Detection method for residual quantity of sulfur dioxide in food and Chinese herbal medicines

A sulfur dioxide and detection method technology, which is applied in the preparation of test samples, measurement devices, color/spectral characteristics measurement, etc., can solve the problems of long pre-processing time, method system error, poor reproducibility, etc., and reduce interference , Improve the accuracy and improve the detection efficiency

Inactive Publication Date: 2016-03-16
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the detection of sulfur dioxide residues in food and Chinese herbal medicines has defects such as too long pretreatment time, difficult control of method system errors, and poor reproducibility.

Method used

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  • Detection method for residual quantity of sulfur dioxide in food and Chinese herbal medicines

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Embodiment 1

[0042] The present invention is applied to the detection of residual sulfur dioxide in Chinese herbal medicine, comprising the following steps:

[0043] (1) Draw a standard curve. With 490nm as the excitation wavelength, the concentration of the sulfite standard substance is determined to be 0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.1, and 0.2mM. The fluorescence intensity F of the standard solution at an emission wavelength of 520nm; Fluorescence intensity F at 520nm 0 , ΔF=F-F 0， Take the sulfite concentration C as the X axis and ΔF as the Y axis to draw a standard curve, see figure 1 ;

[0044] (2) the Chinese herbal medicine honeysuckle of the sample to be tested is pulverized with a grinder, and then accurately weighed 20.00g;

[0045] (3) Put the sample into the distillation bottle of the automatic distillation device, add 100mL of deionized water, place the absorption tube in 5mL of 5g / L sodium hydroxide absorption solution, and add 10mL of 1+1 hydrochloric acid ( Hydroch...

Embodiment 2

[0049] The present invention is applied to the detection of residual sulfur dioxide in white fungus, comprising the following steps:

[0050] (1) Draw a standard curve. With 490nm as the excitation wavelength, the concentration of the sulfite standard substance is determined to be 0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.1, and 0.2mM. The fluorescence intensity F of the standard solution at an emission wavelength of 520nm; Fluorescence intensity F at 520nm 0 , ΔF=F-F 0 , with the sulfite concentration C as the X-axis and ΔF as the Y-axis, draw a standard curve;

[0051] (2) Pulverize the sample Tremella to be tested with a grinder, and then accurately weigh 20.00g;

[0052] (3) Put the sample into the distillation bottle of the automatic distillation device, add 100mL of deionized water, place the absorption tube in 5mL of 5g / L sodium hydroxide absorption solution, and add 10mL of 1+1 hydrochloric acid ( Concentrated hydrochloric acid and deionized water volume ratio 1:1), quick...

Embodiment 3

[0056] The present invention is applied to the detection of residual sulfur dioxide in wine, comprising the following steps:

[0057] (1) Draw a standard curve. With 490nm as the excitation wavelength, the concentration of the sulfite standard substance is determined to be 0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.1, and 0.2mM. The fluorescence intensity F of the standard solution at an emission wavelength of 520nm; Fluorescence intensity F at 520nm 0 , ΔF=F-F0, with the sulfite concentration C as the X-axis, and ΔF as the Y-axis, a standard curve is drawn;

[0058] (2) Accurately absorb 20.00mL of the wine to be tested;

[0059] (3) Put the sample into the distillation bottle of the automatic distillation device, add 100mL of deionized water, place the absorption tube in 5mL of 5g / L sodium hydroxide absorption solution, and add 10mL of 1+1 hydrochloric acid ( Concentrated hydrochloric acid and deionized water volume ratio 1:1), quickly airtight and start the automatic distillatio...

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Abstract

The invention discloses a detection method for the residual quantity of sulfur dioxide in food and Chinese herbal medicines. The detection method comprises the following steps: pretreating of a sample; putting the sample into an automatic distillation apparatus, adding a hydrochloric acid solution, then rapidly sealing the automatic distillation apparatus, starting a distillation program, absorbing distillate with a sodium hydroxide solution and completing blank control testing at the same time; and adjusting the absorbed liquid with an acidic solution until the absorbed liquid is neutral, then adding a sulfite fluorescence probe, carrying out a reaction for 25 to 35 min, then placing the probe in a fluorospectrophotometer for measurement and quantifying the content of sulfur dioxide in the sample through a standard curvilinear equation. The detection method provided by the invention employs the automatic distillation apparatus to replace a conventional glass distillation apparatus, is simple and reliable, substantially saves distillation time and reduces system errors; the detection method broadens the scope of matrixes for application of the sulfite fluorescence probe to detection of the residual quantity of sulfur dioxide in food; and the sulfite fluorescence probe has specific recognition effect on sulfite radicals, so the situation of interference in traditional detection method is overcome, and reliability and accuracy of detection results are improved.

Description

technical field [0001] The patent of the present invention relates to a detection method of residual sulfur dioxide, specifically a detection method of residual sulfur dioxide in food and Chinese herbal medicine. Background technique [0002] As we all know, sulfur dioxide plays a pivotal role in food production and processing, and is often used as a bleaching agent and preservative. However, driven by profit, some unscrupulous businesses have serious abuse of sulfur dioxide in the process of food processing. [0003] Long-term intake of excessive sulfur dioxide by humans will cause serious damage to the body, including strong gastrointestinal irritation, induction of allergic diseases such as asthma, destruction of vitamin B1 in the body, and affecting the absorption of calcium and phosphorus elements. Therefore, my country's relevant food safety standards have clear regulations on the scope and limit of sulfur dioxide in food. [0004] At present, the detection of sulfur...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/31G01N1/28
CPCG01N21/6428G01N1/28G01N21/31
Inventor 马兴宓捷波高健会张海滨
Owner ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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