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Extraction method for oxalis corymbosa flavonoid compound

A technology of sorrel flavonoids and safflower sorrel, which can be used in pharmaceutical formulas, plant raw materials, medical preparations containing active ingredients, etc., can solve the problems of few reports on the extraction conditions of flavonoids, and achieve greater utilization Range, Yield Enhancement and Quality Effects

Inactive Publication Date: 2016-06-29
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that flavonoids extracted from natural plants can effectively scavenge a variety of free radicals, and have biological activities such as anti-oxidation, anti-tumor, anti-inflammation, immune regulation, and anti-virus. Very important application value and research significance, but there are few reports on the extraction conditions of safflower flavonoids

Method used

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  • Extraction method for oxalis corymbosa flavonoid compound
  • Extraction method for oxalis corymbosa flavonoid compound
  • Extraction method for oxalis corymbosa flavonoid compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Determination of the content of flavonoids

[0023] First formulate the standard curve of rutin: take 0.02g of the standard rutin, place it in a 100mL volumetric flask, dissolve it with 70% (v / v) ethanol and dilute to the mark. Accurately pipet 0mL, 0.2mL, 0.4mL, 0.8mL, 1.2mL, 1.6mL, 2.0mL of rutin standard solution into 10mL graduated test tube with stopper, fill each tube with pure water to 2.0mL, add 5% (w / v) Sodium nitrite 0.3mL, shake well and place for 6min, add 10% (w / v) aluminum trichloride 0.3mL, shake well and place for 6min, add 4% (w / v) sodium hydroxide 4mL, and finally use Constant volume of pure water, stand for 10min, measure its absorbance at 510nm, and establish a regression equation: A (absorbance) = KC (rutin concentration) + b. According to this method, a standard curve of rutin was obtained.

[0024] Determination method of sample flavonoids: take 0.1mL sample extract in a 10mL test tube, make up with pure water to 2.0mL, add 0.3mL of 5% s...

Embodiment 2

[0025] Example 2: The effect of the type of extractant on the extraction of flavonoids

[0026] The extraction temperature was 60℃, the extraction time was 2.0h, and the material-liquid ratio was 1:50. The effects of methanol, ethanol, water, n-butanol and ethyl acetate as extractants on the extraction of flavonoids were investigated. The results show that different extraction solvents have a greater impact on the extraction of flavonoids from shamrock. Ethyl acetate as an extractant has a poor extraction effect of flavonoids, which is significantly lower than that of other solvents. The extraction effect of methanol is the best, but methanol is toxic and costly. The extraction effect of ethanol is better, non-toxic, safe and environmentally friendly. Therefore, from the perspective of extraction effect and safety, ethanol is the best reagent for extracting flavonoids from shamrock. (see figure 2 )

Embodiment 3

[0027] Example 3: Effect of ethanol concentration on the extraction of flavonoids

[0028] Based on the selection of the best extraction solvent, the influence of the reagent at different concentrations (40%, 50%, 60%, 70%, 80%) on the content of flavonoids was studied, and the appropriate concentration was selected. The results showed that as the ethanol concentration increased, the extracted content of flavonoids increased, reaching the maximum when the ethanol concentration was 70%, but when the ethanol concentration exceeded 70%, the extracted flavonoids decreased, indicating that safflower pulp The extraction of flavonoids is preferably 70% ethanol. (see image 3 )

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Abstract

The invention discloses an extraction method for an oxalis corymbosa flavonoid compound. The method comprises the following steps that oxalis corymbosa is selected and ground into powder, and the oxalis corymbosa powder is extracted by adding an ethanol solution to obtain an extracting solution; precipitates in the extracting solution are removed through centrifugation by adopting a high-speed centrifuge to obtain supernatant liquor; the supernatant liquor is concentrated on a rotary evaporator to remove solvent and then placed in a vacuum freeze dryer for freeze drying, and then the oxalis corymbosa flavonoid extract is obtained. By studying the extraction method and technology for the oxalis corymbosa flavonoid compound, a reference is supplied to further study on the antioxidant and medicinal effects of the oxalis corymbosa flavonoid compound, and the using range of the oxalis corymbosa is widened. The technology is simple and easy to operate and has the better reference value.

Description

technical field [0001] The invention relates to a method for extracting flavonoids from safflower sorrel. Background technique [0002] Sorrel has many varieties such as red-flowered sorrel and purple-leaved sorrel, and most of them are perennial herbaceous plants of the family Oxalis. Sorrel is widely distributed all over the world. In China, it is mainly distributed in North China, Central China, South China, Jiangxi, Sichuan and Yunnan. It is an excellent ground cover and potted plant. The whole herb of sorrel can be used as medicine. It is cold in nature and sour in taste, and it returns to the liver and small intestine meridian. Clinically, it is mainly used to treat various diseases such as pneumonia, tonsillitis, acute hepatitis and mumps. Western Hubei Tujia is often used to treat bruises and bruises, sore throat, expectorant and asthma, dysentery, jaundice, urinary tract infection, stones, liver fever and convulsions in children, etc.; external use to treat bruise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 廖鲜艳王亚茜许慧敏陈楠楠黄俊逸
Owner SHANGHAI UNIV
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