Immobilized composite hair removing enzyme adopting porous carboxymethyl cellulose-polyvinyl alcohol microspheres as carrier, and preparation method thereof

Abstract

The present invention relates to the technical field of tanning chemistry and engineering, and provides an immobilized composite hair removing enzyme adopting porous carboxymethyl cellulose-polyvinyl alcohol microspheres as a carrier, and a preparation method thereof. According to the present invention, the immobilized composite hair removing enzyme adopts porous carboxymethyl cellulose-polyvinyl alcohol microspheres as an enzyme immobilizing carrier, the carrier has characteristics of good hydrophilicity, high mechanical strength and more pores, the microspheres provide good physical adsorption property for enzyme due to the porosity so as to effectively achieve the enzyme immobilization and improve the solid loading amount and the activity of the enzyme, and the carrier further has characteristics of good stability and reusability; and with the application of the immobilized composite hair removing enzyme in raw skin hair removing and collagen fiber dispersion, the production conditions are mild, the controllability on the skin collagen hydrolysis process and the collagen fiber separating loosening degree is improved, and the fluctuation of the product quality during the production can be avoided.

Description

Technical field [0001] The invention relates to the technical field of preparation of an immobilized enzyme special for tanning, in particular to an immobilized composite depilatory enzyme using porous carboxymethyl cellulose-polyvinyl alcohol microspheres as a carrier and a preparation method thereof. Background technique [0002] Hair removal is the process of removing or dissolving hair from the dermis through a series of physical and chemical methods. The traditional gray-alkali depilation method has good depilation effect, clean and rapid, simple operation, easy to control, but it has a good effect on loosening collagen fibers, and it does not damage the skin much, and the grain of the naked skin is clear. However, the pollution of sulfide is serious, and the hydrolysis of a large amount of keratin makes the content of organic matter in the wastewater greatly increased, and a large amount of alkaline sludge is produced, which is difficult to treat. Compared with the traditi...

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Problems solved by technology

[0003] However, the enzymatic hair removal method currently used in the leather industry mainly has the following disadvantages: (1) The stability of the enzyme preparation is poor and it is easy to be inactivated; These materials are easy to cause the inactivation of the enzyme; (3) the catalytic activity of the enzyme is too high, and the process controllability is poor

Therefore, it is necessary to study the application of immobilized enzymes in the field of tanning to form the effect of protection and slow release, enhance the durability of special enzyme preparations for tanning and prolong the enzymatic reaction time, but in the technology of immobilized enzymes, the carrier The selection of immobilized enzymes plays a vital role in the activity and use characteristics of immobilized enzymes. In order to obtain immobilized enzymes with good catalytic performance, high loading capacity and low cost, the existing technology uses low-cost, easy-to-obtain natural polymers , such as: activated carbon, diatomaceous earth, alum, chitosan, and calcium alginate as immobilization carriers, among which chitosan and calcium alginate have been extensively studied as immobilization carriers, but these natural polymers are used as immobilization carriers. When immobilized enzyme carriers are used, due to their limited compatibility and affinity with the enzyme, the immobilized enzyme in the obtained immobilized enzyme has low immobilization capacity and activity

Moreover, in conventional enzymatic hair removal, most of the hair removal enzymes have no specific effect on the intradermal components. They can hydrolyze a large amount of collagen while removing hair. cannot be completely removed

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