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Betulinic acid nano-liposome wrapped with nanogold spherical shell and preparation method thereof

A nano-liposome and betulinic acid technology, which is applied in the field of medicine, can solve the problems of capture targeting, poor stability, and easy aggregation, and achieve the effects of easy control of the reaction, high repeatability, and simple operation

Active Publication Date: 2016-05-04
秦皇岛满药本草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, conventional nanoliposomes have a series of defects, such as poor stability (easy to agglomerate, precipitate), easy to be captured by the phagocytic system of monocytes, and poor targeting.

Method used

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  • Betulinic acid nano-liposome wrapped with nanogold spherical shell and preparation method thereof
  • Betulinic acid nano-liposome wrapped with nanogold spherical shell and preparation method thereof
  • Betulinic acid nano-liposome wrapped with nanogold spherical shell and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 6mg betulinic acid (purchased from Nanjing Zelang Biotechnology Co., Ltd.), 5.4mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 54mg soy lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.), in Under magnetic stirring, it was dissolved in 5 mL of absolute ethanol to obtain a lipid solution. Dissolve 8 mg of glutathione in 20 mL of deionized water to obtain a glutathione solution. Under magnetic stirring, drop 5 mL of lipid solution slowly and uniformly into 10 mL of glutathione solution at 36° C. to obtain liposome suspension. Place it on a shaker, at 26° C., at 110 rpm, for 1 hour to remove the absolute ethanol in the liposome suspension to obtain glutathione-modified betulinic acid nanoliposomes.

[0026] By chemical reduction method, 5μL, 240mM sodium borohydride (NaBH 4 ) The solution was quickly added dropwise to 1mL 0.2mM gold chloride solution, and the dropwise addition was shaken and mixed until the color chan...

Embodiment 2

[0030] 6mg betulinic acid (purchased from Nanjing Zelang Biotechnology Co., Ltd.), 7.2mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 72mg soy lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.), in Under magnetic stirring, it was dissolved in 5 mL of absolute ethanol to obtain a lipid solution. 16 mg of glutathione was dissolved in 20 mL of deionized water to obtain a glutathione solution. Under magnetic stirring, drop 5 mL of lipid solution slowly and uniformly into 15 mL of glutathione solution at 40° C. to obtain liposome suspension. Place it on a shaker, remove the absolute ethanol in the liposome suspension at 28° C., 120 rpm, and 1.5 hours to obtain glutathione-modified betulinic acid nanoliposomes.

[0031] By chemical reduction method, 18μL, 250mM sodium borohydride (NaBH 4 ) The solution is quickly added dropwise to 1mL, 0.6mM gold chloride solution, and shake and mix while dropping until the color becomes orange...

Embodiment 3

[0035] 6mg betulinic acid (purchased from Nanjing Zelang Biotechnology Co., Ltd.), 9mg cholesterol (purchased from Tianjin Damao Chemical Instrument Supply Station) and 90mg soy lecithin (purchased from Shenyang Tianfeng Biopharmaceutical Co., Ltd.), Under stirring, dissolve in 5 mL of absolute ethanol to obtain a lipid solution. Dissolve 24 mg of glutathione in 20 mL of deionized water to obtain a glutathione solution. Under magnetic stirring, drop 5 mL of lipid solution slowly and uniformly into 20 mL of glutathione solution at 45° C. to obtain liposome suspension. Place it on a shaker at 30° C., 140 rpm, and remove the absolute ethanol in the liposome suspension for 2.5 hours to obtain glutathione-modified betulinic acid nanoliposomes.

[0036] By chemical reduction method, 30μL, 260mM sodium borohydride (NaBH 4 ) The solution was quickly added dropwise to 1 mL, 1.0 mM gold chloride solution, and mixed with shaking while dropping until the color changed to orange-red, that is...

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Abstract

A betulinic acid nano-liposome wrapped with a nanogold spherical shell is a nano drug-loading system with the particle size being 130-200 nm, a layer of nanogold spherical shell is formed on the surface of the betulinic acid nano-liposome modified by glutathione, and the surface plasma resonant absorption wavelength ranges from 750 nm to 850 nm. A preparation method of the betulinic acid nano-liposome mainly includes the steps that glutathione serves as a raw material to prepare the betulinic acid nano-liposome, and the surface of the betulinic acid nano-liposome has mercapto functional groups; the liposome and gold nanoparticles are inoculated under mild conditions, then a gold chloride solution is added, the mixture stands still for 15-40 min at room temperature and then placed in ice bath, and finally a sodium borohydride solution is added for inoculation for 4-9 h. The method can be implemented under normal-temperature, normal-pressure and mild conditions, operation is easy, and reaction is easy to control. The prepared betulinic acid nano-liposome wrapped with the nanogold spherical shell has good photo-thermal conversion performance, and release is controllable.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to a nano liposome and a preparation method thereof. Background technique [0002] In today's society, cancer has become the second killer of human health, and the cure of cancer has always been a major problem in the world. Chemotherapy is currently the first choice for the treatment of many cancers. However, anti-tumor chemotherapeutic drugs have many defects, such as severe toxic and side effects on the body, low drug concentration in tumor sites, and drug resistance caused by long-term treatment. Therefore, the current problem to be solved is to find new therapeutic drugs that can destroy tumor cells. [0003] Studies have found that natural plant anti-tumor drugs have significant anti-cancer effects and small adverse reactions, and have gradually become the protagonist in the anti-tumor drug market. Betulinic acid is a five-ring triterpenoid compound widely distributed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K9/51A61K47/18A61K31/56A61K41/00
CPCA61K9/0002A61K9/127A61K9/5115A61K31/56A61K41/0052A61K47/183A61K2300/00
Inventor 高大威刘艳平张旭武骆丽垚
Owner 秦皇岛满药本草生物科技有限公司
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