Immobilized Helianthus tuberosus L. fructan excision hydrolytic enzyme and preparation method thereof

Abstract

The invention discloses an immobilized Helianthus tuberosus L. fructan excision hydrolytic enzyme and a preparation method thereof. The method includes any one of the following methods that in the first method, D201 macroporous anion resin serves as a carrier of Helianthus tuberosus L. fructan excision hydrolytic enzyme liquor, after adsorption and cooling under the conditions that the temperature is 10-45 DEG C and pH is 4.0-6.0, a glutaraldehyde solution with the final concentration being 0.1-0.5% serves as a cross-linking agent, and immobilized enzyme is obtained after cross-linking, washing and drying at 2-25 DEG C; in the second method, Helianthus tuberosus L. fructan excision hydrolytic enzyme liquor is added into a sodium alginate solution to be evenly mixed, a mixed solution is prepared, the mixed solution is added into a calcium chloride solution dropwise, filtering is conducted after the mixture stands still and is immobilized, and filter cakes are washed and dried to obtain immobilized enzyme. The enzyme recycling and utilizing rate, operation stability and storage stability of the immobilized enzyme prepared through the technical scheme are remarkably increased, and accordingly the prepared immobilized enzyme has good potentials in actual industrial application.

Description

technical field [0001] The invention belongs to the field of enzyme immobilization, and in particular relates to an immobilized Jerusalem artichoke fructan exohydrolase and a preparation method thereof. Background technique [0002] Levan hydrolase refers to a class of enzymes that can catalyze the degradation of fructan. According to the different methods and sites of hydrolyzing substrates, they can be roughly divided into two categories: fructan exohydrolase and endo-inulinlase. Studies have shown that the hydrolase involved in the degradation of fructan in plants is fructan exohydrolase. Leptan exohydrolase is the main enzyme that degrades fructan in plants. The base generates 1 fructose to achieve degradation, and the final products are fructose and sucrose. Under the catalysis of invertase, the sucrose molecule can be further hydrolyzed. So far, four types of FEH have been discovered and reported: fructan 6-exohydrolase, fructan 1-exohydrolase, fructan 6&1 exohydrola...

AI Technical Summary

Problems solved by technology

[0006] The analysis of the enzymatic properties of fructan exohydrolase (Ht1-FEHII) found that pure Ht1-FEHII, like most free enzymes, has poor stability, is easily inactivated, and cannot be reused.

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