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Method for extracting functional sweetener from malus plant

A technology of sweetener and Malus, which is applied in the direction of food extraction, food ingredients containing natural extracts, food ingredients as taste improvers, etc. It can solve the problems of small distribution area, increased cost, and less planting amount of plants of the genus Licorice , to achieve the effect of low cost, good compatibility and large output

Active Publication Date: 2016-08-31
NORTHWEST A & F UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main way to obtain trilobatin is to use the Fagaceae Shike plants as materials for extraction, such as Duosuke (alias sweet tea), but the distribution area of ​​Shike plants is small and the planting amount is small, so it is used to extract trilobites Glycosides increase cost and limit yield

Method used

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  • Method for extracting functional sweetener from malus plant
  • Method for extracting functional sweetener from malus plant

Examples

Experimental program
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Effect test

Embodiment 1

[0019] After the treated fruit or fruit residue of Malus is dried by heating and / or freezing and reducing pressure, it is soaked in an aqueous alcohol solution, heated and / or pressurized, and moderately stirred at the same time to increase the leaching rate. After the leaching is completed, the liquid part is concentrated by heating and / or reduced pressure, and then chromatographed with a macroporous resin, eluted with an aqueous alcohol solution, and the components containing the target compound are collected, and concentrated again using a high-speed countercurrent chromatograph. For fine separation, the configured solvent system is injected into the instrument in sequence, and the flow rate is controlled at a certain speed, and the components containing the target compound are collected, fully dried to obtain the sweetener composition, and the sweetener composition is detected by high performance liquid chromatography species trilobatin content.

Embodiment 2

[0021] After the stem or leaf of the treated Malus plant is dried by heating and / or freezing and reducing pressure, it is soaked in an aqueous alcohol solution, heated and / or pressurized, and moderately stirred at the same time to increase the leaching rate. After the leaching is completed, the liquid part is concentrated by heating and / or reduced pressure, and then a chromatographic column packed with polyamide with a certain particle size is used for preliminary separation, and an aqueous alcohol solution is used for elution, and the components containing the target compound are collected and concentrated. Afterwards, gel column chromatography is used for fine separation to obtain a solution containing the sweetener composition, which is dried and crystallized to obtain the sweetener composition, and the content of trilobatin in the sweetener composition is detected by high performance liquid chromatography.

[0022] Non-limiting examples of treatments for Malus plants suitab...

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Abstract

The invention discloses a functional sweetener and an extraction method thereof. The functional sweetener should contain a trilobatin component and is extracted from a malus plant. The extraction method comprises the following six main steps: digestion; extraction; preliminary separation; fine separation; concentration; and drying and crystallization. The eventually extracted functional sweetener is great in output and controllable in cost.

Description

technical field [0001] The invention belongs to the technical field of extracting and separating active components in plants, and in particular relates to a method for extracting and separating functional sweeteners from plants of the genus Malus. Background technique [0002] A sweetener is a substance that can impart sweetness to food or soft drinks. Non-sugar sweeteners have received widespread attention because they do not cause health problems such as blood sugar increases and fat accumulation. [0003] As a non-sugar sweetener, trelobatin belongs to the glycosylated derivatives of phloretin. It is generally believed that the glycosylated derivatives of phloretin are a kind of sodium / glucose cotransporter (sodium / glucose cotransporter Inhibitors of SGLT), this type of transport factor is distributed in the small intestine, kidney and other organs of the human body, and the purpose of lowering blood sugar can be achieved by inhibiting this type of transport factor. At t...

Claims

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Application Information

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IPC IPC(8): A23L27/30A23L2/60
CPCA23L2/60A23V2002/00A23V2200/16A23V2250/21A23V2300/14
Inventor 李鹏民肖郑操
Owner NORTHWEST A & F UNIV
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