A method of extracting volatile oil active components from zedoray rhizome and applications of the active components

A technology of zedoary volatile oil and active components, which is applied in the field of extracting anti-liver cancer active components, can solve the problems of complicated process, high requirements for equipment and extraction conditions, and low extraction rate, and achieve simple process, large amount of preparation, and strong anti-liver cancer active effect

Pending Publication Date: 2016-06-22
TIANJIN YAOYU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The extraction methods of the anti-cancer substance zedoary oil in zedoary are mainly traditional steam distillation and supercritical extraction. This method is complex in process, requires high equipment and extraction conditions, and has a low extraction rate.

Method used

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  • A method of extracting volatile oil active components from zedoray rhizome and applications of the active components

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Use the dry tuber of Zedoary Curcuma as raw material, use ultrafine pulverizer to grind into powder at the temperature of -10°C, control the grinding time in 20 to 30 minutes, weigh 5kg, stir and extract with 20L 98% ethyl acetate for 10h, water bath Heating is constant at 65°C. The supernatant was collected by centrifugation, and then the extraction was repeated 3 times. The supernatant and the precipitate were collected respectively. The supernatant was combined and concentrated in a rotary evaporator, and transferred to a constant temperature blast drying oven at 60°C for drying to obtain a crude extract. The obtained crude extract was redissolved in n-hexane-ethanol binary mixed solvent system, and subjected to vacuum filtration to obtain an extract with a crude extract concentration of 100 mg / mL. The extract was separated by HPLC preparative chromatography, and the chromatographic column used was a normal phase silica gel axial pressurized chromatographic column (I...

Embodiment 2

[0019] Use the dry tuber of Zedoary Curcuma as raw material, use ultrafine pulverizer to grind into powder at the temperature of -10°C, control the grinding time in 20 to 30 minutes, weigh 5kg, stir and extract with 20L 98% ethyl acetate for 10h, water bath Heating is constant at 65°C. The supernatant was collected by centrifugation, and then the extraction was repeated 3 times. The supernatant and the precipitate were collected respectively. The supernatant was combined and concentrated in a rotary evaporator, and transferred to a constant temperature blast drying oven at 60°C for drying to obtain a crude extract. The obtained crude extract was redissolved in n-hexane-ethanol binary mixed solvent system, and subjected to vacuum filtration to obtain an extract with a crude extract concentration of 100 mg / mL. The extract was separated by HPLC preparative chromatography, and the chromatographic column used was a normal phase silica gel axial pressurized chromatographic column (I...

Embodiment 3

[0021] Use the dry tuber of Zedoary Curcuma as raw material, use ultrafine pulverizer to grind into powder at the temperature of -10°C, control the grinding time in 20 to 30 minutes, weigh 5kg, stir and extract with 20L 98% ethyl acetate for 10h, water bath Heating is constant at 65°C. The supernatant was collected by centrifugation, and then the extraction was repeated 3 times. The supernatant and the precipitate were collected respectively. The supernatant was combined and concentrated in a rotary evaporator, and transferred to a constant temperature blast drying oven at 60°C for drying to obtain a crude extract. The obtained crude extract was redissolved in n-hexane-ethanol binary mixed solvent system, and subjected to vacuum filtration to obtain an extract with a crude extract concentration of 100 mg / mL. The extract was separated by HPLC preparative chromatography, and the chromatographic column used was a normal phase silica gel axial pressurized chromatographic column (I...

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Abstract

A method of extracting volatile oil active components from zedoray rhizome is provided. The method includes subjecting zedoray rhizome dry tubers which are adopted as raw materials to ultrafine grinding, stirring and extracting with ethyl acetate, filtering to obtain a supernatant liquid, concentrating and drying in a rotary evaporator to obtain a crude extract product, dissolving the crude extract product into an n-hexane-ethanol binary solvent system, and separating through high performance preparative liquid chromatography by adopting a normal-phase silica gel axially-pressurized chromatographic column, wherein a binary mobile phase adopting n-hexane as an A phase and adopting methanol as a B phase is adopted, gradient elution is performed for 30 min with the concentration of the ethanol that is the B phase changing from 0 to 50%, eluate from the 15.5 min to the 17 min is collected according to an ultraviolet absorption spectrum, adopted as target components, and subjected to rotary evaporation until a product is dry, and the product is the target volatile oil active components. The method adopts the zedoray rhizome dry tubers as the raw materials, and is high in extracting efficiency, rapid and simple in preparing process and capable of popularization and large-scale production. The obtained volatile oil active components can be used for preparing anti-liver-cancer medicines.

Description

technical field [0001] The invention relates to the technical field of extracting traditional Chinese medicines, in particular to a method for extracting anti-liver cancer active components from the traditional Chinese medicine curcuma tuber. Background technique [0002] Curcuma, also known as literature, scientific name Curcumazedoary, is the rhizome of Zingiberaceae Curcuma. Curcuma has the effects of promoting qi and breaking blood, eliminating stagnation and relieving pain. Modern pharmacological studies have shown that zedoary volatile oil has anti-tumor and antibacterial effects, and curcumol has anti-tumor effects and anti-early pregnancy effects. In addition, curcuma has significant effects on cardiovascular, gastrointestinal smooth muscle, and acute renal failure, and also has hepatoprotective effect. [0003] Curcuma contains two major components, zedoary volatile oil and curcuminoids. Curcuma contains about 2% volatile oil, and the main components in the oil a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A61P35/00A61K125/00
Inventor 张耀洲
Owner TIANJIN YAOYU BIOLOGICAL TECH
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