Method for extracting and isolating gallinylic acid from Qiongdao dyed wood tree

A technology for extracting gallic acid and ethanol, which is applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of extraction and separation of gallic acid that have not been found, and achieve strong operability and process Systematic and highly reproducible results

Inactive Publication Date: 2019-05-14
HAINAN NORMAL UNIV
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Problems solved by technology

[0004] Qiongdao stained wood tree (S.merrillii Lo) is a plant of the Rubiaceae (Rubiaceae) genus Saprosma. It is a unique plant species in Hainan Island. It is distributed in Bawangling and other places in Hainan Province. So far, no dyed wood from Qiongdao has been found. Bibliographical reports on the extraction and isolation of gallinylic acid from woody trees

Method used

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Embodiment 1）

[0017] The method for extracting and isolating gallinylic acid from Qiongdao stained wood tree of the present embodiment has the following steps:

[0018] ① Extract the dried and pulverized Qiongdao dyed tree stem powder (10kg) with a volume concentration of 75% ethanol solution (10kg) three times (5 days for the first time and 7 days for the second time) after drying and crushing. , the third 8 days), the combined extracts were concentrated under reduced pressure to obtain a brown viscous ethanol extract (437g).

[0019] ② Disperse the ethanol extract obtained in step ① evenly in 1000mL distilled water, extract three times with equal volumes of petroleum ether (1000mL) and ethyl acetate (1000mL), combine the extracts, and concentrate under reduced pressure to obtain the organic phase (petroleum ether part 30g and ethyl acetate part 20g), the remaining water phase part (240g) was redispersed in 500mL distilled water, stirred evenly and then subjected to macroporous resin chrom...

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Abstract

The invention discloses a method for extracting and separating paederosidic acid from Saprosma merrillii Lo. The method comprises the following concrete steps: (1) extracting the stem of Saprosma merrillii Lo by using an aqueous ethanol solution and concentrating liquid extract so as to obtain ethanol extract; (2) dispersing the ethanol extract in water, successively extracting the dispersed ethanol extract with petroleum ether and ethyl acetate of a same volume, dispersing a residual water phase part left after extraction in water, carrying out macroporous resin chromatography, then carrying out gradient elution with the aqueous ethanol solution, carrying out silica gel column chromatography on a target fraction and then carrying out gradient elution with chloroform-methanol so as to obtain a crude paederosidic acid product with a purity of no less than 50%; and (3) dissolving the crude paederosidic acid product with methanol and then carrying out gradient elution with chloroform-methanol so as to obtain a fine paederosidic acid product with a purity of no less than 90%. The method provided by the invention pioneers extraction of the active ingredient paederosidic acid from Saprosma merrillii Lo and has active significance to comprehensive utilization of the medicinal material resource of Saprosma merrillii Lo.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective plant components, and in particular relates to a method for extracting and separating gallinylic acid from Qiongdao dyed wood trees. Background technique [0002] At present, it has been reported in the literature to extract and separate thioiridoid glycosides, such as gallinacein, gallininic acid, and gallininic acid methyl ester, in which gallinacein has been It has been proven to have good anti-inflammatory and analgesic effects, and can be used to prepare analgesic and / or anti-inflammatory drugs (see Chinese patent document CN101890033A). The structural formula of gallic acid is as follows: [0003] . [0004] Qiongdao stained wood tree (S.merrillii Lo) is a plant of the Rubiaceae (Rubiaceae) genus Saprosma. It is a unique plant species in Hainan Island. It is distributed in Bawangling and other places in Hainan Province. So far, no dyed wood from Qiongdao has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/04C07H1/08
CPCC07H1/08C07H17/04
Inventor 陈光英陈文豪韩长日宋小平张俊燕
Owner HAINAN NORMAL UNIV
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