Novel adjuvant vaccine composition associated with HPV

A vaccine composition and a technology for the composition, applied in the field of vaccine compositions, can solve the problems of aluminum adjuvant safety concerns, adverse reactions in the nervous system, weak immune effect of the adjuvant, etc., and achieve protection against virus infection, simple preparation method, and inflammation. light effect

Active Publication Date: 2016-03-02
张世艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, aluminum adjuvants can cause IgE-mediated allergic reactions (such as injection site granulomas) and adverse reactions to the nervous system, thereby causing people to worry about the safety of aluminum adjuvants (Petrik, Wong et al.2007, Bystrianyk2009, ShawandPetrik2009, Munks , McKee et al. 2010, Tomljenovic and Shaw 2011)
In addition, the adjuvant immune effect induced by aluminum adjuvant on influenza (Atmar and Keitel2009), malaria (Lew, Anders et al.1988, Schwartz, Brownetal.2012), herpes simplex virus (Geerligs, Weijere et al.1989) is relatively weak

Method used

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  • Novel adjuvant vaccine composition associated with HPV
  • Novel adjuvant vaccine composition associated with HPV
  • Novel adjuvant vaccine composition associated with HPV

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Preparation of composite particles of calcium phosphate and polymer PLGA

[0046] The PLGA of 100mg is dissolved in the dichloromethane of 20mL as oil phase (O), the 0.1M calcium chloride solution of preparation 0.2mL is as inner water phase (W1), the PVA solution of preparation 1.0% is as outer water phase (W2 ); 0.2mL of the inner water phase (W1) was added to the oil phase (O), and the colostrum W1 / O was prepared by homogeneous emulsification method, and the first emulsion was added to 60mL of the outer water phase to prepare the pre-multiplex emulsion (W1 / O / W2), this pre-multiplex emulsion is repeatedly pressed through the membrane by the method of rapid membrane emulsification to prepare a water-in-oil-in-water complex emulsion (W1 / O / W2) with uniform particle size; then add to this complex emulsion 0.1mL of 0.05M disodium hydrogen phosphate solution, using the solute diffusion of the inner and outer water phases during the solidification process to m...

Embodiment 2

[0047] Embodiment 2: Preparation of composite particles of calcium phosphate and polymer PLGA

[0048] Dissolve 100mg of PLGA in 20mL of dichloromethane as the oil phase (O), prepare a 1.0% PVA solution as the external water phase (W2), add the oil phase (O) to 50mL of the external water phase (W2) , prepare the pre-emulsion (W2 / O) of oil-in-water type, this pre-multiple emulsion adopts the method for rapid film emulsification to repeatedly press through the membrane to prepare the water-in-oil-in-water double emulsion (W1 / O / O) with uniform particle size W2); solidified for 3h, centrifuged and washed, freeze-dried to obtain dry PLGA microspheres, and the SEM photo of PLGA is as follows figure 2 shown. Then suspend the prepared PLGA microspheres in 10mL ultrapure water to obtain PLGA microsphere suspension, prepare 0.2mL of 0.2M calcium chloride solution and 0.1M disodium hydrogen phosphate solution, and first mix 0.2mL of 0.2M Calcium chloride solution was added to 10mL of ...

Embodiment 3

[0049] Embodiment 3: the preparation of the composite particle of calcium phosphate and polymer PLGA

[0050] The PLGA of 100mg is dissolved in the dichloromethane of 20mL as oil phase (O), the 0.6M calcium chloride solution of preparation 0.2mL is as inner water phase (W1), the PVA solution of preparation 1.0% is as outer water phase (W2 ); 0.2mL of the inner water phase (W1) was added to the oil phase (O), and the colostrum W1 / O was prepared by homogeneous emulsification method, and the first emulsion was added to 60mL of the outer water phase to prepare the pre-multiplex emulsion (W1 / O / W2), this pre-multiplex emulsion is repeatedly pressed through the membrane by the method of rapid membrane emulsification to prepare a water-in-oil-in-water complex emulsion (W1 / O / W2) with uniform particle size; then add to this complex emulsion 0.1mL of 0.3M disodium hydrogen phosphate solution, using the solute diffusion of the inner and outer water phases during the solidification proces...

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Abstract

The present invention discloses a novel adjuvant vaccine associated with HPV, and a preparation method thereof, wherein specifically the novel adjuvant is PLGA-CaP composite particles, and the adjuvant vaccine PLGA-CaP composite particles can produce in vivo immune response, and belongs to the developed efficient HPV protein vaccine composition.

Description

technical field [0001] The invention relates to a vaccine composition using calcium phosphate-polymer composite particles as an adjuvant, a preparation method of the vaccine composition and its application in medicine. The present invention is especially aimed at the vaccine composition made of HPV protein and its application in medicine for treating or preventing HPV virus. Background technique [0002] Human papillomaviruses (HPV) are non-enveloped double-stranded DNA viruses, mainly composed of viral shell and genomic DNA (King, Adams et al. 2012). The HPV viral coat is an icosahedral structure composed of 360 L1 proteins (forming 72 pentamers) and up to 72 L2 proteins, with a diameter of 55-60 nm (Howley and Lowy 2007). Viral coat proteins have self-assembly properties. In vitro, L1 protein self-assembles alone or together with L2 protein to form virus-like particles (Virus-likeParticle, VLP) (Chen, Garcea et al. 2000, Clements and Griffiths 2002, Chen, Nietal. 2011, Wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K47/34A61K47/04A61P31/20A61K39/12
Inventor 杨小杰张世艳
Owner 张世艳
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