an oligonucleotide probe

Abstract

The invention belongs to the technical field of nucleic acid probes and provides an oligonucleotide probe. The probe consists of a sequence complementary to the target sequence, a G-quadruplex blocking sequence and a G-quadruplex sequence from the 5'-3' end. The G-quadruplex blocking sequence is complementary to part of the G-quadruplex sequence, forming a stable hairpin structure, and enclosing the G-quadruplex sequence inside the probe. When detecting target molecules, the probe first recognizes the target sequence and forms a double-stranded structure, then under the digestion of Lambda exonuclease, the hairpin structure is opened, the G-quadruplex sequence is released, and the cycle repeats, and finally the trace target Molecular information is converted into a large number of G-quadruplex sequences, and then converted into readable optical, electrical and other signals through the G-quadruplex sequences. The probe has the advantages of good stability, low cost, easy preparation, high throughput, high sensitivity and specificity, can realize the direct detection of single-stranded nucleic acid, and can also detect double-stranded nucleic acid and other molecules for indirect detection.

Description

technical field

[0001] The invention relates to nucleic acid probe technology, in particular to an oligonucleotide probe capable of detecting target molecules. Background technique

[0002] Oligonucleotide is a kind of short-chain nucleic acid with only dozens of bases. Because of its inherent nucleic acid complementary hybridization characteristics and simple and operable structural characteristics, it is often used as a nucleic acid after being labeled with a certain chemical group. Probes, which convert nucleic acid signals into optically or electrically readable signals, are powerful tools for nucleic acid detection and analysis. Such probes are oligonucleotide probes. The probe labeling method used earlier is mainly radioactive isotope labeling, but it is easy to cause radioactive contamination, and there are problems such as short half-life of the isotope, instability, high cost, inconvenient operation, and difficulty in commercialization. Therefore, in recent years, ...

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