Stable serum-potassium detection reagent with high anti-interference capability and detection method

A technology for detection reagents and detection methods, which is applied in the field of serum potassium detection, can solve the problems of poor stability, susceptibility to interference, and high cost, and achieve the effects of enhancing stability, convenient use, and preventing turbidity

Inactive Publication Date: 2016-03-23
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the electrodes of this method have a certain lifespan and need to be replaced regularly, resulting in higher costs.
The precision and accuracy of the enzymatic method can be close to that of the flame photometric method, and it has the advantages of simplicity, accuracy, good repeatability, strong specificity, and less serum consumption, but has the disadvantages of poor stability and susceptibility to interference.

Method used

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  • Stable serum-potassium detection reagent with high anti-interference capability and detection method
  • Stable serum-potassium detection reagent with high anti-interference capability and detection method
  • Stable serum-potassium detection reagent with high anti-interference capability and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Detection reagents for serum potassium, including reagent R1 and reagent R2:

[0056] 1) The composition of its R1 is:

[0057] Tris (trishydroxymethylaminomethane) buffer (pH=8.2, 25°C) ·························· 200mmol / L

[0058] Acupoint Mixture ····································································································· 14mmol / L,

[0059] PEPs ·········································································································· 4mmol / L,

[0060] ADP ········································································································· 3.5mmol / L,

[0061] formic acid ········································································································· 0.2mmol / L,

[0062] formate dehydrogenase ······························································································· 100U / L,

[0063] alpha-ketoglutarate ·················································...

Embodiment 2

[0090] Interference test: Take fresh mixed serum, divide it into 2 equal parts, and then divide each equal part into 4 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then respectively use the reagent obtained in Example 1, and compare the content of K in serum with the common and approved serum potassium (K) reagent in the market simultaneously. The measured results of the control group and the measured results of each group after adding different interfering substances are shown in Table 2. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0091] It can be seen from Table 2 that the reagent of Example 1 has no obvious interference on the test results when bilirubin≤665μmol / L, triglyceride≤24.2mmol / L, and hemoglobin≤1g / L. However, the reagents of the control group were significantly inte...

Embodiment 3

[0095] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with the serum potassium kit of a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are shown in Table 3 Show. And obtained the correlation curve of the two reagents (such as figure 1 As shown), the test results show that the correlation coefficient of the two kits is 0.9996, which shows that there is a great correlation between the two.

[0096] Table 3 Example 1 reagent and market common and recognized serum potassium determination kit comparative detection results

[0097]

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Abstract

The invention relates to the technical field of serum potassium detection, in particular to a serum-potassium detection reagent. A reagent R1 is prepared from a buffer solution, a cryptate agent, PEP, ADP, formic acid, formate dehydrogenase, alpha-ketoglutaric acid, NADH, GLDH (glutamate dehydrogenase), polyethylene glycol 6000, ethylene glycol, mannitol, trehalose, BSA, PK (pyruvate kinase), alkyl polyglucoside (APG) and preservative; a reagent R2 is prepared from a buffer solution, polyethylene glycol 6000, ethylene glycol, mannitol, trehalose, BSA, LDH (lactic dehydrogenase), alkyl polyglucoside (APG) and preservative. The Tris buffer solutions are adopted, multiple stabilizers are added, an NADH cyclic regeneration system is introduced, and therefore the stability of the reagent is remarkably improved; as the novel nonionic surfactant alkyl polyglucoside (APG) is added, turbidity of the reaction system can be effectively prevented, and the sensitivity and the stability of the reagent are remarkably improved.

Description

technical field [0001] The invention relates to the technical field of serum potassium detection, in particular to a serum potassium detection reagent and a detection method using the detection reagent. Background technique [0002] Potassium (K) is the main cation to maintain the physiological activities of cells in the human body. It plays an important role in maintaining the normal osmotic pressure and acid-base balance of the body, participating in sugar and protein metabolism, and ensuring the normal function of neuromuscular. Most of the potassium ions (98%) exist in the cells, and a small amount exists in the extracellular fluid, and the concentration is constant. Normal human serum contains K + About 4~5mmol / L. Potassium ions in the body are constantly exchanged between cells and body fluids to maintain a dynamic balance. Determination of serum potassium, sodium, and chlorine is one of the common clinical combined detection items, which is helpful for the judgment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/32
CPCC12Q1/48C12Q1/32
Inventor 李志明谭柏清李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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