Human anti-mullerian hormone gold immunochromatographic detection kit and method thereof

An immunochromatographic detection and Müllerian tube technology, which is applied in the field of colloidal gold immunochromatographic rapid detection, can solve the problems of high price, false positives and high background value, and achieve the effects of short detection time, easy portability and simple operation.

Inactive Publication Date: 2016-04-27
NANJING MUMEI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional European and American detection method is the method of enzyme-linked immunoassay AMH kit, but it is time-consuming, low sensitivity, high background value, easy to cause false positive and false negative results, and requires professional personnel to perform experimental operations
Therefore, methods such as chemiluminescence are gradually replaced at present, but such methods, such as Roche's ELECSYSAMH detector, are still closed systems, expensive, and require special training for instrument users, high maintenance and testing costs, and are not suitable for promotional use
The above are the main methods for detecting AMH in European and American countries. At present, there are no kits for detecting AMH with independent intellectual property right

Method used

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  • Human anti-mullerian hormone gold immunochromatographic detection kit and method thereof
  • Human anti-mullerian hormone gold immunochromatographic detection kit and method thereof
  • Human anti-mullerian hormone gold immunochromatographic detection kit and method thereof

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Embodiment

[0049] Example: such as Figure 1-6 As shown, the present invention provides a first embodiment of a human anti-Müllerian hormone colloidal gold immunochromatography detection kit, which includes: a reagent cartridge, a bottom plate arranged in the reagent cartridge, and a sample pad arranged at one end of the bottom plate , a colloidal gold pad with one end in contact with the sample pad, a detection pad with one end in contact with the other end of the colloidal gold pad, and an absorbent pad in contact with the other end of the detection pad. The colloidal gold pad contains colloidal gold nanoparticles and the first antibody combined with mouse anti-human AMH, also known as immune colloidal gold solution. On the detection pad, non-overlapping detection lines and quality control lines are distributed, wherein the detection line contains the second antibody of mouse anti-human AMH, and the quality control line contains the third antibody against the first antibody. Wherein t...

no. 2 example

[0058] The present invention provides a second embodiment of a colloidal gold immunochromatographic detection method for human anti-Müllerian hormone, which includes:

[0059] 1. Preparation steps of colloidal gold nanoparticles

[0060] The chloroauric acid solution is reduced into colloidal gold nanoparticles with a particle diameter of 10-100 nm. For example, to prepare 40nm colloidal gold particles: take 100mL of chloroauric acid solution with a concentration of 0.01%, put it in a clean reflux round bottom flask, heat it in a reflux oil bath at a constant temperature of 200-250°C and stir at a low speed for 30min, until it reaches boiling state, quickly once Add 1.5 mL of 1% trisodium citrate solution, continue to reflux at a constant temperature, stir at a high speed, and keep boiling. The solution was light yellow-black-purple-wine red. After the color became stable, it was stirred at a low speed, kept for 10 min, and cooled to room temperature to obtain colloidal gold ...

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Abstract

The invention discloses a human anti-mullerian hormone gold immunochromatographic detection kit and a method thereof. The detection kit comprises a reagent clamping shell, a baseboard arranged inside the reagent clamping shell, a sample pad disposed at one end of the baseboard, a colloidal gold pad with one end pressing against the sample pad, a detection pad with one end pressing against the other end of the colloidal gold pad and an absorption pad contacted with the other end of the detection pad, wherein the colloidal gold pad contains colloidal gold nanoparticles and a first antibody bound to mouse-anti-human AMH, and a detection line and a quality control line which are misaligned are distributed on the detection pad. The detection line contains a second mouse-anti-human AMH antibody, and the quality control line contains a third anti-first-antibody antibody. According to the invention, concentration of AMH in female serum can be rapidly and instantly detected so as to be used in evaluating activity and storing capacity of ovums in a woman's ovary. The detection kit is accurate and fast and has high sensitivity. No professionals are needed to operate the kit. In addition, the kit is low-cost and is convenient to promote and use.

Description

technical field [0001] The invention relates to the field of rapid detection of colloidal gold immunochromatography, in particular to a colloidal gold immunochromatographic detection kit and method for human anti-Müllerian hormone. Background technique [0002] Anti-Müllerian hormone (AMH), as a new indicator for evaluating women's ovarian function, compared with the traditional evaluation of follicle stimulating hormone (FSH) level, inhibin B (INH) level and number of ovarian follicles, etc. As an indicator of ovarian function, AMH remains constant during the menstrual cycle of the tester, and the level of serum AMH does not change with the fluctuation of gonadal hormones. Therefore, the detection of AMH is not restricted by the female menstrual cycle and can be detected at any time. One of the main problems faced by obstetrics and reproductive departments. AMH is a dimeric glycoprotein formed by two identical 70KD subunits through a disulfide bond. In women, AMH is direct...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/558
CPCG01N33/74G01N33/558
Inventor 陈明慧郝睿
Owner NANJING MUMEI BIOTECH CO LTD
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