) detection technologies, and the trichinella spiralis can be detected even if a gram of meat products only contains one muscle larva.

Description

technical field [0001] The invention belongs to the technical fields of veterinary biotechnology and molecular biology, and relates to a rapid detection method and a primer composition of Trichinella spiralis based on LAMP technology. Background technique [0002] Trichinellosis is a worldwide zoonotic parasitic disease, which is prevalent among mammals, and humans and animals are infected by eating raw or semi-cooked meat containing Trichinella cysts. The disease is very harmful, not only causing huge economic losses to animal husbandry production, but also posing a serious threat to human health. my country is one of the few countries where the disease is most harmful in the world, and it has been listed as the first of the three zoonotic parasitic diseases (trichinellosis, cysticercosis and echinococcosis), and as The import and export of meat, the slaughter of animals, and the zoonotic diseases that the Chinese government proposes to allow people to eat "safe meat" are t...

AI Technical Summary

Problems solved by technology

At present, microscopic examination and digestion are mainly used in the quarantine of animal trichinellosis in my country, which has the advantages of simple operation, etc., but requires the quarantine personnel to have good professional knowledge and experience, and the labor intensity of the staff is high. In addition, meat infection It is easy to miss detection when the intensity is low

PCR diagnostic technology has greatly improved the sensitivity of inspection and quarantine methods. However, due to unfavorable factors such as the high price of PCR instruments and the need for electrophoresis analysis of amplified products, the application of this method is greatly restricted.

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