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Biological DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) room-temperature preservation card and manufacturing method thereof

A production method and technology for storing cards, which are applied in the biological field and can solve the problems of high energy consumption, high cost and high transportation cost

Inactive Publication Date: 2016-05-18
GANNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to high energy consumption and high cost during conventional storage, dry ice protection is required during transportation, resulting in high transportation costs

Method used

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  • Biological DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) room-temperature preservation card and manufacturing method thereof
  • Biological DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) room-temperature preservation card and manufacturing method thereof
  • Biological DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) room-temperature preservation card and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Take 1MTris-HCl (pH8.0) 10mL, 0.5MEDTA (pH8.0) 2mL, add ultrapure water to make up to 1L, after autoclaving, cool to room temperature, add 35mL hydrogen peroxide, mix well, put into cutting Soak good filter paper, drying box, tweezers and creasing tube overnight;

[0025] (2) Drain the soaked material, put it in a drying box, and dry it in an oven at 80°C;

[0026] (3) Press out six circular rings with a diameter of 5 mm in the middle of the filter paper with an indentation tube, and stick the dry filter paper to the left end of the card with the sample information filled in with tweezers;

[0027] (4) Put 10 μL of extracted citrus greening disease leaf sample DNA (concentration 1500-5000ng / μL) in the middle of the circular indentation with a pipette gun, insert each card vertically into the card slot of the drying box, and place Quickly dehydrate and dry at room temperature in a desiccator with color-changing silica gel;

[0028] (5) Cover the end with filter pap...

Embodiment 2

[0032] (1) Take 1.21g TrisBase and 0.564gNa 2 EDTA, add ultrapure water to dissolve, adjust pH to 8.0 with 1M HCl, add ultrapure water to 1L, cool to room temperature after autoclaving, add 40mL hydrogen peroxide, mix well, put in the cut nitrocellulose membrane, dry Boxes, tweezers, creasing tubes and plastic wrap overnight;

[0033] (2) Drain the soaked material, put it in a drying box, and dry it in an oven at 80°C;

[0034] (3) Press out six circular rings with a diameter of 5 mm in the middle of the nitrocellulose membrane with an indentation tube, and stick the dried nitrocellulose membrane to the transparent area at the left end of the filled sample information card with tweezers;

[0035](4) Use a pipette gun to place 10 μL of extracted citrus vein yellow leaf sample RNA (concentration 1500-2500ng / μL) in the middle of the circular indentation, and insert the cards vertically into the card slots of the drying box, Placed with melaleuca-type coumarate (ie, melaleuca-21...

Embodiment 3

[0040] (1) Take 10mL of 1MTris-HCl (pH8.0), 2mL of 0.5MEDTA (pH8.0), add ultrapure water to make up to 1L, cool to room temperature after autoclaving, add 30mL of hydrogen peroxide, mix well, put into the cutting Soak the good silicone membrane, drying box, tweezers and creasing tube overnight;

[0041] (2) Drain the soaked material, put it in a drying box, and dry it in an oven at 80°C;

[0042] (3) Press out six circular rings with a diameter of 5mm in the middle of the silicone membrane with an indentation tube, and stick the dry silicone membrane to the transparent area at the left end of the filled sample information card with tweezers;

[0043] (4) Put 10 μL of the extracted pEasy-colicin plasmid DNA (concentration 600-2000ng / μL) in the middle of the circular indentation with a pipette gun, insert the cards vertically into the card slots of the drying box, and place the Rapid dehydration and drying at room temperature in an anhydrous calcium sulfate desiccator;

[0044...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a biological DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) room-temperature preservation card and a manufacturing method thereof. The sample preservation medium of the preservation card is a material with water absorptivity or DNA combining capacity. The sample preservation medium contains a nuclease inhibitor and a nucleic acid stabilizer, and is subjected to DNase and RNase removal treatment. Compared with the prior art, the biological DNA and RNA room-temperature preservation card has the following advantages: the material with water absorptivity is adopted to preserve the dry DNA onto a film, and the film is subjected to enzyme removal treatment and treated by the nucleic acid stabilizer, so that the DNA and RNA can be preserved for a long time without degradation; the preservation card is free of toxic and harmful substances, and thus, is safe for the human and environment; all the used reagents and materials are free of PCR (polymerase chain reaction) and other enzymatic reaction inhibition components, and are suitable for the development of all downstream experiments in molecular biology; the preservation card does not need solid carbon dioxide in the transportation or mailing process, and can be transported at normal temperature; and the card information is detailed and is convenient for classified storage and search.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a biological DNA and RNA room temperature preservation card and a manufacturing method thereof. Background technique [0002] The genetic traits of organisms are mainly determined by nucleic acids, which include DNA and RNA. In recent years, with the development of molecular biology and various omics technologies, the comparative study of nucleic acid sequences has gradually become a basic means for disease diagnosis, genetic variation, regulatory mechanism and disease epidemic analysis. For the preservation of a large number of DNA and RNA resources, it is of great significance to develop a simple method for sorting, long-term storage and transportation of important sample nucleic acids. [0003] At present, the common nucleic acid preservation method is mainly to freeze the nucleic acid solution at -20°C for short-term storage, and to store it at -80°C or liquid nitrog...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/10C12Q2527/125C12Q2527/127
Inventor 苏华楠易龙钟八莲卢占军黄爱军
Owner GANNAN NORMAL UNIV
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