Preparation method of edible and medicinal fungus protein peptide-ferrous chelate
A technology of edible and medicinal fungi and mycoprotein, which is applied in food science and other fields, can solve the problems of low utilization rate, high toxicity and side effects, etc., and achieve the effects of low price, easy absorption and high extraction rate
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Embodiment 1
[0026] A preparation method of Pleurotus eryngii protein peptide-ferrous chelate, the specific steps are:
[0027] Step 1: Ultrafinely pulverize Pleurotus eryngii, mix the pulverized Pleurotus eryngii with distilled water at a ratio of 1 g:20mL, adjust the pH to 9.0 with 2wt% NaOH, ultrasonically extract for 10 min, and extract in a water bath for 60 min. 60 ℃, filter with gauze after extraction, the filtrate is the alkali-extracted Pleurotus eryngii protein solution, adjust the pH of the alkaline-extracted Pleurotus eryngii protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge at 4000 r / min for 5 min to obtain the precipitated protein, wash with water Centrifuge again until neutral, and freeze-dry the precipitate to obtain Pleurotus eryngii protein, with an extraction rate of 74.16%;
[0028] Step 2: Dissolve Pleurotus eryngii protein in water so that the protein concentration reaches 3.0%, adjust the pH of the Pleurotus eryngii protein solution...
Embodiment 2
[0046] A kind of preparation method of straw mushroom protein peptide-ferrous chelate, concrete steps are:
[0047] Step 1: Ultrafinely crush the straw mushrooms, mix the crushed straw mushrooms with distilled water at a ratio of 1 g:60 mL, adjust the pH to 11.0 with 1 wt% NaOH, extract by ultrasonic for 50 min, and extract in a water bath for 120 min at a temperature of 100 ℃, filter with gauze after extraction, the filtrate is the alkali-extracted straw mushroom protein solution, adjust the pH of the alkali-extracted straw mushroom protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge to obtain the precipitated protein, wash with water until neutral and centrifuge again, and freeze the precipitate The edible and medicinal mycoprotein can be obtained by drying, and the extraction rate reaches 83.72%;
[0048] Step 2: Dissolve the straw mushroom protein in water so that the protein concentration reaches 3.0%, adjust the pH to 11.0, keep the tempe...
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