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A plant fermented product of Saccharomyces cerevisiae and its application and skin external preparation

A skin topical agent, the technology of Saccharomyces cerevisiae, applied in the field of microorganisms, can solve the problems of antioxidant performance barriers, etc., and achieve the effect of improving the ability to scavenge free radicals, delaying skin aging, and reducing damage

Active Publication Date: 2019-10-08
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Loss of NAD(H) kinase will lead to impairment of cellular antioxidant performance

Method used

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  • A plant fermented product of Saccharomyces cerevisiae and its application and skin external preparation
  • A plant fermented product of Saccharomyces cerevisiae and its application and skin external preparation
  • A plant fermented product of Saccharomyces cerevisiae and its application and skin external preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of Fermented Saccharomyces cerevisiae and Ginseng

[0040] (1) Preparation of ginseng extract

[0041] (1) Preparation of ginseng flower extract

[0042] Crush 200g of commercially available ginseng flower, add deionized water at a ratio of 1:10 (g / mL), stir and extract 2 times at 90°C in a water bath for 60 minutes each time. The filtrate after solid-liquid separation is added to the filtrate. Remove the water to obtain the ginseng flower extract.

[0043] (2) Preparation of ginseng root extract

[0044] Crush 200g of commercially available ginseng root, add deionized water at a ratio of 1:15 (g / mL), stir and extract 4 times in a water bath at 80°C for 90 minutes each time, the filtrate after solid-liquid separation, remove the water from the filtrate It is ginseng root extract.

[0045] (3) Preparation of ginseng seed extract

[0046] Crush 200g of commercially available ginseng seeds, add deionized water at a ratio of 1:20 (g / mL), stir and extract twice a...

Embodiment 2

[0063] Example 2 Detection of Antioxidant Activity of Fermented Saccharomyces cerevisiae and Ginseng

[0064] The Saccharomyces cerevisiae ginseng flower fermentation product, Saccharomyces cerevisiae ginseng root extract, Saccharomyces cerevisiae ginseng seed extract, and Saccharomyces cerevisiae whole ginseng fermentation product prepared in steps (2) (1) to (4) of Example 1 were implemented; Example 1 The ginseng flower extract, ginseng root extract, ginseng seed extract and whole ginseng extract prepared in step (1) (1) to (4) were used to determine the antioxidant activity of scavenging free radicals by DPPH method .

[0065] The determination steps are: prepare the above-mentioned sample to be tested with deionized water to prepare 5mg / mL solution A, pipette 2mL solution A into a 10mL test tube with a stopper, and add 2mL 2×10 -4 mol / L DPPH ethanol solution (DPPH purchased from Sinopharm Chemical Reagent Co., Ltd.), mix well, and let stand at room temperature for 30 minutes, ...

Embodiment 3

[0070] Example 3 Preparation of fermented product of Saccharomyces cerevisiae Saussurea

[0071] The strain DZ-49 was inoculated into PDA liquid medium and cultured at 28°C for 48 hours to obtain seed liquid; among them, PDA liquid medium: 5g / L potato powder, 15g / L glucose, 15g / L peptone and 5g / L NaCl.

[0072] Take the commercially available snow lotus, crush it to a coarse powder with a particle size of about 100 mesh, add 20 times the volume of deionized water to ultrasonically extract twice for 1 hour each time, combine the filtrate and concentrate to dryness to obtain the snow lotus extract.

[0073] Inoculate 5% (v / v) seed liquid into a PDA liquid medium containing 10% (w / w) Saussurea extract, culture at 28° C. and 200 rpm shaker for 5 days to obtain a culture liquid. The culture broth was centrifuged at 8°C and 8000 rpm for 5 minutes, and the supernatant was taken to obtain the fermented product of Saccharomyces cerevisiae Saussurea.

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Abstract

The invention discloses a saccharomyces cerevisiae plant fermentation product and application thereof and skin external application preparations. The saccharomyces cerevisiae plant fermentation product is obtained by culturing saccharomyces cerevisiae CCTCC NO: M 2015725 on a culture medium containing a plant extract. The saccharomyces cerevisiae plant fermentation product has the advantages of being resistant to oxidation and aging and can be applied to preparation of the skin external application preparations. The prepared skin external application preparations have the excellent antioxidant property and anti-aging property. The skin external application preparations can effectively clear free radicals, reduce damage to skin due to light aging and oxidative stress and delay skin aging.

Description

Technical field [0001] The invention relates to the field of microorganisms, in particular to a plant fermented product of Saccharomyces cerevisiae and its application and an external skin agent. Background technique [0002] The free radical aging theory (Harman, D. Aging: A theory based on free radical and radiation chemistry. J. Gerontol. 1956, 11: 289-300) believes that excessive free radicals are an important cause of biological aging. According to this theory, excess active oxygen free radicals in the body interact with unsaturated fatty acids to produce malondialdehyde and other substances, and malondialdehyde interacts with proteins on cell membranes to produce brown pigment, which deposits on the skin to become various stains. Excessive free radicals can also make the collagen fibers and elastic fibers in the skin undergo cross-linking, denaturation, brittleness, and loss of elasticity. When the skin is insufficient in moisture, it is easy to break the elastic fibers, ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A61K8/99A61Q19/08C12R1/865
Inventor 方兆华祝坤章漳张佳伟李慧陈明华席淑云
Owner JALA GROUP CORPORATION
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