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Brain protein hydrolysate preparation method

A technology of cerebroprotein hydrolyzate and enzymatic hydrolysis, which is applied in the field of biochemical pharmacy, can solve the problems of high hygroscopicity, which is not conducive to the production of cerebroprotein hydrolyzate tablets, high viscosity of cerebroprotein extract powder, and large amount of organic solvent. Improve the function of brain cells, reduce the degree of emulsification and viscosity, and restore brain vitality

Inactive Publication Date: 2016-03-16
卫材(辽宁)制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production efficiency is low, the extract contains a lot of fat, the nanofiltration production is cumbersome, the cerebroprotein extract powder has high viscosity and high hygroscopicity, which is not conducive to the production of cerebroprotein hydrolyzate tablets, etc.
At the same time, the obtained dry powder has high viscosity and low yield
There is also directly degreasing with acetone after the homogenate, but the organic solvent consumption is large and the effect is not as good as the method of the present invention

Method used

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  • Brain protein hydrolysate preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. Raw material pretreatment dehydration and degreasing:

[0023] Take the pig brain, melt it at room temperature to remove the fascia and mince it, then dry it in a vacuum drying oven at 50°C and a vacuum degree below -0.085MPa, and grab a plate immediately after drying (strictly control the drying temperature not to exceed 60°C) to obtain brain dry powder .

[0024] Take the dry brain powder and soak it in acetone with 3 times the mass of the dry powder for 18 hours, then change the acetone to soak, repeat the treatment 3 times, then filter, press the filter residue, and vacuum dry the filter residue at 50 °C for 12 hours; Stir every hour.

[0025] 2. Trypsin hydrolysis:

[0026] Add the dried filter residue to 10 times (w / w) water of the filter residue, stir evenly, adjust the pH of the system with ammonia to 8.0, heat it in a water bath to 52°C, add 4% (w / w) of the above-mentioned dried filter residue / W) trypsinogen powder, under 52 DEG C, keep stirring and hydr...

Embodiment 2

[0032] 1. Raw material pretreatment dehydration and degreasing:

[0033] Take the pig brain, melt it at room temperature to remove the fascia, shred it, and dry it in a vacuum drying oven at 60°C with a vacuum degree below -0.085MPa. After drying, grab a plate immediately (strictly control the drying temperature not to exceed 60°C) to obtain brain dry powder .

[0034] Take the dry brain powder and soak it in acetone with 2 times the mass of the dry powder for 20 hours, then change the acetone to soak, repeat the treatment twice, then filter, press the filter residue, and vacuum dry the filter residue at 50°C for 12 hours; Stir every hour.

[0035] 2. Trypsin hydrolysis:

[0036] Add the dried filter residue to 12 times (w / w) water of the filter residue, stir evenly, use ammonia to adjust the pH of the system to 8.5, heat it in a water bath to 48°C, and add 5% (W / w) of the above-mentioned dried filter residue W) trypsinogen powder, heat-preserved and stirred at 48°C for 4 h...

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Abstract

The present invention belongs to the technical field of chemical industry pharmacy, and specifically relates to a brain protein hydrolysate preparation method, which comprises: soaking pre-treated and dried pig brain 2-3 times with acetone, defatting, and drying; and hydrolyzing with trypsin, separating the enzyme hydrolyzing mixing liquid after the hydrolyzing so as to collect the supernatant, carrying out purification concentration on the supernatant, and drying to obtain the brain protein hydrolysate. According to the present invention, the extract has characteristics of brain cell nourishing and brain capillary dredging, achieves functions of brain activity restoring, brain cell function improving and memory increasing, and can completely improve hypomnesis and other brain atrophy symptoms; and the organic solvent consumption during the defatting process of the method of the present invention is reduced, the prepared brain protein hydrolysate is the powder, has low viscosity, and is suitable for the subsequent production of the brain protein hydrolysate tablet preparation, and the content is higher than the existing technical standard.

Description

technical field [0001] The invention belongs to the technical field of biochemical pharmacy, in particular to a preparation method of cerebroprotein hydrolyzate. Background technique [0002] The current extraction method is to take the pig brain tissue, remove the fascia, and make a brain homogenate. Then degreasing, enzymatic hydrolysis, ultrafiltration and nanofiltration and other processes for extraction. [0003] The specific enzymolysis process is: take pepsin, put in the hydrolysis (extraction) tank that has added purified water, and stir. Take pig brain homogenate, add it to the above pepsin aqueous solution, use 10% HCl solution to adjust the pH of the solution to 1.8-2.0, keep the temperature of the material at 41°C±1°C, continuously adjust the pH to 1.8-2.0, and hydrolyze for 4-6 hours . Adjust the pH value of the pepsin hydrolyzate to 7.0-8.0 with 10% NAOH solution, add trypsin, keep the temperature at 41°C±1°C, continuously adjust the solution to 7.0-8.0, and...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61P25/28
Inventor 贾天亮郑桂荣刘绍杰
Owner 卫材(辽宁)制药有限公司
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