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Method for separating and purifying Zhisuanpaixi in paramecium

A technology for the separation and purification of phytopine, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of difficult separation and purification, increased processing costs, and high product impurities, and achieve simple extraction methods , high production efficiency and low cost

Inactive Publication Date: 2016-02-17
仇颖超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention: in view of the difficulty in separation and purification in the process of extracting phytene in the current method, a large amount of solvent needs to be added for separation and purification, resulting in the disadvantages of high impurities, low purity and increased processing cost of this product, and a method is provided. The method for separating and purifying pinene phytate from planting paramecium, in the present invention, the crude pinene phytate is firstly extracted with the assistance of ultrasound to prepare a paramecium culture solution, and after the paramecium adapts to and absorbs the pinene phytide culture solution to reproduce in large quantities, it is then separated Purified phytic acid pinene

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] Firstly, during the paddy field irrigation period, look for the old rice stubble in the field, put the beaker in a 95°C oven for 2 hours, and then use the sterilized beaker to take 500mL of water containing paramecium at 20cm around the rice stubble, and select fresh rice straw At 2cm above the root, cut a 3cm-long section of straw and place it in a beaker. After the collection is completed, place the beaker at 25°C with a light intensity of 120μmolm -2 the s -1 , cultivated it for 6 days, and set it aside; then put the saw palmetto fruit in the sun for 3 days, make it dehydrated and dried, select the dehydrated and dried saw palmetto fruit, put it in a jet mill and grind it Sieve it to prepare 60-mesh saw palmetto fruit powder, stir and mix ethyl acetate and saw palmetto fruit powder at a mass ratio of 10:1, heat it to 60°C after it is mixed evenly, and Under the speed of 1200r / min, ultrasonic-assisted extraction at 200W was carried out for 1 hour; after the ultrasoni...

example 2

[0018]First, during the paddy field irrigation period, look for the old rice stubble in the field, put the beaker in a 97°C oven for 2.5 hours, and then use the sterilized beaker to take 750mL of water containing paramecium at 25cm around the rice stubble, and select fresh At 2.5cm above the root of the straw, cut a 4cm-long section of straw and put it in a beaker. -2 the s -1 , cultivate it for 7 days, and set it aside; then place the saw palmetto fruit in the sun for 4 days, make it dehydrated and dry, select the dehydrated and sun-dried saw palmetto fruit, put it in a jet mill and grind it. Sieve it to prepare 70-mesh saw palmetto fruit powder, stir and mix ethyl acetate and saw palmetto fruit powder at a mass ratio of 10:1, and heat it to 65°C after it is evenly mixed. Under the speed of 1350r / min, ultrasonically assisted extraction at 250W for 1.5h; after the ultrasonically assisted extraction was completed, centrifuged in a centrifuge at 2500r / min for 12min, the upper e...

example 3

[0021] First, during the paddy field irrigation period, look for the old rice stubble in the field, put the beaker in a 100°C oven for 3 hours to sterilize, then use the sterilized beaker to take 1000mL of water containing paramecium at a distance of 30cm around the rice stubble, and select fresh rice straw At 3cm above the root, cut off a 5cm-long straw section and place it in a beaker. After the collection is completed, place the beaker at 30°C with a light intensity of 140μmolm -2 the s -1 , cultivate it for 7 days, and set it aside; then place the saw palmetto fruit in the sun for 5 days, make it dehydrated and dry, select the dehydrated and sun-dried saw palmetto fruit, put it in a jet mill and grind it Sieve it to prepare 80-mesh saw palmetto fruit powder, stir and mix ethyl acetate and saw palmetto fruit powder at a mass ratio of 10:1, and heat it to 70°C after it is evenly mixed. Under the speed of 1500r / min, ultrasonic-assisted extraction at 300W was carried out for ...

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Abstract

The invention discloses a method for separating and purifying Zhisuanpaixi (which is specially contained in serenoa repens (Bartram) Small extractive and is capable of directly destroying pathogene DNA polymerase, destroying drug-resistant strain and killing germs plasmid) in paramecium, and belongs to the field of Zhisuanpaixi extraction technology. Zhisuanpaixi is obtained according to the following steps: firstly getting an aqueous solution of paramecium, culturing paramecium for usage, then insolating serenoa repens (Bartram) Small, crushing to form a powder, and then performing ultrasonic-assisted extraction to obtain a Zhisuanpaixi crude product, mixing with a tawny straw mixed solution prepared from straw, so as to obtain a paramecium culture solution, and after paramecium is adaptive and absorbs the Zhisuanpaixi culture solution and is subjected to mass propagation, performing separation and purification. The beneficial effects comprise that Zhisuanpaixi extracted through the method is low in impurity and high in purity, the purity reaches 99.2% or more, and the method does not need to add any solvents, and is green, safe and low in cost. The extraction method is simple, the obtained product is easy to separate and purify, and the production efficiency is high.

Description

technical field [0001] The invention relates to a method for separating and purifying pinene phytate from paramecium, and belongs to the technical field of pinene phytate extraction. Background technique [0002] Saw palmetto, also known as saw palmetto leaves or saw palmetto, scientifically named "Sabah palm", belongs to the shrubs of the palm family. The ripe, dried fruit of the leaf palm is used medicinally. The extract of Saw Palmetto fruit is a powerful natural treatment for BPH. Saw palmetto extract can effectively inhibit the activity of 5a-reductase, reduce the production of dihydrotestosterone, antagonize the combination of androgen and androgen receptor in prostate tissue; it has adrenergic antagonism and calcium blocking effect, so as to improve bladder function, Antispasmodic effect; it can also inhibit the activity of cyclooxygenase and lipoxygenase, reduce the production of leukotrienes, prostaglandins and other inflammatory mediators, thus play an anti-infla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C13/40C07C13/42C07C7/00C12N1/10C12R1/90
CPCC07C7/005C12N1/10C07C13/40C07C13/42
Inventor 仇颖超高力群盛艳花
Owner 仇颖超
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