Method for extracting and isolating gallinylic acid from Qiongdao dyed wood tree

Abstract

The invention discloses a method for extracting and separating paederosidic acid from Saprosma merrillii Lo. The method comprises the following concrete steps: (1) extracting the stem of Saprosma merrillii Lo by using an aqueous ethanol solution and concentrating liquid extract so as to obtain ethanol extract; (2) dispersing the ethanol extract in water, successively extracting the dispersed ethanol extract with petroleum ether and ethyl acetate of a same volume, dispersing a residual water phase part left after extraction in water, carrying out macroporous resin chromatography, then carrying out gradient elution with the aqueous ethanol solution, carrying out silica gel column chromatography on a target fraction and then carrying out gradient elution with chloroform-methanol so as to obtain a crude paederosidic acid product with a purity of no less than 50%; and (3) dissolving the crude paederosidic acid product with methanol and then carrying out gradient elution with chloroform-methanol so as to obtain a fine paederosidic acid product with a purity of no less than 90%. The method provided by the invention pioneers extraction of the active ingredient paederosidic acid from Saprosma merrillii Lo and has active significance to comprehensive utilization of the medicinal material resource of Saprosma merrillii Lo.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective plant components, and in particular relates to a method for extracting and separating gallinylic acid from Qiongdao dyed wood trees. Background technique [0002] At present, it has been reported in the literature to extract and separate thioiridoid glycosides, such as gallinacein, gallininic acid, and gallininic acid methyl ester, in which gallinacein has been It has been proven to have good anti-inflammatory and analgesic effects, and can be used to prepare analgesic and / or anti-inflammatory drugs (see Chinese patent document CN101890033A). The structural formula of gallic acid is as follows: [0003] . [0004] Qiongdao stained wood tree (S.merrillii Lo) is a plant of the Rubiaceae (Rubiaceae) genus Saprosma. It is a unique plant species in Hainan Island. It is distributed in Bawangling and other places in Hainan Province. So far, no dyed wood from Qiongdao has...

AI Technical Summary

Problems solved by technology

[0004] Qiongdao stained wood tree (S.merrillii Lo) is a plant of the Rubiaceae (Rubiaceae) genus Saprosma. It is a unique plant species in Hainan Island. It is distributed in Bawangling and other places in Hainan Province. So far, no dyed wood from Qiongdao has been found. Bibliographical reports on the extraction and isolation of gallinylic acid from woody trees