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Self-flocculating fermentation production of alpha-arbutin

A technology of self-flocculation and arbutin, applied in the field of microbial engineering, can solve the problems of long time consumption and high production cost, and achieve the effect of reducing production cost and shortening production time

Inactive Publication Date: 2016-08-03
陈礼明
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Problems solved by technology

[0004] At present, suspension fermentation is mostly used for the biological synthesis of α-arbutin in China, and the fermentation broth needs to be centrifuged in the later stage of production, so the production process takes a long time and the production cost is also high

Method used

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  • Self-flocculating fermentation production of alpha-arbutin

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specific Embodiment approach

[0024] detailed description Below in conjunction with embodiment the present invention is described in further detail, but will point out here, the present invention is not limited to the scope of embodiment.

Embodiment

[0026] A method for producing α-arbutin by self-flocculation fermentation, comprising the following steps:

[0027] (1) Preparation of α-arbutin:

[0028] ①Slant seed culture: configure slant medium (5% sucrose, 1% peptone, 0.2% yeast powder, 0.05% magnesium sulfate, 0.2% dipotassium hydrogen phosphate, 0.2% sodium chloride, 2% agar, pH7.0, high pressure Sterilization), Xanthomonas campestris ( Xanthomonas campestris) Inoculate on slant medium and incubate at 35°C for 36 hours.

[0029] ②Seed liquid culture: Pick the strains with an inoculation loop, inoculate them in a 250ml Erlenmeyer shaker flask with 50ml of basic fermentation broth, shake on a shaker at 35°C at 160 rpm for 30 hours. Basic fermentation broth: 5% sucrose, 1% peptone, 0.3% beef extract, 0.1% yeast extract, 0.05% magnesium sulfate, 0.2% calcium carbonate.

[0030] ③Add the 8% seed culture solution into a 500ml Erlenmeyer shaker flask filled with 200ml of basic fermentation solution, and vibrate at 180 rpm...

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Abstract

The invention belongs to the field of microbial engineering technology and relates to a novel fermentation method, a self-flocculating method, for preparing alpha-arbutin. According to the invention, xanthomonas campestris is used for fermentation production of alpha-arbutin. When a strain is inoculated into a shake flask for cultivation, millimeter-scale particles can be formed by self-flocculating within 4-8 h, and particle morphology growth is maintained during the follow-up scale-up culture. Immobilization of the strain in a fermentation broth is realized, and after-treatment process is reduced. Centrifugal separation of thalline is not required, and production cost of alpha-arbutin can be reduced. By the method, yield of alpha-arbutin is high, and the product can inhibit generation and deposition of melanin, remove color spot and effectively inhibit tyrosinase activity at low concentration.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and relates to a novel fermentation method for preparing α-arbutin: self-flocculation method. Background technique [0002] Arbutin (Arbutin) was first derived from natural green plants, from the leaves of the Vaccinium plant Bearberry, and is also found in pears, wheat and other plants. It can quickly penetrate into the skin, effectively inhibit the activity of tyrosinase in the skin, block the synthesis of dopa and dopaquinone, thereby curbing the growth of melanin, reducing skin pigmentation, removing spots and freckles, It does not produce side effects such as toxicity, irritation, and sensitization to melanocytes, so it is used in cosmetics and skin care products. In addition, arbutin also has diuretic, emollient and bactericidal functions, and is also used in urinary tract disinfectants, burn and scald drugs, and intestinal anti-inflammatory drugs. [0003] According to its ...

Claims

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Application Information

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IPC IPC(8): C12P19/44C12R1/64
Inventor 陈礼明王璐
Owner 陈礼明
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