Preparation method of edible and medicinal fungi protein peptide-zinc chelate
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A technology of zinc chelate and edible and medicinal fungi, which is applied in food science, protein food processing, protein food ingredients, etc., can solve the problems of complex synthesis, low yield, and low zinc content, and achieve high extraction rate and low price. Low, easily absorbed effect
Inactive Publication Date: 2016-07-20
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology
The current zinc supplementation agents are complex in synthesis, low in yield, low in zinc content, accompanied by adverse reactions such as mild nausea, vomiting, constipation, etc., and are not suitable for patients with diabetes, anemia, and prostatic hypertrophy
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Embodiment 1
[0028] A preparation method of edible and medicinal mycoprotein peptide-zinc chelate, the specific steps are as follows:
[0029] Step 1: Ultrafinely crush Grifola frondosa, mix the crushed Grifola frondosa with distilled water at a ratio of 1g:20mL, adjust the pH value to 9.0 with 2wt% NaOH solution, extract by ultrasonic for 10min, extract in a water bath for 60min, and temperature 60 ℃, filter with gauze after extraction, the filtrate is the alkali-extracted grifola frondosa protein solution, adjust the pH of the alkali-extracted grifola frondosa protein solution to 3.0 with hydrochloric acid, carry out protein precipitation, centrifuge at 4000r / min for 5min to obtain the precipitated protein, wash with water to medium centrifuged again, and the precipitate was freeze-dried to obtain the grifola protein, with an extraction rate of 79.61%;
[0030] Step 2: Dissolve the grifola frondosa protein in water so that the protein concentration reaches 3.0%, adjust the pH of the grif...
Embodiment 2
[0038] A preparation method of edible and medicinal mycoprotein peptide-zinc chelate, the specific steps are as follows:
[0039] Step 1: Superfine the Pleurotus eryngii, mix the crushed Pleurotus eryngii with distilled water at a ratio of 1g:60mL, adjust the pH to 11.0 with 1wt% NaOH, ultrasonically extract for 50min, and extract in a water bath for 120min at a temperature of 100°C. After extraction, filter with gauze, the filtrate is alkali-extracted Pleurotus eryngii protein solution, adjust the pH of the alkaline-extracted Pleurotus eryngii protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge to obtain precipitated protein, wash with water until neutral and centrifuge again, and freeze the precipitate Pleurotus eryngii protein can be obtained by drying, and its extraction rate reaches 83.74%;
[0040] Step 2: Dissolve Pleurotus eryngii protein in water so that the protein concentration reaches 3.0%, adjust the pH to 7.0, keep the temperature a...
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Abstract
The invention relates to a preparation method of edible and medicinal fungi protein peptide-zinc chelate. The preparation method comprises the following steps: extracting proteins from edible and medicinal fungus by an alkali-soluble acid precipitation method or an ammonium sulfate precipitation method; performing restricted enzymatic hydrolysis on the edible and medicinal fungi proteins by alkaline proteases, neutral proteases or compound proteases, so that an enzymatic hydrolysate of the edible and medicinal fungi proteins is prepared; and chelating zinc in inorganic zinc acetate or zinc sulfate with the edible and medicinal fungi protein peptide, so that the edible and medicinal fungi protein peptide-zinc chelate is prepared. The alkali-soluble acid precipitation method or the ammonium sulfate precipitation method utilized in the method is capable of obviously improving extraction rates of the edible fungi proteins; and the time of the enzymatic hydrolysis is controlled so as to effectively control degradation degrees of the proteins. A preparation technology of the polypeptide-zinc chelate is simple. The zinc-peptide chelate prepared by the preparation metahod is unique in chelate system and transport mechanism, and is easy to absorb, safe, non-toxic and low in cost; the zinc-peptide chelate is capable of supplementing amino acids and zinc at the same time, so that the zinc-peptide chelate will become the first choice among zinc supplements. The preparation method of edible and medicinal fungi protein peptide-zinc chelate provides a new idea to edible fungi applications.
Description
technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a preparation method of edible mushroom protein peptide-zinc chelate. Background technique [0002] Zinc is an essential trace element for the human body. It is a component of various enzymes or an activator of enzymes. It is important for the growth of the body. [0003] It plays an important role in many aspects such as growth, tissue regeneration, appetite promotion, normal development of sexual organs and sexual function, protection of skin health, and enhancement of immune function. Although zinc sources are very abundant in nature, the absorption and metabolism of zinc in the human body is limited by various factors. Zinc deficiency has become a public health problem in my country and many developing countries. The zinc supplements approved by the Ministry of Health include inorganic zinc and organic zinc. Early inorganic zinc salts include zinc sulfate, zinc...
Claims
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