Method for rapidly detecting content of vitamin D3 in health foods

Abstract

The invention discloses a method for rapidly detecting the content of vitamin D3 in health foods. The method comprises the following steps: taking a sample to be detected; carrying out saponification and extraction to obtain a pre-treated sample solution; purifying the pre-treated sample solution by adopting a silica gel column solid-phase extraction and purification technology to obtain a sample solution to be detected; and identifying the vitamin D3 in the sample solution to be detected by adopting an ultra-high performance liquid chromatography technology to obtain the content of the vitamin D3 in the health foods. With the adoption of a solid-phase extraction-ultra-high performance liquid chromatography, a condition that a traditional high performance liquid chromatography needs a normal phase-reversed phase double-system operation method is changed, and the problems that pre-treatment time is long, effective components are easily damaged, the technical requirements on operators are high, the requirements on the quantity of laboratory instruments are great and the like are solved; after being pre-treated, the vitamin D3 is directly purified by a solid-phase extraction column and then is sampled in a reversed-phase liquid-phase system; and peaks are rapidly formed, the time is extremely shortened and the detection efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a rapid detection of vitamin D in health food 3 content method. Background technique [0002] Vitamin D 3 It is a sterol derivative, also known as cholecalciferol, which is mainly derived from dietary intake or synthesis in the body under ultraviolet radiation. It can promote the absorption of calcium and phosphorus in the body, and has a great effect on children's rickets and diseases caused by calcium deficiency in adults. Good preventative effect. Therefore, vitamin D 3 Often used in calcium-supplementing health foods. [0003] At present, in my country, for vitamin D in health food 3 The state has not yet provided a standard method for the detection of vitamin D content, but the demand for detection of health food is increasing day by day, and the detection methods for vitamin D mainly include enzyme-linked immunosorbent assay, high performance liquid chromatograp...

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Problems solved by technology

[0004] Among them, the enzyme-linked immunosorbent assay is mainly aimed at plasma samples, and its operation and equipment are relatively simple, but its scope of application is small, and it cannot detect health foods with complex matrices, such as beeswax products, fish oil products, etc.

[0005] High-performance liquid chromatography is mainly based on the method of GB5413.9-2010. This method is mainly aimed at infant food and dairy products. It uses a normal phase-reversed phase dual system for detection. The steps are cumbersome, and the operation time is long and easy to lose. The technical requirements and laboratory instrument conditions are very high. After obtaining the pretreated sample liquid, the operator needs to immediately enter the normal phase system to separate and collect the liquid within the required retention time period, and blow it with nitrogen immediately. The total processing time is too long It is very easy to destroy the content of vitamin

At the same time, the requirements for the laboratory are very high, and it is necessary to have two liquid phase instruments or an online switching device at the same time, and the cost is very high

[0006] High-performance liquid chromatography tandem mass spectrometry mainly uses isotope internal standard detection. This method has high sensitivity, but the isotope standard is expensive and is not suitable for the detection of a large number of samples in daily work. The price of purchasing isotope internal standard is much higher than the detection cost of general laboratories. times, and there is no domestic manufacturer, it needs to be purchased from abroad, and the waiting period is very long

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