Preparing method for fresh lumbricus extract

A technology of extract and earthworm, applied in the field of preparation of fresh earthworm extract, can solve the problems of few preparation methods of fresh earthworm extract, waste of medicinal materials, and improvement of extraction methods

Active Publication Date: 2016-03-23

云南永安制药有限公司 +1

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AI-Extracted Technical Summary

Problems solved by technology

[0012] There are not many methods for preparing the extract of Dilong Dilong, and the extract of Dilong Dilong obtained in the prior ...

Abstract

The invention relates to a preparing method for fresh lumbricus extract. The method comprises the following steps that 1, fresh lumbricus is thrown into a bone smashing machine to be smashed, and the fresh lumbricus smashed through the bone smashing machine is thrown into a bone paste mill to be mashed; 2, the mashed fresh lumbricus is added into an alkaline solution, and the mixture stands after being stirred; 3, an extracting solution after standing in the step 2 is filtered or centrifuged to obtain clear liquid; 4, the clear liquid is concentrated, an organic solution containing alcohol is added, the content of the alcohol in liquid medicine reaches 40-80% (v/v), and the liquid medicine stands after being stirred; 5, filtering or centrifuging is carried out to obtain precipitate, and the precipitate is dried to obtain the extract, wherein the organic solution containing the alcohol is selected from methyl alcohol, ethyl alcohol, propylene glycol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol and tertiary butanol, or a mixture of methyl alcohol, ethyl alcohol, propylene glycol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol and tertiary butanol, or a mixture of methyl alcohol, ethyl alcohol, propylene glycol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol and tertiary butanol and acetone.

Application Domain

Dispersion deliveryOrganic non-active ingredients +3

Technology Topic

ButanolIsopropyl alcohol +8

Image

Examples

Experimental program(9)

Example Embodiment

[0046] Example 1

[0047] Step 1, put 10kg of fresh ground dragon into the bone crusher to be broken, and then put the fresh ground dragon broken by the bone crusher into the bone mud grinding and beating;

[0048] Step 2, adding the 5% (w/w) sodium hydroxide solution of 5 times the weight of the fresh ground dragon after the beating, stirring and leaving standstill;

[0049] Step 3, the standing extract in step 1 is filtered or centrifuged to obtain clear liquid;

[0050] In step 4, the clear liquid is concentrated into a clear paste with a relative density of 1.02-1.06 (25° C.), and a mixed solution of ethanol and acetone with a concentration of 100% (v/v) (wherein the volume ratio of ethanol and acetone is 10:1) is added to make the solution. The alcohol content of the medicinal liquid is 55% (v/v), and after stirring, let it stand for precipitation;

[0051] Step 5: Filtration or centrifugation to remove the supernatant to obtain a precipitate, and after the precipitate is dried, 500 g of the fresh earthworm extract of the present invention is obtained.

Example Embodiment

[0052] Example 2

[0053] Step 1, put 10kg of fresh ground dragon into the bone crusher to be broken, and then put the fresh ground dragon broken by the bone crusher into the bone mud grinding and beating;

[0054]Step 2, adding the 5% (w/w) sodium hydroxide solution of 5 times the weight of the fresh ground dragon after the beating, stirring and leaving standstill;

[0055] Step 3, the standing extract in step 1 is filtered or centrifuged to obtain clear liquid;

[0056] In step 4, the clear liquid is concentrated into a clear paste with a relative density of 1.02-1.06 (25° C.), and a mixed solution of ethanol and acetone with a concentration of 95% (v/v) (wherein the volume ratio of ethanol and acetone is 5:1) is added to make the solution. The alcohol content of the medicinal liquid is 40% (v/v), after stirring, let it stand for precipitation;

[0057] Step 5: Filtration or centrifugation to remove the supernatant to obtain a precipitate, and after the precipitate is dried, 450 g of the fresh Dilong extract of the present invention is obtained.

Example Embodiment

[0058] Example 3

[0059] Step 1, put 10kg of fresh ground dragon into the bone crusher to be broken, and then put the fresh ground dragon broken by the bone crusher into the bone mud grinding and beating;

[0060] Step 2, adding the 5% (w/w) sodium hydroxide solution of 5 times the weight of the fresh ground dragon after the beating, stirring and leaving standstill;

[0061] Step 3, the standing extract in step 1 is filtered or centrifuged to obtain clear liquid;

[0062] In step 4, the clear liquid is concentrated into a clear paste with a relative density of 1.02-1.06 (25° C.), and a mixed solution of 95% (v/v) ethanol and acetone (wherein the volume ratio of ethanol and acetone is 15:1) is added to make the solution. The alcohol content of the medicinal liquid is 80% (v/v), after stirring, let it stand for precipitation;

[0063] Step 5: Filtration or centrifugation to remove the supernatant to obtain a precipitate, and after the precipitate is dried, 450 g of the fresh Dilong extract of the present invention is obtained.

[0064] After testing, the fresh Dilong extract of the present invention has the following characteristics

[0065] moisture

PUM

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