A method for preparing bacteria and algae cells by two rounds of co-cultivation and flocculation
A technology of co-cultivation and charcoal algae is applied in the field of preparing bacteria and algae cells by two rounds of co-cultivation and flocculation, which can solve problems such as increasing the dry weight of plants, improve growth rate and biomass, reduce production and development costs, and reduce Effects of industrial production and development application costs
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Embodiment 1
[0029] 1) The rice endophytic Pantoea strains were purchased from the China Agricultural Microorganism Collection Center (ACCC), coded as 10454. After the strains were activated by conventional methods, they were inoculated into a Erlenmeyer flask containing 10 mL of LB liquid medium, and carried out For expansion culture, the bacteria liquid in the logarithmic growth phase after 16 hours of expansion culture is inoculated according to the volume ratio of bacterial liquid: liquid LB medium = 1:10, and then the second expansion culture is carried out. The culture conditions are 37°C, 200rpm constant temperature shaker Cultivate in the dark. The second expansion culture 10h to OD 600 =1 is used as the seed cell of the co-cultivation of bacteria and algae, according to the growth curve, the bacterial growth is in the logarithmic phase, and the effective number of viable bacteria (CFU, Colony-Forming Units) in the culture solution is measured by the conventional plate counting met...
Embodiment 2
[0035] 1) The rice endophytic Pantoea strains were purchased from the China Agricultural Microorganism Collection Center (ACCC), coded as 10454. After the strains were activated by conventional methods, they were inoculated into a Erlenmeyer flask containing 10 mL of LB liquid medium, and carried out For expansion culture, the bacteria liquid in the logarithmic growth phase after 16 hours of expansion culture is inoculated according to the volume ratio of bacterial liquid: liquid LB medium = 1:10, and then the second expansion culture is carried out. The culture conditions are 37°C, 200rpm constant temperature shaker Cultivate in the dark. The second expansion culture 10h to OD 600 =1 is used as the seed cell of the co-cultivation of bacteria and algae, according to the growth curve, the bacterial growth is in the logarithmic phase, and the effective number of viable bacteria (CFU, Colony-Forming Units) in the culture solution is measured by the conventional plate counting met...
Embodiment 3
[0041] 1) The rice endophytic Pantoea strains were purchased from the China Agricultural Microorganism Collection Center (ACCC), coded as 10454. After the strains were activated by conventional methods, they were inoculated into a Erlenmeyer flask containing 10 mL of LB liquid medium, and carried out For expansion culture, the bacteria liquid in the logarithmic growth phase after 16 hours of expansion culture is inoculated according to the volume ratio of bacterial liquid: liquid LB medium = 1:10, and then the second expansion culture is carried out. The culture conditions are 37°C, 200rpm constant temperature shaker Cultivate in the dark. Continuous expansion was carried out three times, and the culture conditions were 37°C, 200rpm constant temperature shaker and dark culture. The third expansion culture 10h to OD 600 =1 is used as the seed cell of the co-cultivation of bacteria and algae, according to the growth curve, the bacterial growth is in the logarithmic phase, and t...
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