Preparation method of edible and medicinal fungus protein peptide-ferrous chelate
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A technology of edible and medicinal fungi and mycoprotein, which is applied in food science and other fields, can solve the problems of low utilization rate, high toxicity and side effects, etc., and achieve the effects of low price, easy absorption and high extraction rate
Inactive Publication Date: 2016-08-17
FUJIAN AGRI & FORESTRY UNIV
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Common iron supplements include ferrous sulfate, ferrous chloride, ferrous gluconate, ferrous lactate, etc. Although these iron supplements have high iron content and good iron supplementation effect, their utilization rate in the body is low , high toxicity and side effects, and has a special smell of metal rust
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Embodiment 1
[0026] A preparation method of Pleurotus eryngii protein peptide-ferrous chelate, the specific steps are:
[0027] Step 1: Ultrafinely pulverize Pleurotus eryngii, mix the pulverized Pleurotus eryngii with distilled water at a ratio of 1 g:20mL, adjust the pH to 9.0 with 2wt% NaOH, ultrasonically extract for 10 min, and extract in a water bath for 60 min. 60 ℃, filter with gauze after extraction, the filtrate is the alkali-extracted Pleurotus eryngii protein solution, adjust the pH of the alkaline-extracted Pleurotus eryngii protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge at 4000 r / min for 5 min to obtain the precipitated protein, wash with water Centrifuge again until neutral, and freeze-dry the precipitate to obtain Pleurotus eryngii protein, with an extraction rate of 74.16%;
[0028] Step 2: Dissolve Pleurotus eryngii protein in water so that the protein concentration reaches 3.0%, adjust the pH of the Pleurotus eryngii protein solution...
Embodiment 2
[0046] A kind of preparation method of straw mushroom protein peptide-ferrous chelate, concrete steps are:
[0047] Step 1: Ultrafinely crush the straw mushrooms, mix the crushed straw mushrooms with distilled water at a ratio of 1 g:60 mL, adjust the pH to 11.0 with 1 wt% NaOH, extract by ultrasonic for 50 min, and extract in a water bath for 120 min at a temperature of 100 ℃, filter with gauze after extraction, the filtrate is the alkali-extracted straw mushroom protein solution, adjust the pH of the alkali-extracted straw mushroom protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge to obtain the precipitated protein, wash with water until neutral and centrifuge again, and freeze the precipitate The edible and medicinal mycoprotein can be obtained by drying, and the extraction rate reaches 83.72%;
[0048] Step 2: Dissolve the straw mushroom protein in water so that the protein concentration reaches 3.0%, adjust the pH to 11.0, keep the tempe...
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Abstract
The invention belongs to the field of food biotechnology, and specifically relates to a preparation method of edible and medicinal fungus protein peptide-ferrous chelate, comprising: extracting protein from edible and medicinal fungi by alkali-dissolving acid precipitation method or ammonium sulfate precipitation method; Proteases, neutral proteases or complex proteases restrict the enzymolysis of edible and medicinal fungal proteins, and then inactivate the enzymes to prepare edible and medicinal fungal protein enzymatic hydrolysates; use inorganic ferrous chloride or ferrous sulfate in ferrous and Edible and medicinal mycoprotein peptide chelation to obtain edible and medicinal mycoprotein-ferrous chelate. The invention can significantly improve the extraction rate of edible and medicinal mycoprotein by alkali-soluble acid precipitation method or ammonium sulfate precipitation method; the degradation degree of protein can be effectively controlled by controlling the enzymatic hydrolysis time; the preparation process of the whole polypeptide-ferrous chelate is simple. The ferrous-peptide chelate prepared by the present invention has a unique chelation system and transport mechanism, is easily absorbed, safe and non-toxic, low in price, can supplement amino acids and ferrous at the same time, and will definitely become the first choice for ferrous supplements; The invention provides a new idea for the application of edible and medicinal fungi.
Description
technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a preparation method of an edible and medicinal fungus protein peptide-ferrous chelate. Background technique [0002] Ferrous iron is one of the essential trace elements for the human body. It is an essential component of hemoglobin, plays a role in transporting oxygen in the human body, and also participates in many important physiological processes such as cell proliferation and differentiation, energy metabolism, and detoxification. According to the report of the World Health Organization, iron deficiency exists in 30% of the world's population, and iron deficiency anemia is the most common nutritional disease in the world. More than 95% of nutritional anemia patients in my country are iron-deficiency anemia; the prevalence of iron-deficiency anemia in children under 7 years old is about 20%-70%. At present, the prevention and treatment of iron deficiency anemia ...
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