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Ferulic acid esterase and its application

A ferulic acid esterase and amino acid technology, applied in the field of genetic engineering, can solve problems such as limited application, achieve high hydrolysis efficiency and promote hydrolysis

Active Publication Date: 2018-11-30
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic activity and enzymatic characteristics of ferulic acid esterase still limit its application

Method used

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  • Ferulic acid esterase and its application
  • Ferulic acid esterase and its application
  • Ferulic acid esterase and its application

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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0029] It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

[0030] The present invention provides a ferulic acid esterase, characterized in that the amino acid sequence of the ferulic acid esterase is the amino acid sequence shown in SEQ ID NO.1.

[0031] In a preferred version, the gene sequence for encoding the amino acid sequence of the ferulic acid esterase is shown in (a) or (b):

[0032] (a) the polynucleotide sequence shown in SEQ ID No.2;

[0033] (b) A polynucleotide sequence complementary to the polynucleotide sequence in (a) according to the principle of complementary base pairing.

[0034] Application of a ferulic acid ester...

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Abstract

The invention relates to the technical field of genetic engineering, in particular to a feruloyl esterase. The feruloyl esterase is found for the first time during genomic sequencing of penicillium piceum, and the sequence of the feruloyl esterase is quite new and is similar to the amino acid sequence of feruloyl esterase of common aspergillus flavus and aspergillus oryzae by 60% only. The invention further discloses a method for lignocellulose degradation by means of the feruloyl esterase. According to the method, the feruloyl esterase and cellulose are mixed and then added to a lignocellulose solution subjected to hydrothermal pretreatment for lignin degradation, the additive amount of the feruloyl esterase is 20-120 micrograms for each gram of substrate, and the additive amount of the cellulase is 10 FPU for each gram of substrate. The feruloyl esterase can promote hydrolysis of lignocellulose materials subjected to pretreatment in different ways after being blended with commercial cellulose, and hydrolysis efficiency is obviously higher than that of other types of feruloyl esterase within the same time period under the same hydrolysis condition.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a ferulic acid esterase and its application. Background technique [0002] Ferulic acid esterase (E.C.3.1.1.73, Ferulicacid esterases, FAE) is also called cinnamic acid esterase. It is a kind of carboxylesterase, which refers to the enzyme that can hydrolyze the ester bonds in ferulic acid methyl ester, oligosaccharide ferulic acid ester and polysaccharide ferulic acid ester to release ferulic acid. Ferulic acid plays an important role in the structure of plant cell walls. It can form junctions between lignin and lignin, between lignin and hemicellulose, and between hemicellulose and hemicellulose in plant cell walls, thereby Form a skeleton structure, making the entire cell wall hard. In biomass materials, ferulic acid is mainly connected to the arabinose residues of arabinoxylan in the form of ester bonds, which constitutes the link between lignin and hemicellulos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/18C12N15/55C12P19/14C12P19/02
CPCC12N9/18C12P19/02C12P19/14C12Y301/01073
Inventor 高乐王敏张东远陈树林
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI