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A method for improving the efficiency of enzymatic hydrolysis and saccharification of lignocellulosic raw materials

A technology for lignocellulosic raw material and saccharification efficiency, which is applied in the field of enzyme engineering and can solve the problems of increasing additional costs and the like

Active Publication Date: 2020-07-07
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adding additives in the enzymatic hydrolysis process will undoubtedly increase additional costs, so additives with low cost, abundant sources, easy access, easy preparation, and high efficiency have more application prospects

Method used

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  • A method for improving the efficiency of enzymatic hydrolysis and saccharification of lignocellulosic raw materials
  • A method for improving the efficiency of enzymatic hydrolysis and saccharification of lignocellulosic raw materials
  • A method for improving the efficiency of enzymatic hydrolysis and saccharification of lignocellulosic raw materials

Examples

Experimental program
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Effect test

Embodiment 1

[0036] (1) Take a certain amount of hardwood and process it into wood chips with a length of about 1.5cm, a width of about 2.5cm, and a thickness of about 0.2cm, and balance the moisture after air-drying.

[0037] (2) Green liquor pretreatment of hardwood. In a self-made 10×1L oil bath, weigh 100g air-dried wood chips equivalent to absolute dryness in each tank, add the cooking liquid with a solid-to-liquid ratio of 1:4 (g / mL), use 20% of alkali, and have a sulfide degree of 25%, wood chips and cooking liquid are evenly mixed, pre-soaked at 80°C for 30 minutes, then heated to 160°C at a rate of 2°C / min, and the pretreatment is completed after 60 minutes of heat preservation. After the cooking tank is taken out and cooled to room temperature with cold water, the pretreated wood chips are rinsed with hot water to wash away the residual chemicals and lignin in the wood chips and dehydrated by centrifugal dehydration. The moisture is balanced in a sealed container and the yield is...

Embodiment 2

[0044] (1) Take a certain amount of hardwood and process it into wood chips with a length of about 1.5cm, a width of about 2.5cm, and a thickness of about 0.2cm, and balance the moisture after air-drying.

[0045] (2) Pretreatment of hardwood with acidic sodium bisulfite. In a self-made 10×1L oil bath, each tank weighed air-dried wood chips equivalent to 100g of dry wood, and added the cooking liquid with a solid-liquid ratio of 1:5 (g / mL), and the amount of sodium bisulfite (relative to the dry raw material ) is 6%, after the wood chips and the cooking liquid are evenly mixed, after pre-soaking at 80°C for 30 minutes, the temperature is raised to 170°C at a rate of 2°C / min, and after holding for 30 minutes, the pretreatment is completed. Take out the cooking tank and cool it to room temperature with cold water, rinse the pretreated wood chips with hot water overnight, wash away the residual chemicals and lignin in the wood chips, and centrifugally dehydrate, balance the water...

Embodiment 3

[0052] (1) Take a certain amount of hardwood and process it into wood chips with a length of about 1.5cm, a width of about 2.5cm, and a thickness of about 0.2cm, and balance the moisture after air-drying.

[0053] (2) Perform sodium sulfite-formaldehyde pretreatment on hardwood. In a self-made 10×1L oil bath, weigh 100g of air-dried wood chips equivalent to dryness in each tank, add the cooking liquid with a solid-liquid ratio of 1:4 (g / mL), and use 12% sodium sulfite (relative to dryness raw materials). %, the amount of formaldehyde is 2.9%, after the wood chips and the cooking liquid are mixed evenly, after pre-soaking at 80°C for 30 minutes, the temperature is raised to 170°C at a rate of 2°C / min, and after holding for 60 minutes, the pretreatment is completed. Take out the cooking tank and cool it to room temperature with cold water, rinse the pretreated wood chips with hot water overnight, wash away the residual chemicals and lignin in the wood chips, and centrifugally de...

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Abstract

The invention relates to a method for improving wood fiber material enzyme hydrolysis saccharification efficiency and belongs to the technical field of enzyme engineering.The method is simple in technology, low in cost and capable of efficiently improving wood fiber material enzyme hydrolysis saccharification efficiency.The method comprises the steps that after sulfate pulping waste liquid is subjected to floating oil recovery and acid precipitation, the solution pH is adjusted through alkali to be 11, centrifugal separation is performed to remove lower-layer sediment and other alkaline undissolved matter, the pH of liquid supernatant is adjusted through 2 mol / L sulfuric acid to be 5, centrifugal separation is performed, precipitate is abandoned, the pH of the liquid supernatant continues to be adjusted through the2 mol / L sulfuric acid to be 2, centrifugal separation is performed, the liquid supernatant is abandoned, the precipitate is washed with sulfuric acid with the pH being 2, sulfate xylogen is obtained, the sulfate xylogen is added into an acetic acid-sodium acetate buffer solution to be stirred to be uniform and used as an auxiliary agent, the auxiliary agent is added into a pretreated enzymolysis substance wood fiber raw material to be mixed to be uniform, an enzyme solution is added for an enzyme hydrolysis saccharification reaction, and upper-layer sugary clear liquid is centrifugally collected after the reaction is finished.

Description

technical field [0001] The invention belongs to the technical field of enzyme engineering, and mainly relates to a method for improving the enzymatic hydrolysis and saccharification efficiency of lignocellulosic raw materials. Background technique [0002] The depletion of fossil energy is seriously restricting the development and progress of world civilization, and the search for alternative renewable resources has become the research focus of countries all over the world. Since the 1970s, bioenergy, as a renewable potential energy source, has been favored by researchers from all over the world. At the same time, the application of bioenergy can also reduce the greenhouse gas CO produced by the use of traditional fossil energy. 2 And a small amount of harmful gases containing sulfur and nitrogen, which can effectively alleviate the greenhouse effect. As a second-generation biofuel, lignocellulosic raw materials have the characteristics of large output and low cost, which ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02C12P7/10
CPCC12P7/10C12P19/02C12P19/14C12P2203/00Y02E50/10
Inventor 金永灿王琰朱杨苏杨益琴
Owner NANJING FORESTRY UNIV