Method for separating Sclerotinia homoeocarpa

Abstract

The invention discloses a method for separating Sclerotinia homoeocarpa. The method comprises the following steps: collection of disease samples: cutting off a diseased and healthy junction part of a plant having a typical dollar spot symptom and a soil sample as the disease samples; moisturized culture of the disease samples; pathogenic fungi separation: using a transfer needle to pick up filament-shaped spawn growing on the surfaces of the disease samples, putting the filament-shaped spawn on an APDA culture medium, and carrying out culture for 2 days under a dark condition at 22 to 25 DEG C; pathogenic fungi purification: after aerial hyphae grow out, cutting tips of the aerial hyphae on the edge of abacterial colony, putting the tips onto a new APDA culture medium, and carrying out culture for 3 to 5 daysunder a dark condition at 22 to 25 DEG C, so as to obtain target pathogenic bacteria. As moisturized culture pretreatment is conducted on the disease samples, and the acidized PDA culture medium is used, the problem of various non-target fungus contamination in a Sclerotinia homoeocarpa separation process can be solved; the operation is simple; the whole process is free of strict disinfection and sterilization operation. Compared with the traditional Sclerotinia homoeocarpa separation method, the separation efficiency and the success rate of the method disclosed by the invention are improved obviously.

Description

technical field [0001] The invention belongs to the field of plant pathogenic microorganisms, and relates to a method for isolating turf coin spot bacteria. Background technique [0002] Sclerotinia homoeocarpa is an important plant pathogenic fungus, which has a variety of hosts and can infect a variety of cool-season and warm-season turfgrasses. Since it was first discovered and reported in the United States in 1932, it has now spread widely all over the world. Preliminary survey results show that turf dollar spot has occurred in more than 20 provinces and autonomous regions in my country. Dollar spot disease mainly harms the blades of turfgrass, causing the turfgrass to turn yellow and even die, seriously affecting the landscape beauty of the lawn. [0003] Dollar spot disease mainly has two peaks in early summer and early autumn. When the soil temperature is higher than 18°C ​​and the humidity is high at night, dollar spot disease begins to develop. At the early stage ...

AI Technical Summary

Problems solved by technology

In the late stage of infection, the infected lawn leaves are often mixed with a variety of saprophytic fungi or bacteria, so it is often difficult to quickly and accurately isolate the target bacteria in the actual isolation process of dollar spot bacteria

[0005] Conventional coin spot bacteria isolation has strict requirements on the time from collection of disease samples to isolation. Usually, pathogenic bacteria need to be isolated within 24 hours of collection to improve the success rate of isolation. However, in the actual operation process, disease samples need to be transported over long distances after collection Only in the laboratory can the isolation of pathogenic bacteria be carried out. At this time, other pathogenic bacteria are easy to breed, thereby reducing the success rate of target bacteria isolation; in addition, conventional isolation methods require strict disinfection of diseased tissues, and disinfection may occur at the same time. Will kill the target bacteria, resulting in greatly reduced isolation efficiency of pathogens

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