The invention discloses a method for preparing bacterium-
algae cells through a two-wheel coculturing and
flocculation method. The method comprises the steps of 1, conducting the first cycle of coculturing on rice endogenous
pantoea which is added for the first time and
chlorella under illumination, so that after culturing is ended, a bacterium-
algae coculturing solution of the first cycle is obtained; 2, supplementing rice endogenous
pantoea which is added for the second time into the bacterium-
algae coculturing solution of the first cycle, conducting the second cycle of bacterium-algae coculturing under illumination, so that after culturing is ended, a bacterium-algae coculturing solution of the second cycle is obtained; 3, conducting
flocculation collection treatment on the bacterium-algae coculturing solution of the second cycle, so that the bacterium-algae cells are obtained. According to the method, bacterium-algae coculturing is conducted through the rice endogenous
pantoea and the
chlorella, the growth rate of the algae cells is raised,
biomass of the algae cells is increased, meanwhile,
flocculation performance of the algae cells is improved, application of chemical flocculating agents and toxic harm to the algae cells are reduced, algae
cell collection efficiency is improved, algae
cell collection cost is lowered, and thereby industrial production and development and application cost of
chlorella are remarkably lowered.