Leech small peptide chelate with coagulation preventing and blood activating effect and preparation method thereof

A small peptide chelate and leech technology, applied in the biological field, can solve problems such as vision and dark adaptation effects, vitamin A can not normally play a physiological role, and achieve good economic benefits, low cost, and easy preparation

Inactive Publication Date: 2016-05-04
ANHUI BIOLOGICAL PEPTIDE IND RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When zinc is deficient, vitamin A cannot normally play a physiological role, which affects vision and dark adaptation

Method used

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  • Leech small peptide chelate with coagulation preventing and blood activating effect and preparation method thereof
  • Leech small peptide chelate with coagulation preventing and blood activating effect and preparation method thereof
  • Leech small peptide chelate with coagulation preventing and blood activating effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Crush the leeches through an 80-mesh sieve, add 12 times the amount of water to boil, wait until cooled to about 40°C, adjust the pH value to 2.0, add 1% pepsin, keep warm for 1 hour, adjust the pH value to 8.0, add 1% trypsin, and keep warm for 3 hour, boiled for 15 minutes, centrifuged, the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, and the permeate was added to a DA201-C macroporous adsorption resin column, eluted with water, and the water wash was discarded, and then Elute with 90% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a clear paste with a relative density of 1.10-1.15 at 60°C, spray dry (inlet air temperature is 180-200°C, outlet air temperature is 90 ~100°C) to obtain leech small peptide extract.

[0067] Dissolve copper gluconate in distilled water to make a salt solution with a concentration of 15 mg / ml, adjust the pH value to 3 with...

Embodiment 2

[0069] Crush the leeches through an 80-mesh sieve, add 10 times the amount of water to boil, wait until cooled to about 45°C, adjust the pH value to 1.5, add 2% pepsin, keep warm for 2 hours, adjust the pH value to 9.0, add 2% trypsin, and keep warm for 4 hour, boiled for 15 minutes, centrifuged, the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, and the permeate was added to a DA201-C macroporous adsorption resin column, eluted with water, and the water wash was discarded, and then Elute with 75% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a clear paste with a relative density of 1.10-1.15 at 60°C, spray dry (inlet air temperature is 180-200°C, outlet air temperature is 90 ~100°C) to obtain leech small peptide extract.

[0070] Dissolve zinc gluconate in distilled water to make a salt solution with a concentration of 10 mg / ml, adjust the pH value to 4 with ...

Embodiment 3

[0072] Get the leech small peptide chelate 100g that embodiment 1 method gained, by leech small peptide chelate: corn oil: beeswax: lecithin ratio is 10:36:3:1 mixed material, grinds 30 minutes through colloid mill, with Gelatin, glycerin, purified water, and iron oxide black are used to make soft capsule skin glue at a ratio of 100:40:100:1. The temperature of the plastic box is controlled at 55±5°C, the temperature of the heat preservation tank is 40±5°C, and the temperature of the spray body is 36±5°C. , press soft capsules with a pill press, the content of each capsule weighs 0.50g, dry in cold air at 18°C ​​to 26°C for 4 hours (relative humidity is 30% to 40%) to shape, wash, dry, select pills, pack, and prepare into soft capsules. Capsule finished product.

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Abstract

The invention discloses a leech small peptide chelate with a coagulation preventing and blood activating effect and a preparation method thereof, and belongs to the field of biotechnology. The preparation method is characterized in that with leeches as a starting material, enzymolysis, ultrafiltration, resin column purification, a chelation reaction and drying are carried out to obtain the leech small peptide chelate, and preparation accessories can be added to prepare the leech small peptide chelate into a corresponding preparation through a conventional method. The leech small peptide chelate has a strong coagulation preventing and blood activating effect and has the advantages of being free of toxic and side effects and drug misadventures, low in cost and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a small leech peptide chelate with anticoagulant and blood-activating effects and a preparation method thereof. Background technique [0002] Leech is the dried whole body of leeches WhitmaniapigraWhitman, HirudonipponicaWhitman, and willow-leaf leech Whitmania acranulataWhitman, which is a traditional Chinese medicine. Leeches were first recorded in "Shen Nong's Materia Medica": "(leech) mainly drives away bad blood, blood stasis, moon closure, accumulation of bloody lumps, no children, and water passages." The saltiness and bitterness of leech, in order to remove blood accumulation, is the medicine of the liver meridian blood, so it can clear the liver meridian and gather blood." Traditional Chinese medicine believes that leech has the effects of breaking blood, removing blood stasis, and dredging menstruation. It is mainly used to treat lumps, lumps, Blood stasis, amenorrhea and ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/16A61K38/01A61K35/62A61P7/02A61K8/98A61K8/64A61Q19/00
CPCC07K7/06A61K8/64A61K8/987A61K38/00A61Q19/00C12P21/06
Inventor 卢宁代龙凌中鑫
Owner ANHUI BIOLOGICAL PEPTIDE IND RES INST
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