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Vaccinium bracteatum thunb extract and application thereof

A technology of Candelilla vulgaris and extract is applied in the field of Candelilla vulgaris extract to achieve the effects of high-efficiency and low-toxicity prevention, improvement of high oxidative stress state, and inhibition of retinal neovascularization.

Inactive Publication Date: 2016-09-28
HAINAN ASIA PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no research on the use of southern candle leaf and its extracts in diabetic retinopathy

Method used

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  • Vaccinium bracteatum thunb extract and application thereof
  • Vaccinium bracteatum thunb extract and application thereof
  • Vaccinium bracteatum thunb extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the preparation of southern candle leaf extract

[0030] Put 1 kg of southern candle leaves into the extraction tank, add ethanol solution with a concentration of 60%, and extract by boiling under reflux. 10 times the mass of candle leaf, filter and recover the extract. Evaporate and concentrate the extract in a rotary evaporator to obtain extract, and recover the ethanol solution. Dissolve the extract with water and stir for 20 minutes to obtain a solution. The amount of water used is 20 times the mass of the extract. Use the pretreated macroporous adsorption resin column containing 1kg, AB-8 to adsorb the solution, and the flow rate is 35ml / min; (inner diameter is 8cm) elution treatment, remove impurity, abandon this part of water eluent; Then use concentration to be 70% ethanol solution elution treatment, collect this part eluent, carry out rotary evaporation concentration to the collected eluent , and recovering ethanol, concentrating to obtain the ...

Embodiment 2

[0031] Example 2, the pharmacodynamic experiment of Nancandle leaf extract on the prevention and treatment of diabetic retinopathy

[0032] 2.1 Test material

[0033] 200 clean-grade male SD rats (purchased from Zhejiang Experimental Animal Center), weighing 150-170 g. Streptozotocin (streptozotocin, STZ, purchased from Sigma Company). Kits: GPT, GOT, ALBz, Urea, CRE, UA, GLUz (Beijing Zhongsheng Beikong Biotechnology Co., Ltd.). SOD and MDA kits were purchased from Nanjing Jiancheng Biotechnology Co., Ltd. Positive control drug Difarming Tablets (French Lekang-Medilan Pharmaceutical Factory). Main instruments: blood glucose meter (Johnson & Johnson), Hitachi 7060 automatic biochemical analyzer, Bio-Rad microplate reader, etc. Among them, the pathological sections and related tests of rat retina were completed by the Laboratory Pathology Center of Zhejiang University School of Medicine.

[0034] 2.2 Experimental modeling and grouping

[0035] 200 SD rats were randomly div...

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Abstract

The invention provides a vaccinium bracteatum thunb extract and an application thereof. Diabetic retinopathy is one of the serious complications of diabetes, which is an ocular fundus lesion with specific change as well as the most important expression of microangiopathy caused by diabetes. The vaccinium bracteatum thunb extract provided by the invention is prepared by the following steps: heating and extracting vaccinium bracteatum thunb with ethanol of certain concentration; concentrating the extract and adsorbing with a macroporous adsorption resin column; washing with water for removing impurities; eluting with a high-concentration ethanol solution; and concentrating the eluent and drying to obtain the vaccinium bracteatum thunb extract. The pharmacodynamics study indicates that the vaccinium bracteatum thunb extract provided by the invention can be used for preparing a medicine or food for preventing and treating diabetic retinopathy and realizes a remarkable prevention or treatment effect. The invention provides a new way of searching for a new medicine for treating diabetic complications.

Description

technical field [0001] The present invention relates to the use of an extract from the leaves of Candida chinensis, in particular to the extract from leaves of Candida chinensis and its use in the preparation of a composition for preventing or treating diabetic retinopathy. Background technique [0002] Diabetic retinopathy (DR) is the most important manifestation of diabetic microangiopathy. It is a fundus lesion with specific changes and one of the serious complications of diabetes. Among them, 86% of blindness in adolescents is 1 / 3 of adult blindness is caused by DR. [0003] At present, the detailed mechanism of DR has not yet been clarified. It is mainly believed that hyperglycemia induces a series of functional and biochemical metabolic abnormalities, such as blood flow changes, abnormal blood rheology, activation of polyol pathway, increased oxidative stress, and advanced glycation end-stages. Product accumulation, cytokine activation and genetic factors, etc. In ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/45A61P9/10A61P3/10A23L33/105A61K127/00
CPCA23V2002/00A61K36/45A61K2236/333A61K2236/39A61K2236/55A23V2200/328A23V2200/30A23V2250/2116A23V2300/14
Inventor 商铁存姜二晨章丽华
Owner HAINAN ASIA PHARM CO LTD
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