Method of fermenting Bt by taking blue-green algae biogas slurry as matrix
A technology of cyanobacteria marsh and matrix, applied in the field of pesticides, can solve the problems of inconvenient use, perishable fermentation of cyanobacteria, etc., and achieve good dispersion, improved reuse rate, and good quality
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[0022] The present invention will be described in detail through the following examples. The instrument used in this example is a conventional commercially available instrument.
[0023] The method of the present invention for fermenting Bt with cyanobacteria biogas slurry as a substrate has the following specific steps:
[0024] (1) Raw material selection:
[0025] The composition and mass percentage are: 93.12% of Microcystis, 1.66% of Anabaena, 1.72% of Flospora, 2.99% of Chlorophyta, and 0.51% of fresh cyanobacteria of Diatom;
[0026] Strain standard number: FSCC(T)115030, GIMCC number: GIM1.32 Bacillus thuringiensis; the strain was purchased from Guangdong Microbial Strain Collection on June 18, 2013, address: 100 Xianlie Middle Road, Guangzhou The fifth floor of No. Laboratory Building.
[0027] Conventional LB liquid medium: tryptone: 40%, yeast extract: 20%, sodium chloride 40%; or conventional cyanobacteria medium with solid content of 1-3%.
[0028] (2) Preparation of cyanob...
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