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Extraction method for laminarin

An extraction method and technology of kelp polysaccharides, which are applied in the field of polysaccharide preparation, can solve the problems of reduced biological activity of polysaccharides, large amount of enzymes used in enzyme extraction, and low extraction efficiency, and achieve strong antimicrobial ability, short production cycle, and improved The effect of extraction rate

Inactive Publication Date: 2016-02-03
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the disadvantages of low extraction efficiency and time-consuming of seaweed polysaccharide water extraction method in the prior art, acid extraction method is likely to cause polysaccharide hydrolysis, alkali extraction method is likely to cause polysaccharide biological activity to decrease, enzyme extraction method has a large amount of enzyme and high cost, the present invention provides An economical, energy-saving, non-environmental pollution, safe and non-toxic, high extraction rate, high purity, high titer of biologically active components, and an extraction method for laminarin that can be industrialized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting laminarin, which undergoes the following process steps:

[0026] (1) Preparation of raw materials

[0027] Select fresh kelp as a raw material, wash and dry it, crush it, and pass through a 100-mesh sieve to obtain kelp powder for subsequent use;

[0028] (2), degreasing

[0029] Add the kelp powder obtained in step (1) to 8 times its weight in 95% ethanol and soak for 18 hours, reflux and extract for 2 hours, repeat 2 times, remove ethanol, and dry the solid to obtain degreased kelp powder;

[0030] (3), extraction

[0031] Add 10 times the weight of pure water to the degreased kelp powder obtained in step (2), control the temperature at 80°C, extract for 6 hours, and extract 3 times; combine the extracts, centrifuge, prepare the supernatant, and set aside;

[0032] (4), concentrated

[0033] The supernatant prepared in step (3) is heated until the volume is reduced to less than 3 times of the kelp feeding amount to obtain a concentrated sol...

Embodiment 2

[0039] A method for extracting laminarin, which undergoes the following process steps:

[0040] (1) Preparation of raw materials

[0041] Select fresh kelp as a raw material, wash and dry it, crush it, and pass through a 200-mesh sieve to obtain kelp powder for subsequent use;

[0042] (2), degreasing

[0043] The kelp powder prepared in step (1) was added to 10 times its weight in 95% ethanol, soaked for 12 hours, refluxed for 3 hours, repeated once, the ethanol was removed, and the solid was dried to obtain degreased kelp powder;

[0044] (3), extraction

[0045] Add 20 times the weight of pure water to the degreased kelp powder obtained in step (2), control the temperature at 60° C., extract for 8 hours, and extract twice; combine the extracts, centrifuge, prepare the supernatant, and set aside;

[0046] (4), concentrated

[0047] The supernatant prepared in step (3) is heated until the volume is reduced to less than 5 times of the kelp feeding amount to obtain a concen...

Embodiment 3

[0053] A method for extracting laminarin, which undergoes the following process steps:

[0054] (1) Preparation of raw materials

[0055] Select fresh kelp as a raw material, wash and dry it, crush it, and sieve it to obtain kelp powder for later use;

[0056] (2), degreasing

[0057] The kelp powder obtained in step (1) is added to 95% ethanol which is 4 times its weight, soaked for 24 hours, refluxed for 1 hour, repeated 3 times, the ethanol is removed, and the solid is dried to obtain degreased kelp powder;

[0058] (3), extraction

[0059] Add 4 times the weight of pure water to the degreased kelp powder obtained in step (2), control the temperature at 100°C, extract for 2 hours, and extract 5 times; combine the extracts, centrifuge, prepare the supernatant, and set aside;

[0060] (4), concentrated

[0061] The supernatant liquid prepared in step (3) is heated until the volume is reduced to 4 times of the sea-tangle charging amount, and the concentrated solution is obta...

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Abstract

The invention discloses an extraction method for laminarin. The method includes the steps of airing and smashing fresh laminaria japonica to obtain laminaria japonica powder, conducting hot water extraction after conducting ethyl alcohol degreasing on the laminaria japonica powder, centrifuging the extracting solution to obtain supernate, conducting concentration and impurity removal on the supernate, conducting microfiltration film filtration, sequentially classifying filtrate, conducting ultrafiltration through filter materials of different molecular weights, and conducting drying to obtain laminarin of different molecule classifications. No high-temperature or chemical changes are caused in the operation process, it is sufficiently ensured that activity of polysaccharide and protein is not damaged, and components are highly enriched; by means of the combined effects of centrifugation, microfiltration and ultrafiltration, the high flow rate is kept, the obtained polysaccharide is high in purity and diverse in specification, and the extraction method has the advantages that continuous production and automatic operation are achieved, the energy consumption is low and the production period is short.

Description

technical field [0001] The invention relates to the preparation of polysaccharides, in particular to a method for extracting laminarin. Background technique [0002] Kelp ((Laminaria JaponicaAresch), also known as Lunbu, Chinese cabbage, medicinally known as kelp, is a large seaweed of the genus Laminaria Laminaria Laminaria of Phaeophyta. Laminaria is rich in a variety of functional ingredients and has extremely high edible and medicinal value It is recorded in the ancient books "Compendium of Materia Medica", "Shen Nong's Materia Medica", "Diet Therapy Materia Medica", etc., especially in recent years, it has been found that kelp has the functions of immune regulation, anti-tumor, anti-coagulation, lipid-lowering, hypoglycemic, It has remarkable effects in anti-oxidation and other aspects, so it is often called "longevity food" and "health food", and is deeply loved by people. [0003] Studies have shown that most of the biological activities of kelp are closely related t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 齐君刘昌衡孙永军张绵松胡炜袁文鹏鞠文明唐晓波
Owner SHANDONG HOMEY AQUATIC DEV
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