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Amniotic membrane patch for treating skin wound and preparation method thereof

A technology of skin trauma and amnion, which is applied in the field of amnion patch for the treatment of skin trauma and its preparation, can solve the problems of cumbersome production of skin coverings, high difficulty, poor repair effect, etc., and achieve rapid repair of skin wounds, low cost, and faster The effect of repair speed

Inactive Publication Date: 2016-06-22
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a tissue-engineered skin covering that is simple to make and has a good repairing effect for the treatment of skin wounds, aiming at the defects such as cumbersome production, high difficulty and poor repairing effect in the prior art. Amnion patch and preparation method thereof

Method used

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preparation example Construction

[0022] Specifically, the preparation method of the amnion patch for treating skin wounds provided by the present invention comprises the following steps:

[0023] S1, obtaining decellularized amniotic membrane and adipose stem cells respectively;

[0024] S2. Inoculate the adipose-derived stem cells on the decellularized amnion and culture in culture until the cells completely cover the decellularized amnion.

[0025] The amniotic membrane is the thickest basement membrane in the human body. It comes from a wide range of sources. Most of it is the waste of pregnant women after giving birth. The cost is low and there is no ethical problem. The amniotic membrane is divided into five layers: epithelial layer, basement membrane, compact layer, fibroblast layer, and spongy layer; among them, the amnion basement membrane contains fibronectin, laminin, type IV collagen, heparan sulfate proteoglycan, and other Proteoglycan macromolecules and some cell growth factors such as epidermal...

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Abstract

The invention relates to a preparation method of an amniotic membrane patch for treating skin wound. The preparation method of the amniotic membrane patch for treating the skin wound comprises the following steps of respectively acquiring a denuded amniotic membrane and adipose-derived stromal cells; inoculating the adipose-derived stromal cells onto the denuded amniotic membrane, and culturing in a culture medium until the cells fully cover the denuded amniotic membrane. The differentiation potential of the adipose-derived stromal cells and the stimulation and induction functions of the denuded amniotic membrane on the adipose-derived stromal cells are utilized, so that the adipose-derived stromal cells are promoted to differentiate to form epithelial cells; the denuded amniotic membrane is lower in antigenicity, and can provide sufficient nutrient substances for the cells so as to keep the activity and the normal growth of the cells, so that the survival rate and the conversion rate of the adipose-derived stromal cells are higher, the skin repair effect is improved, and the aim of quickly repairing the skin wound is achieved.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to an amnion patch for treating skin wounds and a preparation method thereof. Background technique [0002] The skin is the barrier between the human body and the external environment. It not only plays the roles of protection, secretion, metabolism and sensation, but also participates in the immune response and maintains the stability of the internal environment of the body. As the most exposed skin of the human body, the incidence of injury and large-scale burn accidents is relatively high. Early burn wound coverage can inhibit bacterial growth, prevent infection, and prevent loss of water and electrolytes; autologous skin transplantation is the first choice for the treatment of burns, but for large area burns, autologous skin cannot meet the needs, while allogeneic skin can cause obvious immune rejection Therefore, as of now, the lack of skin source in patients with severe trau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/38A61L27/36
CPCA61L27/3834A61L27/3604A61L27/60A61L2430/40
Inventor 曾宪卓张哲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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