Umbilical cord blood stem cell injection solution with anti-aging effect and preparation method thereof

An umbilical cord blood stem cell and anti-aging technology, applied in the field of cell biology, can solve problems such as inability to prevent organ aging, and achieve the effects of improving human immunity, improving blood circulation, and strong activity.

Inactive Publication Date: 2016-08-03
SHANGHAI HUAYAN MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the most commonly used anti-aging methods are only for anti-aging skin care, such as sheep placenta injection, botulinum toxin injection, use of hormones, use of skin care essence, etc., are only local effects, not systemic, and cannot prevent and control organ aging

Method used

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  • Umbilical cord blood stem cell injection solution with anti-aging effect and preparation method thereof
  • Umbilical cord blood stem cell injection solution with anti-aging effect and preparation method thereof
  • Umbilical cord blood stem cell injection solution with anti-aging effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of an umbilical cord blood stem cell injection with anti-aging effect.

[0048] Specifically include the following steps:

[0049] Step 1. Umbilical cord blood screening and storage: screening and collection of umbilical cords from healthy full-term maternal volunteers, placing them in umbilical cord blood storage bags at 4°C, and separating them within 24 hours;

[0050] Step 2. Separation of umbilical cord blood: the umbilical cord blood is removed by two-step centrifugation to remove most of the red blood cells, and the mononuclear cell layer we want is separated. It is cracked twice with high-temperature sterilized ultrapure water to remove the separated red blood cells. And keep the separated plasma.

[0051] Step 3, umbilical cord blood nucleated cryopreservation: collect cells and count and viability (cytometer count), cell count results are shown in Table 1, according to 3 × 10 7 / ml / tube for cryopreservation, using 90% plasma and 10% m...

Embodiment 2

[0063] Example 2 Preparation of a final product of umbilical cord blood stem cell injection with anti-aging effect

[0064] Step 1. Resuscitate the qualified cord blood stem cells in a 37°C water bath for 2 minutes.

[0065] Step 2: Wash twice with clinical injection grade saline,

[0066] Step 3, dissolving 2wt% of human serum albumin and 10-20v% of umbilical cord blood autologous plasma in the compound electrolyte solution,

[0067] Step 4: Cell count and viability were kept at 95%, and the cells were counted according to 5×10 5 ~10×10 5 / ml resuspended in the complex solution of step 3.

[0068] The umbilical cord blood stem cell injection prepared by this method contains the following quantities or concentrations of components:

[0069] Cord blood stem cells: 5×10 5 ~10×10 5 / ml;

[0070] Human albumin: 2wt%;

[0071] Cord blood autologous plasma: 10-20v%;

[0072] Compound electrolyte solution: 78-88v%.

Embodiment 3

[0073] Example 3 : This embodiment relates to the animal experiment of umbilical cord mesenchymal stem cell injection of the present invention infused to aging model rats:

[0074] Determination of anti-aging indicators includes: 1. Appearance characteristics: body weight, coat color, action, and activity; 2. Biochemical indicators: activity of superoxide dismutase (SOD), content of malondialdehyde (MDA), thymus and spleen IL-2, IL-10 content, liver and kidney ratio. Specific steps are as follows:

[0075] Step 1, establishment of aging model and group comparison experiment

[0076] Take 24 SD rats aged 6-8 weeks, randomly divide 8 into the normal group, and establish the aging model of the remaining 16 rats. 8 rats in the normal group were subcutaneously injected with normal saline, once a day, for 8 consecutive weeks; the other 16 rats were injected with D-galactose 120 mg / kg subcutaneously, once a day, for 8 consecutive weeks.

[0077] From the 9th week, the normal gro...

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Abstract

The invention discloses an umbilical cord blood stem cell injection solution with an anti-aging effect and a preparation method thereof. The umbilical cord blood stem cell injection solution contains the following components by volume or concentration: 5X10<5> to 10X10<5> umbilical cord blood stem cells per ml, 2wt% of human albumin, 10-20v% of umbilical cord blood autologous plasma and 78-88v% of a compound electrolyte solution. According to the umbilical cord blood stem cell injection solution, the used stem cells are blood related stem cells and have the characteristics of amplification and directional differentiation; the cells return to an injured part when receiving an injured part signal; the cells are subjected to amplification and induced differentiation at the injured part and the stem cells of the injured part are promoted to recover functions, thereby quickly repairing and replacing injured cells and tissues; and cell factors, growth factors and tissue related factors are secreted quickly in time, thereby improving blood circulation of a human body, metabolic function, tissue repair capability and immunity, and further achieving the purpose of aging resistance.

Description

technical field [0001] The invention relates to an umbilical cord blood stem cell injection with anti-aging effect and a preparation method thereof, belonging to the technical field of cell biology. Background technique [0002] Stem cells are primitive cells with self-replication and multi-directional differentiation potential, and are the origin cells that form various tissues and organs of living organisms. Stem cell technology is one of the most important technologies in the field of life science and technology. It can derive cells into various types of cells and their tissues, resulting in some new therapeutic technologies, such as stem cell transplantation, tissue and organ reconstruction, and gene therapy. People can use their own or others' healthy stem cells and their derived tissues and organs to replace diseased or aging tissues and organs. [0003] Stem cells can be divided into embryonic stem cells (Embryonic stem cells) and adult stem cells (Adult stem cells) ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/51A61K9/08A61P39/06A61P37/04
CPCA61K35/51A61K9/0019A61K9/08
Inventor 朱兵锐
Owner SHANGHAI HUAYAN MEDICINE TECH CO LTD
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