A plant fermented product of Saccharomyces cerevisiae and its application and skin external preparation
A skin topical agent, the technology of Saccharomyces cerevisiae, applied in the field of microorganisms, can solve the problems of antioxidant performance barriers, etc., and achieve the effect of improving the ability to scavenge free radicals, delaying skin aging, and reducing damage
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Embodiment 1
[0039] Example 1 Preparation of Fermented Saccharomyces cerevisiae and Ginseng
[0040] (1) Preparation of ginseng extract
[0041] (1) Preparation of ginseng flower extract
[0042] Crush 200g of commercially available ginseng flower, add deionized water at a ratio of 1:10 (g / mL), stir and extract 2 times at 90°C in a water bath for 60 minutes each time. The filtrate after solid-liquid separation is added to the filtrate. Remove the water to obtain the ginseng flower extract.
[0043] (2) Preparation of ginseng root extract
[0044] Crush 200g of commercially available ginseng root, add deionized water at a ratio of 1:15 (g / mL), stir and extract 4 times in a water bath at 80°C for 90 minutes each time, the filtrate after solid-liquid separation, remove the water from the filtrate It is ginseng root extract.
[0045] (3) Preparation of ginseng seed extract
[0046] Crush 200g of commercially available ginseng seeds, add deionized water at a ratio of 1:20 (g / mL), stir and extract twice a...
Embodiment 2
[0063] Example 2 Detection of Antioxidant Activity of Fermented Saccharomyces cerevisiae and Ginseng
[0064] The Saccharomyces cerevisiae ginseng flower fermentation product, Saccharomyces cerevisiae ginseng root extract, Saccharomyces cerevisiae ginseng seed extract, and Saccharomyces cerevisiae whole ginseng fermentation product prepared in steps (2) (1) to (4) of Example 1 were implemented; Example 1 The ginseng flower extract, ginseng root extract, ginseng seed extract and whole ginseng extract prepared in step (1) (1) to (4) were used to determine the antioxidant activity of scavenging free radicals by DPPH method .
[0065] The determination steps are: prepare the above-mentioned sample to be tested with deionized water to prepare 5mg / mL solution A, pipette 2mL solution A into a 10mL test tube with a stopper, and add 2mL 2×10 -4 mol / L DPPH ethanol solution (DPPH purchased from Sinopharm Chemical Reagent Co., Ltd.), mix well, and let stand at room temperature for 30 minutes, ...
Embodiment 3
[0070] Example 3 Preparation of fermented product of Saccharomyces cerevisiae Saussurea
[0071] The strain DZ-49 was inoculated into PDA liquid medium and cultured at 28°C for 48 hours to obtain seed liquid; among them, PDA liquid medium: 5g / L potato powder, 15g / L glucose, 15g / L peptone and 5g / L NaCl.
[0072] Take the commercially available snow lotus, crush it to a coarse powder with a particle size of about 100 mesh, add 20 times the volume of deionized water to ultrasonically extract twice for 1 hour each time, combine the filtrate and concentrate to dryness to obtain the snow lotus extract.
[0073] Inoculate 5% (v / v) seed liquid into a PDA liquid medium containing 10% (w / w) Saussurea extract, culture at 28° C. and 200 rpm shaker for 5 days to obtain a culture liquid. The culture broth was centrifuged at 8°C and 8000 rpm for 5 minutes, and the supernatant was taken to obtain the fermented product of Saccharomyces cerevisiae Saussurea.
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