Preparing method for fresh lumbricus extract
A technology of extract and earthworm, applied in the field of preparation of fresh earthworm extract, can solve the problems of few preparation methods of fresh earthworm extract, waste of medicinal materials, and improvement of extraction methods
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Embodiment 1
[0047] Step 1, put 10kg of fresh earthworm into a bone crusher to break, and then put the fresh earthworm crushed by the bone crusher into a bone mud mill for beating;
[0048] Step 2, add 5% (w / w) sodium hydroxide solution of 5 times the weight to the fresh earthworm after beating, stir and let stand;
[0049] Step 3: Filtrate or centrifuge the extracted solution left standing in step 1 to obtain a supernatant;
[0050] Step 4, the clear liquid is concentrated into a clear paste with a relative density of 1.02 to 1.06 (25°C), and a mixed solution of ethanol and acetone with a concentration of 100% (v / v) (wherein the volume ratio of ethanol and acetone is 10:1) is added to make The alcohol content of the medicinal liquid is 55% (v / v), and it is left to stand after stirring until precipitation;
[0051] Step 5, filtering or centrifuging, removing the supernatant to obtain a precipitate, and drying the precipitate to obtain 500 g of the fresh earthworm extract of the present in...
Embodiment 2
[0053] Step 1, put 10kg of fresh earthworm into a bone crusher to break, and then put the fresh earthworm crushed by the bone crusher into a bone mud mill for beating;
[0054]Step 2, add 5% (w / w) sodium hydroxide solution of 5 times the weight to the fresh earthworm after beating, stir and let stand;
[0055] Step 3: Filtrate or centrifuge the extracted solution left standing in step 1 to obtain a supernatant;
[0056] Step 4, the clear liquid is concentrated into a clear paste with a relative density of 1.02 to 1.06 (25°C), and a mixed solution of ethanol and acetone with a concentration of 95% (v / v) (wherein the volume ratio of ethanol and acetone is 5:1) is added to make The alcohol content of the medicinal solution is 40% (v / v), and it is left to stand after stirring until precipitation;
[0057] Step 5, filtering or centrifuging, removing the supernatant to obtain a precipitate, and drying the precipitate to obtain 450 g of the fresh earthworm extract of the present inv...
Embodiment 3
[0059] Step 1, put 10kg of fresh earthworm into a bone crusher to break, and then put the fresh earthworm crushed by the bone crusher into a bone mud mill for beating;
[0060] Step 2, add 5% (w / w) sodium hydroxide solution of 5 times the weight to the fresh earthworm after beating, stir and let stand;
[0061] Step 3: Filtrate or centrifuge the extracted solution left standing in step 1 to obtain a supernatant;
[0062] Step 4, the supernatant is concentrated into a supernatant with a relative density of 1.02 to 1.06 (25° C.), and a mixed solution of ethanol and acetone with a concentration of 95% (v / v) (wherein the volume ratio of ethanol and acetone is 15:1) is added to make The alcohol content of the liquid medicine is 80% (v / v), and it is allowed to stand still after stirring, and to be precipitated;
[0063] Step 5, filtering or centrifuging, removing the supernatant to obtain a precipitate, and drying the precipitate to obtain 450 g of the fresh earthworm extract of th...
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